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Aphelenchus yinyuensis n. sp. (Tylenchina: Aphelenchoididae) found in Terminalia sp. in China Cover

Aphelenchus yinyuensis n. sp. (Tylenchina: Aphelenchoididae) found in Terminalia sp. in China

Open Access
|Sep 2020

Figures & Tables

Figure 1:

Aphelenchus yinyuensis n. sp. A: Entire male; B: Entire female; C: Anterior region; D: Lip region; E: Vulval region lateral view; F: Vulval region ventral view; G: Female reproductive system; H, I: Female tails; J: Male tail. Scale bars (A, B = 20 μm; C-J = 10 μm).
Aphelenchus yinyuensis n. sp. A: Entire male; B: Entire female; C: Anterior region; D: Lip region; E: Vulval region lateral view; F: Vulval region ventral view; G: Female reproductive system; H, I: Female tails; J: Male tail. Scale bars (A, B = 20 μm; C-J = 10 μm).

Figure 2:

Light photomicrographs of Aphelenchus yinyuensis n. sp. A: Entire male; B: Entire female; C: Anterior region: D: Lip region; E: Lateral lines; F: Vulval region lateral view; G: Vulval region ventral view; H: Vulval region with post-uterine sac; I: Female reproductive system; J, K: Female tails; L, M: Male tail showing papillae arrangement (arrowheads). (ex = excretory pore; h = hemizonid; P + number = genital papillae; scale bars = A, B = 20 μm; C-M = 10 μm).
Light photomicrographs of Aphelenchus yinyuensis n. sp. A: Entire male; B: Entire female; C: Anterior region: D: Lip region; E: Lateral lines; F: Vulval region lateral view; G: Vulval region ventral view; H: Vulval region with post-uterine sac; I: Female reproductive system; J, K: Female tails; L, M: Male tail showing papillae arrangement (arrowheads). (ex = excretory pore; h = hemizonid; P + number = genital papillae; scale bars = A, B = 20 μm; C-M = 10 μm).

Figure 3:

Phylogenetic relationship of Aphelenchus yinyuensis n. sp. and other nematodes based on near full length 18S of rDNA. Bayesian tree inferred from 18S under GTR + I + G model (lnL = −19,759.9398; freqA = 0.2462; freqC = 0.2010; freqG = 0.2710; freqT = 0.2818; R(a) = 1.1079; R(b) = 2.1894; R(c) = 1.2504; R(d) = 0.7956; R(e) = 3.9256; R(f) = 1.0000; Pinvar = 0.1260; Shape = 0.5890). Posterior probability values exceeding 50% are given on appropriate clades.
Phylogenetic relationship of Aphelenchus yinyuensis n. sp. and other nematodes based on near full length 18S of rDNA. Bayesian tree inferred from 18S under GTR + I + G model (lnL = −19,759.9398; freqA = 0.2462; freqC = 0.2010; freqG = 0.2710; freqT = 0.2818; R(a) = 1.1079; R(b) = 2.1894; R(c) = 1.2504; R(d) = 0.7956; R(e) = 3.9256; R(f) = 1.0000; Pinvar = 0.1260; Shape = 0.5890). Posterior probability values exceeding 50% are given on appropriate clades.

Figure 4:

Phylogenetic relationship of Aphelenchus yinyuensis n. sp. and other nematodes based on 28S D2 to D3 of rDNA. Bayesian tree inferred from 28S D2 to D3 under GTR + I + G model (lnL = −17,543.4883; freqA = 0.1670; freqC = 0.1883; freqG = 0.3314; freqT = 0.3134; R(a) = 1.3541; R(b) = 3.5721; R(c) = 1.7006; R(d) = 0.5742; R(e) = 4.6218; R(f) = 1.0000; Pinvar = 0.1400; Shape = 0.6940). Posterior probability values exceeding 50% are given on appropriate clades.
Phylogenetic relationship of Aphelenchus yinyuensis n. sp. and other nematodes based on 28S D2 to D3 of rDNA. Bayesian tree inferred from 28S D2 to D3 under GTR + I + G model (lnL = −17,543.4883; freqA = 0.1670; freqC = 0.1883; freqG = 0.3314; freqT = 0.3134; R(a) = 1.3541; R(b) = 3.5721; R(c) = 1.7006; R(d) = 0.5742; R(e) = 4.6218; R(f) = 1.0000; Pinvar = 0.1400; Shape = 0.6940). Posterior probability values exceeding 50% are given on appropriate clades.

