Figure 1:
Figure 2:
Figure 3:
Figure 4:
Nematode species, locality, associated host and sequences used in this study_
| Species | Locality | Host-plant | Accession number |
|---|---|---|---|
| Enchodelus cf longispiculus | Gorgan province, Iran | – | KP190119 |
| Enchodelus sp | Hamedan province, Iran | – | KP190120 |
| Enchodelus sp | – | – | EF207240 |
| Enchodelus macrodorus | – | – | AY593054 |
| Enchodeloides signyensis | KY881719 | ||
| Enchodorus dolichurus | – | – | KR184124 |
| Enchodorus dolichurus | – | – | KR184125 |
| Enchodorus yeatsi | Andimeshk, Khuzestan province, Iran | Mosses in a natural region | KX691911 |
| Heterodorus youbertghostai | Sabalan mountains, Iran | Grasslands | KR184127 |
| Heterodorus youbertghostai | Arasbaran forests, Kaleybar, East-Azarbayjan province, Iran | Grasses | KR184126 |
| Heterodorus brevidentatus | Kerman, Iran | – | KP963962 |
| Longidorella penetrans | – | – | HM235515 |
| Longidorella cf macramphis | – | – | AY593042 |
| Paravulvus hartingii | – | – | AY593062 |
| Pungentus silvestris | – | – | AY593052 |
| Pungentus silvestris | – | – | AY593053 |
| Pungentus engadinensis | – | – | AY593050 |
| Pungentus engadinensis | Damghan, Semnan province, Iran | Fruit trees | MH346473 |
| Pungentus engadinensis | Noshahr, Mazandaran province, Iran | Forest trees | MH346474 |
| Pungentus monohystera | Germany | Sediment | MF325343 |
| Pungentus monohystera | Germany | Sediment | MF325344 |
| Pungentus azarbaijanensis | West-Azarbaijan province, Iran | Grasses | MH346476 |
| Pungentus azarbaijanensis | West-Azarbaijan province, Iran | Grasses | MH346477 |
| Pungentus sufiyanensis n. sp. | Sufiyan, East-Azarbaijan province, Iran | Black cherry trees (Prunus cerasus L.) | MN855359 |
| Rhyssocolpus vinciguerrae | Astara forests, north-western Iran | Forest trees | KP204547 |
Morphometric data for Pungentus sufiyanensis n_ sp_
| Sufiyan population | ||||
|---|---|---|---|---|
| Locality | Female | Bokan population | Marand population | |
| Characters | Holotype | Paratypes | Female | Female |
| n | – | 7 | 8 | 2 |
| L | 1.49 | 1.53 ± 0.08 | 1.41 ± 0.09 | 1.27 ± 0.07 |
| (1.40–1.57) | (1.33–1.54) | (1.22–1.33) | ||
| a | 45 | 42.0 ± 3.3 | 47.0 ± 2.8 | 46.0 ± 2.3 |
| (40.0–47.0) | (42.0–50.0) | (43.0–49.0) | ||
| b | 4.4 | 4.5 ± 0.1 | 4.4 ± 0.3 | 3.9 ± 0.7 |
| (4.3–4.8) | (4.0–5.0) | (3.9–4.0) | ||
| c | 68 | 75.0 ± 6.1 | 70.0 ± 4.7 | 68.0 ± 1.4 |
| (65.0–84.0) | (66.0–75.0) | (67.0–70.0) | ||
| c´ | 1 | 0.8 ± 0.01 | 0.9 ± 0.07 | 0.9 ± 0.01 |
