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Description of Deladenus gilanica n. sp. (Hexatylina: Neotylenchidae) isolated from wood of black pine in Northern Iran

Journal of Nematology
Volume 52 (2020): Issue 1 (January 2020)
By:
Open Access
|Jul 2020

Figures & Tables

Figure 1:

Line drawings of Deladenus gilanica n. sp. (A-F) Mycophagous female − (A) entire body; (B) pharyngeal region; (C) vulva region; (D) anterior end; (E, F) posterior body (tail). (G and H) Infective female − (G) entire body; (H) anterior end (Scale bars: A, G = 20 µm, B-F, H = 10 µm).
Line drawings of Deladenus gilanica n. sp. (A-F) Mycophagous female − (A) entire body; (B) pharyngeal region; (C) vulva region; (D) anterior end; (E, F) posterior body (tail). (G and H) Infective female − (G) entire body; (H) anterior end (Scale bars: A, G = 20 µm, B-F, H = 10 µm).

Figure 2:

Photomicrographs of Deladenus gilanica n. sp. (A-D, F and G) Mycophagous female − (A) pharyngeal region; (B) anterior end; (C) vulva region; (D) pharyngeal region showing excretory pore and hemizonid; (F) end of female pharyngeal region; (G) female posterior body (tail). (E, H, and I) Infective female − (E) anterior end; (H) vulva region; (I) posterior body (tail) (All scale bars = 10 µm).
Photomicrographs of Deladenus gilanica n. sp. (A-D, F and G) Mycophagous female − (A) pharyngeal region; (B) anterior end; (C) vulva region; (D) pharyngeal region showing excretory pore and hemizonid; (F) end of female pharyngeal region; (G) female posterior body (tail). (E, H, and I) Infective female − (E) anterior end; (H) vulva region; (I) posterior body (tail) (All scale bars = 10 µm).

Figure 3:

The 10001st Bayesian tree inferred from 18S under GTR + I + G model (−lnL = 3768.1687; AIC = 7556.3374; freqA = 0.2621; freqC = 0.1952; freqG = 0.2673; freqT = 0.2755; R(a) = 1.4504; R(b) = 3.0092; R(c) = 1.5594; R(d) = 0.3743; R(e) = 7.8701; R(f) = 1; Pinva = 0.4966; Shape = 0.6306). Posterior probability values exceeding 50% are given on appropriate clades.
The 10001st Bayesian tree inferred from 18S under GTR + I + G model (−lnL = 3768.1687; AIC = 7556.3374; freqA = 0.2621; freqC = 0.1952; freqG = 0.2673; freqT = 0.2755; R(a) = 1.4504; R(b) = 3.0092; R(c) = 1.5594; R(d) = 0.3743; R(e) = 7.8701; R(f) = 1; Pinva = 0.4966; Shape = 0.6306). Posterior probability values exceeding 50% are given on appropriate clades.

Figure 4:

The 10001st Bayesian tree inferred from Revised 28S D2/D3 under TVM + I + G model (−lnL = 3258.8145; AIC = 6535.6289; freqA = 0.1843; freqC = 0.2082; freqG = 0.3284; freqT = 0.2791; R(a) = 1.3629; R(b) = 4.8955; R(c) = 2.5959; R(d) = 0.7212; R(e) = 4.8955; R(f) = 1; Pinva = 0.2327; Shape = 0.4722). Posterior probability values exceeding 50% are given on appropriate clades.
The 10001st Bayesian tree inferred from Revised 28S D2/D3 under TVM + I + G model (−lnL = 3258.8145; AIC = 6535.6289; freqA = 0.1843; freqC = 0.2082; freqG = 0.3284; freqT = 0.2791; R(a) = 1.3629; R(b) = 4.8955; R(c) = 2.5959; R(d) = 0.7212; R(e) = 4.8955; R(f) = 1; Pinva = 0.2327; Shape = 0.4722). Posterior probability values exceeding 50% are given on appropriate clades.

Morphometrics of female of Deladenus gilanica n_ sp_ from Iran_

Mycetophagous stageInfective stage
CharactersHolotype femaleParatype femalesParatype females
n −104
L367364.8 ± 33.7 (314-422)330.3 ± 17.6 (310-341)
L'361335.4 ± 33.3 (285-393)294.7 ± 15.3 (277-304)
a18.621.1 ± 2.6 (18.1-26.5)28.3 ± 0.1 (28.2-28.4)
b8.57.1 ± 0.9 (5.9-8.5)6.2 ± 1.8 (5.1-8.3)
b'5.75.0 ± 0.9 (4.1-6.9)3.7 ± 0.4 (3.4-4.1)
c13.412.4 ± 1.1 (10.8-14.6)9.3 ± 0.3 (9.0-9.4)
c'2.43.3 ± 0.4 (2.4-3.8)5.1 ± 0.4 (4.7-5.4)
V87.486.6 ± 0.7 (85.4-87.4)82.6 ± 0.6 (82.1-83.2)
Head height11.4 ± 0.2 (1-1.5)2.1 ± 0.3 (2-2.5)
Head width66.5 ± 0.5 (6-7)4.8 ± 0.3 (4.5-5)
Stylet88.0 ± 0.2 (7.5-8.0)13.9 ± 0.9 (13-15)
Excretory pore7168.4 ± 5.2 (61-76)69 ± 6.5 (63-76)
Hemizonid7168.4 ± 5.2 (61-76)68.5 ± 6.6 (63-76)
Pharynx4651.4 ± 4.9 (46-63)54 ± 10.6 (41-64)
Overlapping2223.1 ± 7.5 (11-33)32.3 ± 5.9 (29-41)
Body width2117.5 ± 2.8 (14-22)11.8 ± 0.5 (11-12)
Head-vulva341316.0 ± 30.8 (268-367)272.7 ± 12.7 (258-281)
Vulva-anus2019.4 ± 3.4 (15-26)22 ± 3.0 (19-25)
Tail2929.4 ± 1.3 (27-32)35.7 ± 2.5 (33-38)

Comparison of Deladenus gilanica n_ sp_ with eight similar species_

SpeciesLabcVStyletMedian corporeal chamberExcretory pore relative to hemizonida TailLib MaleInfective stage
D. gilanica n. sp.314-42218.1-26.55.9-8.510.8-14.685.4-87.47.5-8–SL27-328 at level of vulva–+
D. durus 620-13,6021-507.7-1619.5-39.390-956-11+P21-436-7+–
D. aridus 69331.2 19.891.99.4–P 5––
D. obesus 970-1,40018-268-16.932-33.692-937.3-9–P30-478-10––
D. processus 760-99034-4913.3-17.719.6-22.892.2-93.56-7–P35-446+–
D. oryzae 660-96019-267.92992-93.57-9+A29-34Indistinct++
D. wilsoni 1,490-2,70034.6-62.615.5-26.546.6-66.994.6-96.210-11–A or SL 7-9 at level of vulva++
D. proximus 1,760-2,20040-53.716.3-21.344-6095.1-95.911-12–A31-477-8 at level of vulva++
D. posteroporus 889-1,02638-4310.1-12.831.3-37.193.5-95.98-11–A27-2911-12 at midbody++
DOI: https://doi.org/10.21307/jofnem-2020-065 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
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Language: English
Page range: 1 - 10
Submitted on: Jan 12, 2020
Published on: Jul 28, 2020
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year
Keywords:
Molecular,
Morphology,
Morphometrics,
Nematode,
New species,
rRNA gene
Related subjects:
Life sciences,
Life sciences, other

© 2020 Parisa Jalalinasab, Mehrab Esmaeili, Weimin Ye, Ramin Heydari, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.

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