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A new rare nematode Nothocriconemoides hangzhouensis n. sp. (Nematoda: Criconematidae) from Hangzhou, China

Journal of Nematology
Volume 52 (2020): Issue 1 (January 2020)
By:
Open Access
|Mar 2020

Figures & Tables

Figure 1:

Line drawings of Nothocriconemoides hangzhouensis n. sp. Female A: esophageal region; B: Cepahlic region; C: En face view: D-F: Cuticle markings; G: Tail region under SEM; H: Tail region under LM; I: Cepahlic region of juvenile: J: Crenation on cuticle of juvenile; K: Tail region of juvenile. (Scale bars = A =50 μm, B-I = 10 μm).
Line drawings of Nothocriconemoides hangzhouensis n. sp. Female A: esophageal region; B: Cepahlic region; C: En face view: D-F: Cuticle markings; G: Tail region under SEM; H: Tail region under LM; I: Cepahlic region of juvenile: J: Crenation on cuticle of juvenile; K: Tail region of juvenile. (Scale bars = A =50 μm, B-I = 10 μm).

Figure 2:

Light photomicrographs of Nothocriconemoides hangzhouensis n. sp. Female A: Entire body; B-E: Cepahlic regions; F-H: Esophageal regions, arrow pointing on the excretory pore (exp): I-K: Cuticle markings; L-O: Tail regions, arrows pointing on vulva (v) and anus (a). (Scale bars=A=50 μm, B-I=10 μm).
Light photomicrographs of Nothocriconemoides hangzhouensis n. sp. Female A: Entire body; B-E: Cepahlic regions; F-H: Esophageal regions, arrow pointing on the excretory pore (exp): I-K: Cuticle markings; L-O: Tail regions, arrows pointing on vulva (v) and anus (a). (Scale bars=A=50 μm, B-I=10 μm).

Figure 3:

Light photomicrographs of Nothocriconemoides hangzhouensis n. sp. Juvenile A: Entire body; B: Cepahlic region; C,D: Esophageal regions: E: Crenation on cuticle; F, G: Tail regions, arrows pointing on anus (a). (Scale bars=A=50 μm, B-I=10 μm).
Light photomicrographs of Nothocriconemoides hangzhouensis n. sp. Juvenile A: Entire body; B: Cepahlic region; C,D: Esophageal regions: E: Crenation on cuticle; F, G: Tail regions, arrows pointing on anus (a). (Scale bars=A=50 μm, B-I=10 μm).

Figure 4:

Scanning electron microscopy of Nothocriconemoides hangzhouensis n. sp. Female. A: Entire body; B-D: En face view; E: Cuticle markings; F-H: tail regions arrows pointing on vulva (v) and anus (a) (Scale bars, A = 100 μm; B, C = 10 μm; D, H = 20 μm; G-F = 30 μm).
Scanning electron microscopy of Nothocriconemoides hangzhouensis n. sp. Female. A: Entire body; B-D: En face view; E: Cuticle markings; F-H: tail regions arrows pointing on vulva (v) and anus (a) (Scale bars, A = 100 μm; B, C = 10 μm; D, H = 20 μm; G-F = 30 μm).

Figure 5:

Phylogenetic relationships of Nothocriconemoides hangzhouensis n. sp. with other criconematids species as inferred from Bayesian analysis using the 18 S rRNA gene sequence dataset with the GTR + I + G model (−lnL=7,315.8130; AIC = 14,859.6260; freqA=0.2371; freqC=0.2413; freqG=0.2833; freqT=0.2384; R(a)=1.5166; R(b)=2.2509; R(c)=09364; R(d)=0.7246; R(e)=6.0997; R(f)=1.0000; Pinva=0.6630; and Shape=0.6070). Posterior probability more than 70% is given for appropriate clades. Newly obtained sequences are indicated in bold.
Phylogenetic relationships of Nothocriconemoides hangzhouensis n. sp. with other criconematids species as inferred from Bayesian analysis using the 18 S rRNA gene sequence dataset with the GTR + I + G model (−lnL=7,315.8130; AIC = 14,859.6260; freqA=0.2371; freqC=0.2413; freqG=0.2833; freqT=0.2384; R(a)=1.5166; R(b)=2.2509; R(c)=09364; R(d)=0.7246; R(e)=6.0997; R(f)=1.0000; Pinva=0.6630; and Shape=0.6070). Posterior probability more than 70% is given for appropriate clades. Newly obtained sequences are indicated in bold.

Figure 6:

Phylogenetic relationships of Nothocriconemoides hangzhouensis n. sp. with other criconematids species as inferred from Bayesian analysis using the D2-D3 of 28 S rRNA gene sequence dataset with the GTR + I + G model (−lnL=8,382.1334; AIC=16,972.2669; freqA=0.1451; freqC=0.2354; freqG=0.3515; freqT=0.2681; R(a)=0.8404; R(b)=2.5613; R(c)=1.6924; R(d)=0.4616; R(e)=4.7092; R(f)=1.0000; Pinva=0.2730; and Shape=0.8370). Posterior probability more than 70% is given for appropriate clades. Newly obtained sequences are indicated in bold.
Phylogenetic relationships of Nothocriconemoides hangzhouensis n. sp. with other criconematids species as inferred from Bayesian analysis using the D2-D3 of 28 S rRNA gene sequence dataset with the GTR + I + G model (−lnL=8,382.1334; AIC=16,972.2669; freqA=0.1451; freqC=0.2354; freqG=0.3515; freqT=0.2681; R(a)=0.8404; R(b)=2.5613; R(c)=1.6924; R(d)=0.4616; R(e)=4.7092; R(f)=1.0000; Pinva=0.2730; and Shape=0.8370). Posterior probability more than 70% is given for appropriate clades. Newly obtained sequences are indicated in bold.

