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Morphological and molecular analyses of a Meloidogyne mali population with high intragenomic rRNA polymorphism Cover

Morphological and molecular analyses of a Meloidogyne mali population with high intragenomic rRNA polymorphism

By: Jianfeng Gu,  Yiwu Fang and  Lele Liu  
Open Access
|Nov 2020

Figures & Tables

Figure 1:

Light micrographs of females and males of Meloidogyne mali. A, C, E: perineal pattern; B: whole female; D: male head; E: male tail (scale bars = 10 μm).
Light micrographs of females and males of Meloidogyne mali. A, C, E: perineal pattern; B: whole female; D: male head; E: male tail (scale bars = 10 μm).

Figure 2:

Light micrographs of juveniles of Meloidogyne mali. A: whole body of a long juvenile; B: whole body of a normal juvenile; C: anterior region; D: lateral lines; E: median bulb region; F-L: tail region. (scale bars = 10 μm).
Light micrographs of juveniles of Meloidogyne mali. A: whole body of a long juvenile; B: whole body of a normal juvenile; C: anterior region; D: lateral lines; E: median bulb region; F-L: tail region. (scale bars = 10 μm).

Figure 3:

Phylogenetic relationships within the genus Meloidogyne as inferred from Bayesian analysis of the D2-D3 region of the 28S rRNA gene sequences using the GTR + I + G model. Posterior probabilities are given in clade nodes. Newly obtained sequences are indicated in bold and the sequence codes are given in specimen-clone.
Phylogenetic relationships within the genus Meloidogyne as inferred from Bayesian analysis of the D2-D3 region of the 28S rRNA gene sequences using the GTR + I + G model. Posterior probabilities are given in clade nodes. Newly obtained sequences are indicated in bold and the sequence codes are given in specimen-clone.

Figure 5:

The TCS haplotype networks of Meloidogyne mali (A) and the Bayesian majority rule consensus tree of Meloidogyne mali based on mitochondrial COI gene (B). A: Pie chart indicates the composition and proportion of haplotypes in each location. Each circle corresponds to one haplotype and its size is proportional to its frequency. Each line connecting the haplotypes refers to a mutational step. Marks on the lines indicate the number of steps. B: Values at branche nodes are Bayesian posterior probabilities. Newly submitted sequences are indicated in bold.
The TCS haplotype networks of Meloidogyne mali (A) and the Bayesian majority rule consensus tree of Meloidogyne mali based on mitochondrial COI gene (B). A: Pie chart indicates the composition and proportion of haplotypes in each location. Each circle corresponds to one haplotype and its size is proportional to its frequency. Each line connecting the haplotypes refers to a mutational step. Marks on the lines indicate the number of steps. B: Values at branche nodes are Bayesian posterior probabilities. Newly submitted sequences are indicated in bold.

Figure 4:

Phylogenetic relationships within the genus Meloidogyne as inferred from Bayesian analysis of the ITS sequences using the GTR + I + G model. Posterior probabilities are given in clade nodes. Newly obtained sequences are indicated in bold.
Phylogenetic relationships within the genus Meloidogyne as inferred from Bayesian analysis of the ITS sequences using the GTR + I + G model. Posterior probabilities are given in clade nodes. Newly obtained sequences are indicated in bold.

Morphometrics of the described M_ mali population from Japan compares to a population detected in 2013_

M. mali population with high intragenomic rRNA polymorphism M. mali population (Gu et al., 2013)
n 2020
Body length445  ±  28.3 (401-507)425  ±  30.1 (362-466)
Body width at mid-body14.0  ±  0.6 (13.1-15.4)14.0 ± 1.1 (13.1-18.1)
Anterior end to median bulb valve52.7 ± 4.1 (46.8-58.7)53.5 ± 2.1 (49.5-56.8)
Anterior end to the hemizonid79.0 ± 3.8 (74.4-85.5)
Anterior end to the excretory pore75.1 ± 4.2 (72.8-83.3)74.1 ± 4.2 (68.8-82.3)
Head width5.8 ± 0.3 (5.3-6.4)5.1 ± 0.41 (4.1-5.7)
Head height3.0 ± 0.2 (2.6-3.4)2.6 ± 0.32 (2.1-3.2)
Stylet length10.4 ± 0.5 (9.4-11.1)10.5 ± 0.5 (9.5-11.6)
Stylet cone length5.4 ± 0.2 (5.1-5.8)5.7 ± 0.5 (4.7-6.7)
Stylet knob height1.5 ± 0.4 (1.0-2.6)1.2 ± 0.2 (0.8-1.6)
Stylet knob width2.2 ± 0.3 (1.8-2.8)2.1 ± 0.2 (1.8-2.7)
DGO3.2 ± 0.5 (2.4-4.0)4.4 ± 0.57 (3.5-5.5)
Tail length30.5 ± 4.5 (23.5-35.8)32.7 ± 3.0 (29.2-39.3)
Body width at the anus7.8 ± 0.7 (6.1-8.7)7.9 ± 0.9 (5.9-9.6)
Hyaline tail terminus length9.8 ± 1.6 (6.8-12.0)7.2 ± 2.3 (3.9-9.3)
a 31.9 ± 2.5 (27.4-36.6)30.4 ± 2.6 (25.2-34.5)
b8.0 ± 0.6 (6.8-9.2)
c 14.8 ± 2.5 (12.1-19.4)13.2 ± 1.1 (11.6-15.3)
c3.8 ± 0.6 (3.0-4.8)4.2 ± 0.7 (3.1-5.6)
m 52.4 ± 2.4 (49.5-58.6)54.5 ± 3.8 (47-60.3)
O 30.6 ± 4.7 (22.9-38.8)41.9 ± 5.8 (31.6-53.9)
Head width/head height1.9 ± 0.1 (1.7-2.2)2.0 ± 0.2 (1.6-2.3)
H%33.2 ± 8.6 (19.1-47.3)21.7 ± 5.9 (12.7-27.8)
DOI: https://doi.org/10.21307/jofnem-2020-105 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
Language: English
Page range: 1 - 11
Published on: Nov 6, 2020
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year

© 2020 Jianfeng Gu, Yiwu Fang, Lele Liu, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.