| (0.7–1.0) | (0.8–1.0) | (0.8–1.0) | ||
| V | 50 | 49.0 ± 1.0 | 49.0 ± 1.0 | 51.0 ± 2.3 |
| (47.0–50.0) | (48.0–51.0) | (49.0–54.0) | ||
| Lip region diam. | 8 | 8.4 ± 0.3 | 8.2 ± 0.6 | 8.0 ± 0.5 |
| (8.0–9.0) | (7.5–9.0) | (7.0–9.0) | ||
| Odontostyle length | 20 | 19.0 ± 0.7 | 19.0 ± 1.4 | 20.0 ± 0.4 |
| (18.0–20.0) | (18.0–20.5) | (19.0–21.0) | ||
| Odontophore length | 18 | 17.0 ± 0.8 | 16.5 ± 0.2 | 17.0 ± 1.8 |
| (15.0–18.0) | (16.0–17.0) | (16.0–19.0) | ||
| Guiding ring from ant. end | 13 | 13.0 ± 0.5 | 13.5 ± 0.0 | 14.0 ± 0.0 |
| (12.0–14.0) | (13.5) | (14.0) | ||
| Neck length | 325 | 335 ± 7 | 340 ± 15 | 315 ± 10 |
| (325–348) | (304–356) | (309–328) | ||
| Phar. expansion length | 148 | 150.0 ± 2.8 | 149.0 ± 5.2 | 138.0 ± 4.7 |
| (147.0–155.0) | (138.0–161.0) | (133.0–144.0) | ||
| Body diam. at neck base | 30 | 33.0 ± 1.9 | 29.0 ± 1.8 | 26.0 ± 0.0 |
| mid-body anus | (30.0–38.0) | (27.0–31.0) | (26.0) | |
| 32 | 35.0 ± 1.6 | 29.0 ± 0.4 | 27.5 ± 0.3 | |
| (32.0–38.0) | (28.0–30.0) | (27.0–28.0) | ||
| 20 | 23.0 ± 2.6 | 19.5 ± 1.1 | 20.0 ± 0.6 | |
| (20.0–26.0) | (18.0–21.0) | (19.0–21.0) | ||
| Prerectum length | 75 | 71 ± 11 | 67.0 ± 3.1 | 83.0 ± 2.0 |
| (57–90) | (62.0–73.0) | (82.0–85.0) | ||
| Rectum length | 18 | 19.0 ± 1.8 | 20.0 ± 1.2 | 20.5 ± 0.4 |
| (18.0–23.0) | (19.0–22.0) | (20.0–21.0) | ||
| Tail length | 21 | 20.0 ± 1.8 | 19.0 ± 2.0 | 18.5 ± 0.2 |
| (18.0–23.0) | (17.5–23.0) | (18.0–19.0) | ||
Morphometric data for six Iranian populations of Pungentus engadinensis_
| Locality | Urmia population | Divandarreh population | Bokan population | Maragheh population | Sufiyan population | Basmenj population |
|---|---|---|---|---|---|---|
| Characters | Female | Female | Female | Female | Female | Female |
| n | 5 | 6 | 7 | 7 | 5 | 6 |
| L | 0.90 ± 0.06 | 1.10 ± 0.01 | 0.99 ± 0.07 | 1.00 ± 0.05 | 0.95 ± 0.03 | 0.99 ± 0.03 |
| (0.83–1.00) | (0.91–1.22) | (0.90–1.10) | (0.90–1.10) | (0.90–0.99) | (0.96–1.06) | |
| a | 39.0 ± 2.4 | 40.5 ± 0.5 | 39.0 ± 2.3 | 35.5 ± 1.4 | 38.0 ± 1.9 | 34.8 ± 2.0 |
| (36.5–42.0) | (36.5–50.5) | (34.5–41.5) | (34.0–37.0) | (36.0–41.0) | (35.0–37.0) | |
| b | 3.7 ± 0.2 | 4.0 ± 0.6 | 3.9 ± 0.2 | 4.0 ± 0.2 | 4.0 ± 0.1 | 3.8 ± 0.2 |
| (3.5–4.5) | (3.8–5.0) | (3.5–4.0) | (4.0–4.5) | (3.9–4.2) | (3.5–4.2) | |
| c | 47.0 ± 4.6 | 60.5 ± 5.5 | 52.0 ± 0.5 | 60.5 ± 6.4 | 54.0 ± 3.4 | 58.6 ± 7.7 |
| (42.0–52.5) | (55.0–68.0) | (44.5–59.0) | (52.0–72.0) | (50.0–59.0) | (46.0–68.0) | |