Figure 7:

Phylogenetic relationships of Nothocriconemoides hangzhouensis n. sp. with other criconematids species as inferred from Bayesian analysis using the ITS rRNA gene sequence dataset with the GTR + I + G model (−lnL=7727.9982; AIC = 15603.9963; freqA=0.2067; freqC=0.2560; freqG=0.2814; freqT=0.2559; R(a)=1.5781; R(b)=2.9918; R(c)=1.7856; R(d)=0.6423; R(e)=2.8799; R(f)=1.0000; Pinva=0.0460; and Shape=0.6180). Posterior probability more than 70% is given for appropriate clades. Newly obtained sequences are indicated in bold.
Phylogenetic relationships of Nothocriconemoides hangzhouensis n. sp. with other criconematids species as inferred from Bayesian analysis using the ITS rRNA gene sequence dataset with the GTR + I + G model (−lnL=7727.9982; AIC = 15603.9963; freqA=0.2067; freqC=0.2560; freqG=0.2814; freqT=0.2559; R(a)=1.5781; R(b)=2.9918; R(c)=1.7856; R(d)=0.6423; R(e)=2.8799; R(f)=1.0000; Pinva=0.0460; and Shape=0.6180). Posterior probability more than 70% is given for appropriate clades. Newly obtained sequences are indicated in bold.

Figure 8:

Phylogenetic relationships of Nothocriconemoides hangzhouensis n. sp. with other criconematids species as inferred from Bayesian analysis using the coxI gene sequence dataset with the GTR + I + G model (−lnL=13,473.0592; AIC=27,198.1184; freqA=0.3715; freqC=0.0509; freqG=0.0477; freqT=0.5299; R(a)=0.7544; R(b)=36.5547; R(c)=1.6680; R(d)=51.5187; R(e)=20.3538; R(f)=1.0000; Pinva=0.2510; and Shape=0.3470). Posterior probability more than 70% is given for appropriate clades. Newly obtained sequences are indicated in bold.
Phylogenetic relationships of Nothocriconemoides hangzhouensis n. sp. with other criconematids species as inferred from Bayesian analysis using the coxI gene sequence dataset with the GTR + I + G model (−lnL=13,473.0592; AIC=27,198.1184; freqA=0.3715; freqC=0.0509; freqG=0.0477; freqT=0.5299; R(a)=0.7544; R(b)=36.5547; R(c)=1.6680; R(d)=51.5187; R(e)=20.3538; R(f)=1.0000; Pinva=0.2510; and Shape=0.3470). Posterior probability more than 70% is given for appropriate clades. Newly obtained sequences are indicated in bold.

Morphometric data for Nothocriconemoides hangzhouensis n_ sp_

HolotypeParatype
n 17
Body Length494.0487.1±43.8 (419.6-572.3)
R36.037.2±1.2 (35.0-39.0)
Rst7.06.5±0.5 (6.0-7.0)
Rex15.014.7±0.6 (13.0-15.0)
RV3.02.9±0.2 (2.0-3.0)
Rvan0.00.0±0.0 (0.0-0.0)
Ran3.02.9±0.2 (2.0-3.0)
a8.27.9±0.7 (6.3-9.4)
b4.24.1±0.3 (3.5-4.5)
c18.617.3±1.7 (14.2-20.0)
c'0.80.9±0.1 (0.7-1.1)
V93.292.6±0.9 (90.5-94.0)
VL/VB0.91.0±0.1 (0.9-1.2)
Lip height8.89.5±0.7 (7.8-10.6)
Lip diam.18.420.2±1.4 (17.2-22.1)
Stylet length71.071.1±3.0 (64.4-75.5)
Stylet percentage14.414.7±1.2 (13.1-17.4)
Pharynx length117.5118.9±5.0 (111.8-129.6)
Body width60.361.8±5.3 (52.0-69. 4)
Vulval body diam.37.035.7±2.5 (31.8-38.5)
Anal body diam.31.932.1±3.1 (26.4-37.4)
Vulva to tail terminus33.436.0±4.0 (29.8-41.7)
Tail length26.528.3±1.9 (23.3-30.5)
Annuli width13.114.5±1.2 (13.1-16.9)
DOI: https://doi.org/10.21307/jofnem-2020-006 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
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Language: English
Page range: 1 - 14
Submitted on: Jan 6, 2020
Published on: Mar 18, 2020
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year
Keywords:
DNA sequencing,
Elm tree,
Morphology,
Morphometrics,
Nematode,
New record,
Species,
Phylogeny,
Scanning electron microscopy.
Related subjects:
Life sciences,
Life sciences, other

© 2020 Munawar Maria, Wentao Miao, Ruihang Cai, Pablo Castillo, Jingwu Zheng, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.

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