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Description of Deladenus brevis n. sp. (Sphaerularioidea: Neotylenchidae) from Iran: a morphological and molecular phylogenetic study

Journal of Nematology
Volume 52 (2020): Issue 1 (January 2020)
By:
Open Access
|Jul 2020

Figures & Tables

Figure 1:

Line drawings of Deladenus brevis n. sp. (A, B) Pharynx of male and female; (C) Anterior end of female; (D) Female genital tract and posterior body region; (E) Male posterior body region; (F) Lateral lines; (G) Female tail.
Line drawings of Deladenus brevis n. sp. (A, B) Pharynx of male and female; (C) Anterior end of female; (D) Female genital tract and posterior body region; (E) Male posterior body region; (F) Lateral lines; (G) Female tail.

Figure 2:

Photomicrographs of Deladenus brevis n. sp. (A, B) Anterior body region of female (fresh sample in water and permanently preserved specimen in glycerin respectively); (C) Part of pharynx (arrowhead showing excretory pore and C1 showing details of stylet); (D) Part of female reproductive system; (E) Female posterior body region; (F1 and F2) Lateral vulval flaps in ventrolateral and lateral view in two specimens; (G) Lateral lines; (H) Tip of ovary (arrowhead); (I, J) Male posterior body (Scale bars: A-F2, H-J =10 µm, F1, C1, G =5 µm).
Photomicrographs of Deladenus brevis n. sp. (A, B) Anterior body region of female (fresh sample in water and permanently preserved specimen in glycerin respectively); (C) Part of pharynx (arrowhead showing excretory pore and C1 showing details of stylet); (D) Part of female reproductive system; (E) Female posterior body region; (F1 and F2) Lateral vulval flaps in ventrolateral and lateral view in two specimens; (G) Lateral lines; (H) Tip of ovary (arrowhead); (I, J) Male posterior body (Scale bars: A-F2, H-J =10 µm, F1, C1, G =5 µm).

Figure 4:

Bayesian 50% majority rule consensus tree of Deladenus brevis n. sp. based on SSU rDNA sequences under GTR + I + G model. Bayesian posterior probability values more than 0.50 are given for appropriate clades. The new sequence is indicated in bold.
Bayesian 50% majority rule consensus tree of Deladenus brevis n. sp. based on SSU rDNA sequences under GTR + I + G model. Bayesian posterior probability values more than 0.50 are given for appropriate clades. The new sequence is indicated in bold.

Figure 3:

Deladenus brevis n. sp. (Scanning Electron Microscopy [SEM]). (A-C) Anterior end in ventral, sublateral and frontal views, respectively. (D) Deirid (white arrow) and excretory pore in lateral view (black arrow). (E) Excretory pore in ventral view (arrow). (F) Lateral field. (G, H) Vulva in lateral and ventral views, respectively. (I) Anus in ventral view (arrow). (J, K) Female posterior end in lateral and ventral views, respectively (arrow pointing the anus). (L, M) Male posterior end in lateral and subventral views, respectively.
Deladenus brevis n. sp. (Scanning Electron Microscopy [SEM]). (A-C) Anterior end in ventral, sublateral and frontal views, respectively. (D) Deirid (white arrow) and excretory pore in lateral view (black arrow). (E) Excretory pore in ventral view (arrow). (F) Lateral field. (G, H) Vulva in lateral and ventral views, respectively. (I) Anus in ventral view (arrow). (J, K) Female posterior end in lateral and ventral views, respectively (arrow pointing the anus). (L, M) Male posterior end in lateral and subventral views, respectively.

Figure 5:

Bayesian 50% majority rule consensus tree of Deladenus brevis n. sp. based on LSU rDNA D2 to D3 sequences under GTR + I + G model. Bayesian posterior probability values more than 0.50 are given for appropriate clades. The new sequence is indicated in bold.
Bayesian 50% majority rule consensus tree of Deladenus brevis n. sp. based on LSU rDNA D2 to D3 sequences under GTR + I + G model. Bayesian posterior probability values more than 0.50 are given for appropriate clades. The new sequence is indicated in bold.

Figure 6.

Bayesian 50% majority rule consensus tree of Deladenus brevis n. sp. based on COI mtDNA sequences under GTR + I + G model. Bayesian posterior probability values more than 0.50 are given for appropriate clades. The new sequence is indicated in bold.
Bayesian 50% majority rule consensus tree of Deladenus brevis n. sp. based on COI mtDNA sequences under GTR + I + G model. Bayesian posterior probability values more than 0.50 are given for appropriate clades. The new sequence is indicated in bold.

Morphometrics of Deladenus brevis n_ sp_

FemaleMale
Character

  • Holotype

  • Paratypes

  • Paratypes

n −8

  • 2

L 435

  • 415.5 ± 27.0 (367-454)

  • 391, 350

  • a

26.028 ± 3 (22.3-32.3)

  • 35.5, 29.1

  • b

9.58.9 ± 0.7 (8.0-10.3)

  • 8.0, 7.6

  • c

15.0

  • 16.8 ± 2.0 (14.3-20.0)

  • 17.7, 15.2

  • c′

3.22.8 ± 0.3 (2.4-3.2)

  • 2.7, 2.9

T or V 88.989.0 ± 1.0 (87.2-90.3)

  • 82.3, 85.3

  • m

39.5

  • 39.0 ± 3.0 (33.3-43.1)

  • 50.9, 46.3

  • Cephalic region height

1.41.9 ± 0.2 (1.5-2.1)

  • 2.0, 1.5

  • Cephalic region width

5.75.9 ± 0.4 (5.0-6.5)

  • 5.2, 6

  • Max. body dim.

1715 ± 2 (13-18)

  • 11, 13

  • Stylet total length

6.66.4 ± 0.3 (6-7)

  • 6.0, 6.5

  • Stylet conus

2.62.5 ± 0.2 (2-3)

  • 2.2, 2.5

  • Excretory pore from anterior

7879.0 ± 5.3 (67-86)

  • 75.0, 70.5

  • Nerve ring from anterior

71

  • 71.5 ± 9.0 (60-83)

  • 52, ?

  • Hemizonid from anterior

7677.5 ± 6.0 (66-86)

  • 72, 69

  • Vulval body diameter

1312.5 ± 2. (10-18)

  • −

  • Anal body diameter

99.0 ± 1.2 (7-10)

  • 8, 9

  • Tail

2925.0 ± 3.4 (20-30)

  • 22, 23

  • Spicules

−−

  • 11.3, 14.5

  • Gubernaculum

−−

  • 3.4, 4.5

DOI: https://doi.org/10.21307/jofnem-2020-062 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
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Language: English
Page range: 1 - 13
Submitted on: Mar 26, 2020
Published on: Jul 28, 2020
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year
Keywords:
mtDNA,
Free living mycetophagous phase,
LSU rDNA D2-D3,
Phylogeny,
SSU rDNA,
Taxonomy
Related subjects:
Life sciences,
Life sciences, other

© 2020 Fariba Heydari, Joaquín Abolafia, Majid Pedram, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.

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