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Cephalenchus driekieae n. sp. (Nematoda: Tylenchidae) from South Africa, a new member of the genus with a long pharyngeal overlap

Open Access
|Apr 2020

Figures & Tables

Figure 1:

Line drawings of Cephalenchus driekieae n. sp. from South Africa, female. (A) Entire body; (B) anterior region; (C) pharyngeal region; (D) reproductive system; (E) tail.
Line drawings of Cephalenchus driekieae n. sp. from South Africa, female. (A) Entire body; (B) anterior region; (C) pharyngeal region; (D) reproductive system; (E) tail.

Figure 2:

Light micrographs of Cephalenchus driekieae n. sp. from South Africa, female. (A) Entire body; (B) pharyngeal region; (C) lateral lines; (D) part of reproduction system; (E) anterior region; (F) pharyngeal overlap; (G) tail; (H) tail terminus. (Scale bars = 10 µm).
Light micrographs of Cephalenchus driekieae n. sp. from South Africa, female. (A) Entire body; (B) pharyngeal region; (C) lateral lines; (D) part of reproduction system; (E) anterior region; (F) pharyngeal overlap; (G) tail; (H) tail terminus. (Scale bars = 10 µm).

Figure 3:

Scanning electron microscopy of Cephalenchus driekieae n. sp. from South Africa, female. (A) Anterior region; (B) lip region; (C) posterior body region; (D, E) vulval region in lateral and ventral view; (F) anus and lateral lines in anus region.
Scanning electron microscopy of Cephalenchus driekieae n. sp. from South Africa, female. (A) Anterior region; (B) lip region; (C) posterior body region; (D, E) vulval region in lateral and ventral view; (F) anus and lateral lines in anus region.

Figure 4:

Bayesian 50% majority rule consensus tree inferred from small subunit (SSU) rDNA gene sequence of Cephalenchus driekieae n. sp. from South Africa under GTR + I + G model (lnL = 6,436.7202; freqA = 0.2443; freqC = 0.2268; freqG = 0.2864; freqT = 0.2425; rAC = 1.0699; rAG = 2.8891; rAT = 1.2180; rCG = 1.1273; rCT = 5.8691; Pinv = 0.4073; Alpha = 0.6044). Bayesian posterior probability (BPP) values >0.50 are given for appropriate clades. The sequence of the new species is indicated by bold font.
Bayesian 50% majority rule consensus tree inferred from small subunit (SSU) rDNA gene sequence of Cephalenchus driekieae n. sp. from South Africa under GTR + I + G model (lnL = 6,436.7202; freqA = 0.2443; freqC = 0.2268; freqG = 0.2864; freqT = 0.2425; rAC = 1.0699; rAG = 2.8891; rAT = 1.2180; rCG = 1.1273; rCT = 5.8691; Pinv = 0.4073; Alpha = 0.6044). Bayesian posterior probability (BPP) values >0.50 are given for appropriate clades. The sequence of the new species is indicated by bold font.

Figure 5:

Bayesian 50% majority rule consensus tree inferred from D2-D3 expansion segments of large subunit (LSU) rDNA gene sequence of Cephalenchus driekieae n. sp. from South Africa under GTR + I + G model (lnL = 7,632.2964; freqA = 0.1794; freqC = 0.2463; freqG = 0.3573; freqT = 0.2170; rAC = 0.9583; rAG = 2.6626; rAT = 1.2424; rCG = 0.6711; rCT = 5.4981; Pinv = 0.2528; Alpha = 0.6707). Bayesian posterior probability (BPP) values >0.50 are given for appropriate clades. The sequence of the new species is indicated by bold font.
Bayesian 50% majority rule consensus tree inferred from D2-D3 expansion segments of large subunit (LSU) rDNA gene sequence of Cephalenchus driekieae n. sp. from South Africa under GTR + I + G model (lnL = 7,632.2964; freqA = 0.1794; freqC = 0.2463; freqG = 0.3573; freqT = 0.2170; rAC = 0.9583; rAG = 2.6626; rAT = 1.2424; rCG = 0.6711; rCT = 5.4981; Pinv = 0.2528; Alpha = 0.6707). Bayesian posterior probability (BPP) values >0.50 are given for appropriate clades. The sequence of the new species is indicated by bold font.

Morphometric measurements of Cephalenchus driekieae n_ sp_ from South Africa_

Female
CharactersHolotypeParatypes
n18
L456483.5 ± 24.5 (451–526)
a36.534.8 ± 1.3 (33.3–36.5)
b77.3 ± 0.5 (6.8–8.0)
c44.3 ± 0.1 (4.1–4.5)
12.213.6 ± 1.3 (11.5–15.0)
V65.864.8 ± 2.0 (60.5–67.0)
86.583.0 ± 3.3 (75.5–86.5)
Lip region height22.4 ± 0.4 (2–3)
Lip region width5.56.0 ± 0.5 (5.5–7.0)
Stylet conus length55.4 ± 0.6 (4.4–6.3)
Stylet length1212.0 ± 0.5 (11.5–13.0)
DGO1.81.3 ± 0.3 (1.0–1.8)
Median bulb valve to ant. end3131.0 ± 0.5 (30–32)
MB%6.86.5 ± 0.3 (6–7)
Pharynx (stylet knobs to pharynx-intestinal junction)5664.5 ± 5.0 (56–71)
Neck (anterior end to pharynx-intestinal junction)7065 ± 3 (61–70)
Excretory pore – ant. end61.5 ± 3.0 (57–65)
Hemizonid – ant. end.4958 ± 5 (49–62)
Anterior end to vulva300313 ± 22 (280–353)
 – to anus346372 ± 20 (344–393)
Body width at median bulb1112.0 ± 0.5 (11–13)
 – at vulva12.514 ± 1 (12.5–15.5)
 – at anus98.5 ± 1.0 (7.5–10.0)
Post-vulval uterine sac length1313.5 ± 0.5 (13–14)
Tail length110111.0 ± 3.5 (105–116)
DOI: https://doi.org/10.21307/jofnem-2020-031 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
Language: English
Page range: 1 - 10
Submitted on: Jan 30, 2020
Published on: Apr 24, 2020
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year

© 2020 Milad Rashidifard, Gerhard Du Preez, Joaquín Abolafia, Majid Pedram, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.