Figure 1:
Iranian population of Ektaphelenchoides pini (Massey, 1966; Baujard, 1984). A: Anterior body region of male; B: Anterior body region of female; C: Metacorpus and the position of E pore (arrow) (male); D: Vulval region and PUS; E: Vulval slit in ventral view; F: Ventro-lateral view of male caudal region showing P2 (upper arrows) and P3 (lower arrows) papillae; G: Tip of ovary; H: Female posterior body region; I: Male cloacal region and spicule and tail tip (I1); J: Lateral lines; K: Spicule (close up), L: Male posterior body region showing P2 (upper arrow) and P3 (lower arrow) and single ventral P1 lacking. (Scale bars = 10 µm).
Table 1.
Morphometrics of Ektaphelenchoides pini (Massey, 1966; Baujard, 1984) from Golestan province, Iran, and comparison with data of the type population and the population from France.
| This study | Type population, studied by Baujard (1984) | French population (Baujard, 1984) | ||||
|---|---|---|---|---|---|---|
| Character | Females | Males | Female | Males | Females | Males |
| n | 14 | 9 | 1 | 10 | 40 | 5 |
| L | 829.4 ± 70.2 | 611.8 ± 59.6 | 720 | 840 | 740 | 750 |
| (756–947) | (502–701) | (730–980) | (630–950) | (680–820) | ||
| a | 37.4 ± 3.1 | 38.8 ± 3.2 | 33 | 32.5 | 33 | 33 |
| (30.0–37.7) | (33.8–39.6) | (28–38) | (31–36) | (31–36) | ||
| b | 7.2 ± 0.8 | 6.7 ± 0.7 | 9 | 8 | 8 | 8 |
| (6.03–8.2) | 4.3 ± 0.8 | (7–9) | (7–11) | (7–9) | ||
| b´ | 4.7 ± 0.5 | (3.3–5.8) | – | – | – | – |
| (4–5.6) | 13.9 ± 1.1 | |||||
| c | – | (12.5–15.8) | – | 14 | – | 14 |
| 2.9 ± 0.2 | (13–17) | (12.5–118.0) | ||||
| c´ | – | 2.9 ± 0.2 | – | 2.9 | – | 3.1 |
| (2.7–3.1) | (2.6–3.0) | (2.2–3.6) | ||||
| T or V | 69.4 ± 2.0 | 90.6 ± 1.9 | 74 | – | 70 | – |
| (70.2–72.3) | (87.0–88.6) | (67–74) | ||||
| Cephalic region height | 3.6 ± 0.4 | 3.0 ± 0.4 | – | – | – | – |
| (3.0-4.3) | (2.4-3.7) | |||||
| Cephalic region width | 8.2 ± 0.5 | 7.4 ± 0.6 | – | – | – | – |
| (7.4–8.8) | (6.4–8.5) | |||||
| Stylet | 20.9 ± 1.1 | 18.7 ± 1.0 | 23 | 24 | 23 | 23 |
| (20–23) | (17.3–19.8) | (21–26) | (20–26) | (22–24) | ||
| Max. body dim. | 22.4 ± 2.2 | 15.8 ± 1.2 | – | – | – | – |
| (20–26) | (14–18) | |||||
| m | 40.4 ± 5.0 | 44.4 ± 4.9 | – | – | – | – |
| (32.5–44.0) | (36.6–47.4) | |||||
| Body width at MB | 17.1 ± 1.4 | 15.0 ± 1.2 | – | – | – | – |
| (16–20) | (13–17) | |||||
| E pore from anterior | 97.5 ± 5.4 | 99.2 ± 8.3 | 104 | 119 | 105 | |
| end | (92–106) | (91.2–108.1) | (101–132) | (94–123) | ||
| Nerve ring from | 100.5 ± 7.5 | 85.8 ± 14.2 | – | – | – | – |
| anterior end | (87–97) | (71–110) | ||||
| Median bulb width | 11.1 ± 1.4 | 9.9 ± 1.5 | – | – | – | – |
| (10–14) | (8–12) | |||||
| Median bulb length | 20.7 ± 2.7 | 18.3 ± 2.3 | – | – | – | – |
| (17–25) | (16–22) | |||||
| Median bulb length/ | 1.9 ± 0.2 | 1.8± 0.1 | – | – | – | – |
| diam. ratio | (1.5–2.2) | (1.7–2.0) | ||||
| Ovary/testis length | 360.5 ± 34.7 | 197.8± 6.6 | – | – | – | – |
| (310–420) | (184–201) | |||||
| Hemizonid from | 98 ± 3 | 104.4 ± 7.9 | – | – | 109 | – |
| anterior end | (92–105) | (93–109) | (97–115) | |||
| Vulval body diam. | 22.3 ± 3.1 | – | – | – | – | – |
| (17–28) | ||||||
| PUS length | 27.6 ± 3.3 | – | – | – | ? | – |
| (23–33) | ||||||
| Anal/cloacal body | – | 15.1 ± 1.5 | – | – | – | – |
| diam. | (13–18) | |||||
| Tail | – | 44.0 ± 3.1 | – | – | – | 53 |
| (40–49) | (38–61) | |||||
| Spicules arc line | – | 20.6 ± 1.0 | 25.5 | 24 | ||
| length | (19–22) | (22–28) | (21–26) | |||
| Capitulum width | – | 5.6 ± 0.5 | – | – | – | – |
| (5-6) | ||||||
Figure 2:
Bayesian 50% majority rule consensus tree inferred from analysis of the partial SSU rDNA sequence of Iranian population of Ektaphelenchoides pini (Massey, 1966; Baujard, 1984) from Golestan province under the GTR + G + I model. Bayesian posterior probability values more than 0.50 are given for appropriate clades. The new sequence is indicated in bold.
Figure 3:
Bayesian 50% majority rule consensus tree inferred from analysis of the partial LSU rDNA sequence of Iranian population of Ektaphelenchoides pini (Massey, 1966; Baujard, 1984) from Golestan province under the GTR + G + I model. Bayesian posterior probability values more than 0.50 are given for appropriate clades. The new sequence is indicated in bold.