Morphometrics of Aphelenchus yinyuensis n_ sp_

FemaleMale
CharacterHolotypeParatypesParatypes
n1515
L792793 ± 71.9 (639-877)756 ± 59.6 (647-863)
a31.030.5 ± 2.7 (26.9-33.6)31.9 ± 3.4 (26.7-37.0)
b9.67.7 ± 1.0 (6.2-8.8)7.5 ± 0.7 (6.1-8.7)
b′5.04.8 ± 0.6 (3.9-5.7)4.5 ± 0.4 (3.8-5.3)
c19.521.0 ± 3.1 (16.7-27.1)26.7 ± 2.6 (22.1-30.4)
c′3.12.7 ± 0.4 (2.3-3.8)1.8 ± 0.2 (1.5-2.2)
V or T72.772.8 ± 3.8 (65.2-77.6)70.5 ± 7.1 (59.4-89.0)
Lip region height3.13.6 ± 0.3 (3.1-4.2)3.4 ± 0.5 (2.5-4.2)
Lip region width8.38.8 ± 0.5 (8.1-9.5)8.5 ± 0.5 (7.8-9.5)
Stylet length13.714.3 ± 0.7 (13.2-15.2)13.8 ± 1.0 (12.2-15.7)
Body diam.25.526.2 ± 3.1 (19.2-31.7)23.9 ± 2.1 (19.2-28.2)
Median bulb width15.316.1 ± 1.5 (13.1-19.6)13.8 ± 1.0 (12.2-15.7)
Median bulb length22.522.3 ± 1.8 (18.6-24.5)20.1 ± 1.2 (17.8-21.9)
Median bulb length/diam. Ratio1.51.4 ± 0.1 (1.2-1.5)1.5 ± 0.1 (1.3-1.7)
E. pore from anterior end113.6107.9 ± 7.2 (96.6-123.3)106.5 ± 7.2 (91.5-116.7)
Ovary length or Testis436.3407.4 ± 50.2 (280.2-458.3)531.4 ± 54.1 (452.6-610.9)
Post-uterine sac112.1100.7 ± 15.5 (66.3-120.2)
Vulva to anus distance176.3166.9 ± 22.3 (131.2-195.0)
Post-uterine sac length/vulva to anus (%)63.660.9 ± 10.4 (44.5-82.7)
Anal (cloacal) body diameter13.114.2 ± 2.0 (10.6-17.3)15.4 ± 1.0 (13.9-17.3)
Tail length40.638.4 ± 5.9 (24.2-44.9)28.4 ± 2.4 (23.6-32.2)
Spicule (curved median line)28.0 ± 2.0 (24.3-31.3)
Spicule (Chord)28.7 ± 1.7 (25.8-32.3)
Gubernaculum13.2 ± 0.4 (12.2-13.7)
Hemizonid115.3110.1 ± 5.5 (100.1-117.3)108.5 ± 7.3 (96.1-121.3)
Head to pharyngo-intestinal junction92.9103.2 ± 6.9 (91.0-113.5)100.6 ± 5.5 (86.0-109.3)
Head to pharyngeal gland end159.6163.9 ± 12.1 (139.6-181.0)165.9 ± 12.0 (149.3-188.2)
Vulva to tail terminus203.1213.1 ± 28.6 (156.3-251.8)
DOI: https://doi.org/10.21307/jofnem-2020-085 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
Language: English
Page range: 1 - 12
Published on: Sep 3, 2020
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year

© 2020 Gu Jianfeng, Munawar Maria, Yiwu Fang, Liu Lele, Xianfeng Chen, Bo Cai, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.