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A draft genome of a field-collected Steinernema feltiae strain NW

Open Access
|Mar 2020

Figures & Tables

Figure 1:

Genome alignment of strains SN and NW. Only top 100 longest scaffolds from SN (laid across the x-axis) and top 100 longest contigs from NW (y-axis) were shown here to minimize noise. Each contig/scaffold is shown between two lines (vertical for SN and horizontal for NW) along the axes. A colored dot is plotted wherever the two sequences agree; the forward matches are shown in purple, while the reverse matches are shown in blue. If the two genomes were perfectly identical, a series of purple dots would be drawn diagonally.
Genome alignment of strains SN and NW. Only top 100 longest scaffolds from SN (laid across the x-axis) and top 100 longest contigs from NW (y-axis) were shown here to minimize noise. Each contig/scaffold is shown between two lines (vertical for SN and horizontal for NW) along the axes. A colored dot is plotted wherever the two sequences agree; the forward matches are shown in purple, while the reverse matches are shown in blue. If the two genomes were perfectly identical, a series of purple dots would be drawn diagonally.

Summary of the genome assembly of strain NW in comparison to strain SN_

Strain NWStrain SN
Assembly
Total bases (bp)121,603,26082,627,153
No. of scaffoldsna5,839
Scaffold N50na47,851
No. of contigs4,67859,024
Contig N5060,4333,650
No. of gaps (bp)02,769,616
Longest scaffolds (bp)1,315,9811,446,875
No. of predicted genes32,30436,434
Total no. of amino acids12,632,60112,195,137
BUSCO assessment
Complete BUSCOs87.27%84.32%
Complete single copy76.68%80.55%
Complete duplicated copy10.59%3.77%
Fragmented7.33%8.15%
Missing5.40%7.54%
Alignment
Aligned Seqs4,279 (91.47%)5,418 (92.79%)
Aligned Bases92,999,444 (76.48%)74,444,081 (90.10%)
1-to-149,94949,949
Total length (1-to-1)72,468,26972,538,432
Avg. identity (1-to-1)97.0797.07
M-to-M133,667133,667
Total length (M-to-M)106,859,791106,997,101
Avg. identity (M-to-M)95.6995.69
Genomic variation
Breakpoints261,902252,282
Relocations8,4565,231
Translocations13,59516,494
Inversions1,4111,952
Insertions105,32574,670
Total SNPs907,706907,706
DOI: https://doi.org/10.21307/jofnem-2020-003 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
Language: English
Page range: 1 - 7
Submitted on: Jul 23, 2019
Published on: Mar 17, 2020
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year

© 2020 Zhen Fu, Yuxiang Li, Axel A. Elling, William E. Snyder, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.