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Figure A3:
Annotated genes differentially expressed in response to punicalagin and related to physiological processes in Bursaphelenchus xylophilus_
| TRINITY_Gene ID | Annotation | Log2 FC | FDR | Type |
|---|---|---|---|---|
| DN8346_c0_g1 | Cytoplasmic dynein heavy chain | 3.0314 | 2.54E-24 | Up |
| DN18432_c0_g1 | ATP synthase F0 subunit 6 (mitochondrion) | −3.5432 | 8.44E-20 | Down |
| DN6713_c0_g1 | Twitchin | 2.6719 | 1.19E-18 | Up |
| DN2658_c0_g1 | Cytochrome c oxidase subunit I (mitochondrion) | −2.2625 | 1.66E-12 | Down |
| DN11917_c0_g2 | Cytochrome b, partial (mitochondrion) | −2.3332 | 3.83E-09 | Down |
| DN9703_c0_g1 | Cytochrome c oxidase subunit 3 (mitochondrion) | -2.2922 | 3.88E-09 | Down |
| DN1325_c0_g2 | Heat shock protein 20 | −2.3758 | 1.70E-05 | Down |
| DN14208_c0_g1 | Small HSP21-like protein | −1.4702 | 6.73E-05 | Down |
| DN14130_c0_g1 | Heat shock protein Hsp-12.2 | −1.3353 | 0.0005945 | Down |
| DN10040_c0_g1 | Electron-transfer-flavoprotein | −1.1439 | 0.01728 | Down |
| DN8075_c0_g1 | Nematode cuticle collagen and collagen triple helix repeat domain containing protein | −1.1863 | 0.01908 | Down |
| DN14349_c1_g1 | Glucosidase 2 subunit beta | −1.0567 | 0.02580 | Down |
| DN22_c0_g1 | NADH dehydrogenase subunit 1 (mitochondrion) | 2.2809 | 0.04550 | Up |
Data statistics of raw reads from six samples for RNAseq_
| Sample | Raw reads | Raw reads base (bp) | Q20 (%) | Q30 (%) |
|---|---|---|---|---|
| CK1 | 31,003,104 | 4,650,465,600 | 95.72 | 92.01 |
| CK2 | 37,538,058 | 5,630,708,700 | 95.1 | 91.11 |
| CK3 | 37,726,236 | 5,658,935,400 | 95.31 | 90.95 |
| P1 | 35,871,190 | 5,380,678,500 | 96.92 | 93.53 |
| P2 | 46,131,806 | 6,919,770,900 | 96.48 | 92.26 |
| P3 | 33,488,164 | 5,023,224,600 | 97.32 | 93.81 |
Primer sequences used for qRT-PCR validation of differentially expressed genes_
| Gene ID | Primer sequence | Amplicon length (bp) |
|---|---|---|
| DN8346_c0_g1 | Forward: CTGCTGAATGAGTGGGTA | 197 |
| Reverse: AGAAGTTTGAAAGGAGGC | ||
| DN18432_c0_g1 | Forward: AAGTGTCACCTCCTTTAC | 132 |
| Reverse: GGTATTCGTTTTGTCCT | ||
| DN6713_c0_g1 | Forward: CGAGGTCCGTTAGAAGTG | 128 |
| Reverse: TTGCCAGTCTCAGTGTCC | ||
| DN14208_c0_g1 | Forward: GTAAGCCTGGAGAAAAG | 195 |
| Reverse: AGTTGACGGTGTTGGTG | ||
| DN8075_c0_g1 | Forward: GCAACCACCAGGAGCAAC | 88 |
| Reverse: CGGAAATGATGGAGAACCC | ||
| DN14349_c1_g1 | Forward: AAGTGACGAGCCAGGTA | 168 |
| Reverse: TCACAAACATTCGGACA | ||
| DN6594_c0_g1a | Forward: CAACCCCAAGGCTAACA | 303 |
| Reverse: TCACGCACGATTTCACG |
Data statistics of clean reads from six samples after quality control_
| Sample | Clean reads | Clean reads base (bp) | Q20 (%) | Q30 (%) |
|---|---|---|---|---|
| CK1 | 28,585,924 | 4,136,450,402 | 98.48 | 95.62 |
| CK2 | 34,085,674 | 4,903,647,270 | 98.36 | 95.36 |
| CK3 | 34,177,082 | 4,955,215,491 | 98.19 | 94.97 |
| P1 | 33,854,004 | 4,938,319,926 | 98.6 | 95.91 |
| P2 | 43,447,298 | 6,287,507,779 | 98.11 | 94.77 |
| P3 | 32,165,338 | 4,669,426,049 | 98.45 | 95.57 |