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Morphological and molecular characterization of Ektaphelenchoides pini (Massey, 1966) Baujard, 1984 (Aphelenchoididae; Ektaphelenchinae) from Iran, with morphological and taxonomic observations on some species

Open Access
|May 2020

Figures & Tables

Figure 1:

Iranian population of Ektaphelenchoides pini (Massey, 1966; Baujard, 1984). A: Anterior body region of male; B: Anterior body region of female; C: Metacorpus and the position of E pore (arrow) (male); D: Vulval region and PUS; E: Vulval slit in ventral view; F: Ventro-lateral view of male caudal region showing P2 (upper arrows) and P3 (lower arrows) papillae; G: Tip of ovary; H: Female posterior body region; I: Male cloacal region and spicule and tail tip (I1); J: Lateral lines; K: Spicule (close up), L: Male posterior body region showing P2 (upper arrow) and P3 (lower arrow) and single ventral P1 lacking. (Scale bars = 10 µm).
Iranian population of Ektaphelenchoides pini (Massey, 1966; Baujard, 1984). A: Anterior body region of male; B: Anterior body region of female; C: Metacorpus and the position of E pore (arrow) (male); D: Vulval region and PUS; E: Vulval slit in ventral view; F: Ventro-lateral view of male caudal region showing P2 (upper arrows) and P3 (lower arrows) papillae; G: Tip of ovary; H: Female posterior body region; I: Male cloacal region and spicule and tail tip (I1); J: Lateral lines; K: Spicule (close up), L: Male posterior body region showing P2 (upper arrow) and P3 (lower arrow) and single ventral P1 lacking. (Scale bars = 10 µm).

Figure 2:

Bayesian 50% majority rule consensus tree inferred from analysis of the partial SSU rDNA sequence of Iranian population of Ektaphelenchoides pini (Massey, 1966; Baujard, 1984) from Golestan province under the GTR + G + I model. Bayesian posterior probability values more than 0.50 are given for appropriate clades. The new sequence is indicated in bold.
Bayesian 50% majority rule consensus tree inferred from analysis of the partial SSU rDNA sequence of Iranian population of Ektaphelenchoides pini (Massey, 1966; Baujard, 1984) from Golestan province under the GTR + G + I model. Bayesian posterior probability values more than 0.50 are given for appropriate clades. The new sequence is indicated in bold.

Figure 3:

Bayesian 50% majority rule consensus tree inferred from analysis of the partial LSU rDNA sequence of Iranian population of Ektaphelenchoides pini (Massey, 1966; Baujard, 1984) from Golestan province under the GTR + G + I model. Bayesian posterior probability values more than 0.50 are given for appropriate clades. The new sequence is indicated in bold.
Bayesian 50% majority rule consensus tree inferred from analysis of the partial LSU rDNA sequence of Iranian population of Ektaphelenchoides pini (Massey, 1966; Baujard, 1984) from Golestan province under the GTR + G + I model. Bayesian posterior probability values more than 0.50 are given for appropriate clades. The new sequence is indicated in bold.

Morphometrics of Ektaphelenchoides pini (Massey, 1966; Baujard, 1984) from Golestan province, Iran, and comparison with data of the type population and the population from France_

This studyType population, studied by Baujard (1984) French population (Baujard, 1984)
CharacterFemalesMalesFemaleMalesFemalesMales
n149110405
L829.4 ± 70.2611.8 ± 59.6720840740750
(756–947)(502–701) (730–980)(630–950)(680–820)
a37.4 ± 3.138.8 ± 3.23332.53333
(30.0–37.7)(33.8–39.6) (28–38)(31–36)(31–36)
b7.2 ± 0.86.7 ± 0.79888
(6.03–8.2)4.3 ± 0.8 (7–9)(7–11)(7–9)
4.7 ± 0.5(3.3–5.8)
(4–5.6)13.9 ± 1.1
c(12.5–15.8)1414
2.9 ± 0.2 (13–17) (12.5–118.0)
2.9 ± 0.22.93.1
(2.7–3.1) (2.6–3.0) (2.2–3.6)
T or V69.4 ± 2.090.6 ± 1.97470
(70.2–72.3)(87.0–88.6) (67–74)
Cephalic region height3.6 ± 0.43.0 ± 0.4
(3.0-4.3)(2.4-3.7)
Cephalic region width8.2 ± 0.57.4 ± 0.6
(7.4–8.8)(6.4–8.5)
Stylet20.9 ± 1.118.7 ± 1.023242323
(20–23)(17.3–19.8) (21–26)(20–26)(22–24)
Max. body dim.22.4 ± 2.215.8 ± 1.2
(20–26)(14–18)
m40.4 ± 5.044.4 ± 4.9
(32.5–44.0)(36.6–47.4)
Body width at MB17.1 ± 1.415.0 ± 1.2
(16–20)(13–17)
E pore from anterior97.5 ± 5.499.2 ± 8.3104119105
end(92–106)(91.2–108.1) (101–132)(94–123)
Nerve ring from100.5 ± 7.585.8 ± 14.2
anterior end(87–97)(71–110)
Median bulb width11.1 ± 1.49.9 ± 1.5
(10–14)(8–12)
Median bulb length20.7 ± 2.718.3 ± 2.3
(17–25)(16–22)
Median bulb length/1.9 ± 0.21.8± 0.1
diam. ratio(1.5–2.2)(1.7–2.0)
Ovary/testis length360.5 ± 34.7197.8± 6.6
(310–420)(184–201)
Hemizonid from98 ± 3104.4 ± 7.9109
anterior end(92–105)(93–109) (97–115)
Vulval body diam.22.3 ± 3.1
(17–28)
PUS length27.6 ± 3.3?
(23–33)
Anal/cloacal body15.1 ± 1.5
diam. (13–18)
Tail44.0 ± 3.153
(40–49) (38–61)
Spicules arc line20.6 ± 1.0 25.5 24
length (19–22) (22–28) (21–26)
Capitulum width5.6 ± 0.5
(5-6)
DOI: https://doi.org/10.21307/jofnem-2020-052 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
Language: English
Page range: 1 - 12
Submitted on: Mar 2, 2020
Published on: May 25, 2020
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year

© 2020 Fariba Heydari, Majid Pedram, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.