Mist chamber extraction for improved diagnosis of Meloidogyne spp. from golf course bermudagrass

Crow, William T.; Habteweld, Alemayehu; Bean, Thomas

doi:10.21307/jofnem-2020-096

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Mist chamber extraction for improved diagnosis of Meloidogyne spp. from golf course bermudagrass

Journal of Nematology

Volume 52 (2020): Issue 1 (January 2020)

By: William T. Crow, Alemayehu Habteweld and Thomas Bean

Open Access

|Nov 2020

Figures & Tables

Symptoms of Meloidogyne graminis on an ultradwarf bermudagrass golf green. Only low numbers of M. graminis vermiform stages were recovered from soil, while staining revealed the roots were infested with large numbers of sedentary stages.

Golf course bermudagrass roots infested by Meloidogyne graminis and M. marylandi typically rot off just below small galls near the soil-thatch interface, creating an extremely abbreviated root system.

The University of Florida Nematode Assay Lab mist chamber.

(A) Funnel hole cut in center shelf. (B) Water flow prevention ring. (C) Drain hole. (D) Shelf support.

(A) Misting rail. (B) Superfine mist emitter.

Samples are placed onto coffee filters on the sample support rings. Funnel necks insert slightly into the 250-ml-flaskes.

A washed turf plug including live turf, thatch and roots, ready for mist extraction.

Four washed turf plugs are placed in each funnel in the mist chamber and incubated for 72 hr.

Average number of Meloidogyne spp. vermiform stages (J2 and males) recovered from diagnostic samples submitted to the University of Florida Nematode Assay Lab using centrifugal flotation extraction from 100 cm3 of soil (Soil) and mist chamber extraction from four 3.8-cm-diam. turf plugs (Mist) from golf course bermudagrasses in Florida (N = 431), golf course bermudagrasses in Texas (N = 36), or golf course bentgrass/bluegrass in California (N = 34).

Percentage of samples submitted to the University of Florida Nematode Assay Lab from golf course bermudagrass in Florida (N = 431), golf course bermudagrass in Texas (N = 36), and golf course bentgrass/bluegrass in California (N = 34) that were classified as having no risk, low risk, moderate risk, or high risk of damage from Meloidogyne spp. and the percentage of samples that were ‘below threshold’ (no or low risk) and ‘above threshold’ (moderate or high risk) using centrifugal flotation extraction from 100 cm3 of soil (Soil) or mist chamber extraction from four 3.8-cm-diam. turf plugs (Mist). Risk thresholds used per 100 cm3 of soil or per four 3.8-cm-diam. turf plugs were 0 = no risk, 1 ≤ 79 = low risk, 80 ≤ 299 = moderate risk, ≥ 300 = high risk.

Comparison of the number of Meloidogyne spp_ J2 and males recovered from either 100 cm3 of soil using centrifugal flotation or from four 3_8-cm-diam_ turf plugs using mist extraction_

			J2				Males
Year	Cultivar	n	Soil	Mist	P <	r ²	Soil	Mist	P <	R ²
2017	Jones Dwarf	77a	6b	436	0.0001c	0.014d	1	40	0.0001	0.059
2017	Tifdwarf	49	225	945	0.0001	0.003	100	184	0.0001	0.085
2017	Tifdwarf	45	92	387	0.0001	0.002	21	92	0.0001	0.150
2017	TifEagle	59	69	368	0.0001	0.048	2	25	0.0001	0.059
2018	Tifdwarf	40	130	466	0.0001	0.005	28	129	0.0001	0.000
2019	TifEagle	71	70	723	0.0001	0.332	22	108	0.0001	0.122
2019	TifEagle	100	24	128	0.0001	0.604	2	13	0.0001	0.374
2019	Sunday	120	14	81	0.0001	0.202	<1	<1	0.0001	0.003
2020	Jones Dwarf	35	22	286	0.0001	0.560	5	26	0.0001	0.244

DOI: https://doi.org/10.21307/jofnem-2020-096 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X

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Language: English

Page range: 1 - 12

Published on: Nov 6, 2020

Published by: Society of Nematologists, Inc.

In partnership with: Paradigm Publishing Services

Publication frequency: 1 issue per year

Keywords:

Cynodon,

Related subjects:

© 2020 William T. Crow, Alemayehu Habteweld, Thomas Bean, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.

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Mist chamber extraction for improved diagnosis of Meloidogyne spp. from golf course bermudagrass

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Comparison of the number of Meloidogyne spp_ J2 and males recovered from either 100 cm3 of soil using centrifugal flotation or from four 3_8-cm-diam_ turf plugs using mist extraction_

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