Table 1.
Morphometric data for Nothocriconemoides hangzhouensis n. sp.
| Holotype | Paratype | |
|---|---|---|
| n | 17 | |
| Body Length | 494.0 | 487.1±43.8 (419.6-572.3) |
| R | 36.0 | 37.2±1.2 (35.0-39.0) |
| Rst | 7.0 | 6.5±0.5 (6.0-7.0) |
| Rex | 15.0 | 14.7±0.6 (13.0-15.0) |
| RV | 3.0 | 2.9±0.2 (2.0-3.0) |
| Rvan | 0.0 | 0.0±0.0 (0.0-0.0) |
| Ran | 3.0 | 2.9±0.2 (2.0-3.0) |
| a | 8.2 | 7.9±0.7 (6.3-9.4) |
| b | 4.2 | 4.1±0.3 (3.5-4.5) |
| c | 18.6 | 17.3±1.7 (14.2-20.0) |
| c' | 0.8 | 0.9±0.1 (0.7-1.1) |
| V | 93.2 | 92.6±0.9 (90.5-94.0) |
| VL/VB | 0.9 | 1.0±0.1 (0.9-1.2) |
| Lip height | 8.8 | 9.5±0.7 (7.8-10.6) |
| Lip diam. | 18.4 | 20.2±1.4 (17.2-22.1) |
| Stylet length | 71.0 | 71.1±3.0 (64.4-75.5) |
| Stylet percentage | 14.4 | 14.7±1.2 (13.1-17.4) |
| Pharynx length | 117.5 | 118.9±5.0 (111.8-129.6) |
| Body width | 60.3 | 61.8±5.3 (52.0-69. 4) |
| Vulval body diam. | 37.0 | 35.7±2.5 (31.8-38.5) |
| Anal body diam. | 31.9 | 32.1±3.1 (26.4-37.4) |
| Vulva to tail terminus | 33.4 | 36.0±4.0 (29.8-41.7) |
| Tail length | 26.5 | 28.3±1.9 (23.3-30.5) |
| Annuli width | 13.1 | 14.5±1.2 (13.1-16.9) |
Figure 1:
Line drawings of Nothocriconemoides hangzhouensis n. sp. Female A: esophageal region; B: Cepahlic region; C: En face view: D-F: Cuticle markings; G: Tail region under SEM; H: Tail region under LM; I: Cepahlic region of juvenile: J: Crenation on cuticle of juvenile; K: Tail region of juvenile. (Scale bars = A =50 μm, B-I = 10 μm).
Figure 2:
Light photomicrographs of Nothocriconemoides hangzhouensis n. sp. Female A: Entire body; B-E: Cepahlic regions; F-H: Esophageal regions, arrow pointing on the excretory pore (exp): I-K: Cuticle markings; L-O: Tail regions, arrows pointing on vulva (v) and anus (a). (Scale bars=A=50 μm, B-I=10 μm).
Figure 3:
Light photomicrographs of Nothocriconemoides hangzhouensis n. sp. Juvenile A: Entire body; B: Cepahlic region; C,D: Esophageal regions: E: Crenation on cuticle; F, G: Tail regions, arrows pointing on anus (a). (Scale bars=A=50 μm, B-I=10 μm).
Figure 4:
Scanning electron microscopy of Nothocriconemoides hangzhouensis n. sp. Female. A: Entire body; B-D: En face view; E: Cuticle markings; F-H: tail regions arrows pointing on vulva (v) and anus (a) (Scale bars, A = 100 μm; B, C = 10 μm; D, H = 20 μm; G-F = 30 μm).
Figure 5:
Phylogenetic relationships of Nothocriconemoides hangzhouensis n. sp. with other criconematids species as inferred from Bayesian analysis using the 18 S rRNA gene sequence dataset with the GTR + I + G model (−lnL=7,315.8130; AIC = 14,859.6260; freqA=0.2371; freqC=0.2413; freqG=0.2833; freqT=0.2384; R(a)=1.5166; R(b)=2.2509; R(c)=09364; R(d)=0.7246; R(e)=6.0997; R(f)=1.0000; Pinva=0.6630; and Shape=0.6070). Posterior probability more than 70% is given for appropriate clades. Newly obtained sequences are indicated in bold.
Figure 6:
Phylogenetic relationships of Nothocriconemoides hangzhouensis n. sp. with other criconematids species as inferred from Bayesian analysis using the D2-D3 of 28 S rRNA gene sequence dataset with the GTR + I + G model (−lnL=8,382.1334; AIC=16,972.2669; freqA=0.1451; freqC=0.2354; freqG=0.3515; freqT=0.2681; R(a)=0.8404; R(b)=2.5613; R(c)=1.6924; R(d)=0.4616; R(e)=4.7092; R(f)=1.0000; Pinva=0.2730; and Shape=0.8370). Posterior probability more than 70% is given for appropriate clades. Newly obtained sequences are indicated in bold.
Figure 7:
Phylogenetic relationships of Nothocriconemoides hangzhouensis n. sp. with other criconematids species as inferred from Bayesian analysis using the ITS rRNA gene sequence dataset with the GTR + I + G model (−lnL=7727.9982; AIC = 15603.9963; freqA=0.2067; freqC=0.2560; freqG=0.2814; freqT=0.2559; R(a)=1.5781; R(b)=2.9918; R(c)=1.7856; R(d)=0.6423; R(e)=2.8799; R(f)=1.0000; Pinva=0.0460; and Shape=0.6180). Posterior probability more than 70% is given for appropriate clades. Newly obtained sequences are indicated in bold.
Figure 8:
Phylogenetic relationships of Nothocriconemoides hangzhouensis n. sp. with other criconematids species as inferred from Bayesian analysis using the coxI gene sequence dataset with the GTR + I + G model (−lnL=13,473.0592; AIC=27,198.1184; freqA=0.3715; freqC=0.0509; freqG=0.0477; freqT=0.5299; R(a)=0.7544; R(b)=36.5547; R(c)=1.6680; R(d)=51.5187; R(e)=20.3538; R(f)=1.0000; Pinva=0.2510; and Shape=0.3470). Posterior probability more than 70% is given for appropriate clades. Newly obtained sequences are indicated in bold.