| c´ | 1.1 ± 0.08 | 0.9 ± 0.09 | 1.0 ± 0.01 | 0.0 ± 0.06 | 0.94 ± 0.05 | 0.81 ± 0.07 |
| (1.0–1.2) | (0.7–1.1) | (0.9–1.1) | (0.8–0.9) | (0.9–1.0) | (0.7–0.9) | |
| V | 48.0 ± 2.5 | 45.5 ± 0.8 | 46.0 ± 1.4 | 46.0 ± 1.3 | 47.0 ± 1.6 | 44.3 ± 3.2 |
| (44.0–52.0) | (44.5–47.0) | (44.0–48.0) | (44.0–47.0) | (45.0–49.0) | (41.0–49.0) | |
| Lip region diam. | 8.3 ± 0.5 | 10.6 ± 0.2 | 10.0 ± 0.6 | 10.0 ± 0.5 | 8.6 ± 0.4 | 8.8 ± 0.4 |
| (8.0–9.0) | (10.0–11.0) | (8.0–11.0) | (9.0–11.0) | (8.0–9.0) | (8.0–9.0) | |
| Odontostyle length | 15.0 ± 0.5 | 18.0 ± 0.5 | 15.3 ± 0.1 | 17.5 ± 0.2 | 16.0 ± 0.0 | 15.9 ± 0.9 |
| (14.5–16.0) | (17.5–18.5) | (14.0–17.0) | (16.0–18.0) | (16.0) | (15–17.5) | |
| Odontophore length | 19.5 ± 0.6 | 15.6 ± 2.1 | 16.6 ± 1.8 | 20.8 ± 1.3 | 14.2 ± 0.6 | 19.6 ± 0.9 |
| (19.0–20.5) | (13.0–18.5) | (14.1–19.3) | (19.0–22.0) | (13.0–15.0) | (17.0–21.0) | |
| Guiding ring from ant. end | 10.3 ± 0.5 | 11.2 ± 0.4 | 10.6 ± 0.8 | 10.3 ± 0.4 | 10.2 ± 0.4 | 11.3 ± 0.4 |
| (10.0–11.4) | (11.0–12.0) | (9.0–12.0) | (10.0–11.0) | (9.0–11.0) | (9.6–12.0) | |
| Neck length | 238 ± 13 | 265 ± 14 | 259 ± 21 | 302 ± 13 | 236 ± 11 | 254 ± 10 |
| (223–255) | (233–295) | (228–293) | (228–329) | (219–248) | (243–266) | |
| Phar. expansion length | 91.2 ± 8.3 | 104.2 ± 3.1 | 89.6 ± 9.7 | 108.2 ± 4.4 | 96.0 ± 9.1 | 101.0 ± 5.2 |
| (84.0–105.0) | (102.0–108.0) | (81.0–106.0) | (101.0–116.0) | (85.0–108.0) | (95.0–109.0) | |
| Body diam. at neck base | 26.2 ± 0.4 | 29.6 ± 2.7 | 22.2 ± 1.5 | 26.0 ± 0.7 | 23.0 ± 0.7 | 26.0 ± 0.9 |
| (25.0–27.0) | (25.0–34.0) | (21.0–24.1) | (25.0–27.0) | (22.0–24.0) | (25.0–27.0) | |
| mid-body | 27.4 ± 0.3 | 32.2 ± 2.0 | 23.3 ± 0.8 | 28.3 ± 0.4 | 24.8 ± 0.1 | 28.2 ± 1.3 |
| (27.0–28.2) | (28.0–37.0) | (22.0–25.0) | (27.0–29.1) | (24.0–25.0) | (27.0–30.0) | |
| anus | 18.3 ± 0.5 | 22.3 ± 2.3 | 18.1 ± 0.6 | 20.0 ± 0.7 | 18.0 ± 0.7 | 20.0 ± 1.7 |
| (17.0–19.0) | (20.0–25.0) | (17.0–19.0) | (19.0–21.0) | (17.0–19.0) | (19.0–22.0) | |
| Prerectum length | 72.6 ± 13.2 | 85.1 ± 12.4 | 55.2 ± 7.7 | 82.2 ± 2.2 | 40.0 ± 6.8 | 64.3 ± 5.1 |
| (52.0–94.0) | (72.0–101.0) | (50.3–64.0) | (77.0–88.0) | (30.2–50.0) | (59.0–69.0) | |
| Rectum length | 17.6 ± 0.2 | 20.3 ± 1.5 | 14.3 ± 0.5 | 18.1 ± 1.2 | 16.0 ± 2.4 | 22.0 ± 2.2 |
| (16.0–18.0) | (18.0–22.0) | (13.0–16.0) | (16.0–20.0) | (14.0–20.0) | (19.0–25.0) | |
| Tail length | 18.5 ± 0.8 | 17.5 ± 0.9 | 19.0 ± 0.7 | 16.7 ± 1.2 | 18.0 ± 0.8 | 17.1 ± 2.4 |
| (17.5–22.5) | (16.5–21.0) | (18.0–20.0) | (14.0–18.0) | (17.0–19.0) | (15.0–21.0) |