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Description of Hirschmanniella dicksoni n. sp. (Nematoda: Pratylenchidae) from rhizosphere soil of limpograss from Florida, USA Cover

Description of Hirschmanniella dicksoni n. sp. (Nematoda: Pratylenchidae) from rhizosphere soil of limpograss from Florida, USA

Open Access
|Dec 2019

Figures & Tables

Figure 1:

Hirschmanniella dicksoni n. sp. female: (A) Anterior region; (B) tail region (arrow 1: subterminal notch, arrow 2: ventral projection); (C) tail region. Scale bars in A, B, C = 20 µm.

Figure 2:

Hirschmanniella dicksoni n. sp. male: (A) Anterior region; (B, C, D) tail region (arrows: pointed and round tail terminus). Scale bars in A, B, C, D = 20 µm.

Figure 3:

SEM photomicrographs of Hirschmanniella dicksoni n. sp. female: (A) Lip region (arrow: incomplete lip annules); (B) lateral field in anterior region (arrows: incomplete annules; rings and incisures on the beginning on lateral field); (C) posterior region; (D) vulva and lateral fields; (E) lateral field in middle body region showing incomplete aerolations; (F) tail region with subterminal notch (arrow: subterminal notch).

Figure 4:

Phylogenetic relationship based on D2–D3 expansion segments of 28S rRNA gene sequences within the genus Hirschmanniella. The evolutionary history was inferred by using the maximum likelihood method and Kimura 2-parameter model. The tree with the highest log likelihood (−3,442.77) is shown. The percentage of trees in which the associated taxa clustered together is shown next to the branches. A discrete Gamma distribution was used to model evolutionary rate differences among sites (5 categories (+G, parameter  =  0.3519)). The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. This analysis involved 25 nucleotide sequences. There were a total of 795 positions in the final data set. Accession preceded by ♦ is a new sequence.

Figure 5:

Phylogenetic relationship based on ITS rRNA sequences within the genus Hirschmanniella. The evolutionary history was inferred by using the maximum likelihood method and Tamura 3-parameter model. The tree with the highest log likelihood (−4,485.75) is shown. The percentage of trees in which the associated taxa clustered together is shown next to the branches. A discrete Gamma distribution was used to model evolutionary rate differences among sites (5 categories (+G, parameter  =  0.9061)). The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. This analysis involved 16 nucleotide sequences. There were a total of 1,486 positions in the final data set. Accession preceded by ♦ is a new sequence.

Morphometrics of Hirschmanniella dicksoni n_ sp_

FemaleMale
CharacterHolotypeParatypesParatype
n 1920
L 1,9061714 ± 1521506 ± 143
(1,582–1,929)(1,235–1,772)
a 6159 ± 4.960 ± 6.3
(50–69)(47–72)
b 1111 ± 1.19.6 ± 0.9
(10–14)(8.6–11.3)
b′ 4.14.2 ± 0.44.3 ± 0.4
(3.8–4.8)(3.8–5.1)
c 1314 ± 0.915 ± 1.5
(13–15)(13–18)
c′ 6.76.2 ± 0.56.2 ± 0.8
(5.1–6.7)(4.8–7.2)
G12223 ± 2.3
(20–26)
G22020 ± 1.8
(17–24)
T37 ± 5
(29–45)
V5553 ± 1.7
(50–56)
M5152 ± 1.754 ± 1.9
(51–55)(51–57)
o2322 ± 2.221 ± 2.9
(19–27)(17–27)
DGO4.54.3 ± 0.44 ± 0.6
(3.8–5.1)(3.3–5.1)
Lip region diameter1010 ± 0.79.9 ± 0.4
(9.1–11.2)(9.2–11)
Lip region height3.74.1 ± 0.34.3 ± 0.3
(3.7–4.8)(4.0–5.1)
Stylet length1920 ± 0.619 ± 0.5
(19–21)(18–20)
Conus length9.810 ± 0.510 ± 0.4
(9.7–11)(9.6–11)
Shaft length9.59.3 ± 0.48.7 ± 0.4
(8.6–9.9)(8.2–9.6)
Stylet knob height32.7 ± 0.42.7 ± 0.2
(2–3.4)(2.4–3.2)
Stylet knob width4.64.5 ± 0.44.3 ± 0.5
(3.8–5.3)(3.4–5.0)
Procorpus length8063 ± 13.870 ± 7.2
(50–87)(54–80)
Median bulb length1717 ± 1.416 ± 1.3
(15–20)(14–18)
Median bulb diam.1312 ± 0.811 ± 1.4
(11–13)(7.5–13)
Median bulb valve length5.85 ± 0.74.7 ± 0.7
(3.5–6.3)(3.8–6)
Median bulb valve width3.62.9 ± 0.42.9 ± 0.5
(2.3–3.6)(2.1–3.6)
Anterior end to oesophageal-intestinal junction177154 ± 12158 ± 13
(142–177)(129–179)
Anterior end to excretory (EP) pore146133 ± 7119 ± 11
(124–146)(87–134)
EP pore to oesophageal-intestinal junction3125 ± 4.339 ± 8.9
(20–32)(24–50)
Metacorpus valve from anterior end8985 ± 4.180 ± 4.7
(80–94)(72–87)
Nerve ring from anterior end116113 ± 5102 ± 10
(105–122)(73–114)
Oesophageal gland overlap294257 ± 34193 ± 23
(204–311)(150–223)
Oesophagus length470411 ± 38351 ± 30
(330–470)(303–392)
Max body length3129 ± 1.626 ± 3.1
(26–31)(21–30)
Length from phasmids to terminus3229 ± 232 ± 2.9
(25–32)(27–35)
Tail length149125 ± 11102 ± 11
(103–149)(84–119)
Anal body width2220 ± 117 ± 1.8
(18–22)(15–20)
From cloaca to anterior most part of testis 559 ± 92
(420–708)
Vulva to anterior end1045912 ± 90
(803–1056)
Anterior genital branch420391 ± 57
(305–472)
Posterior genital branch385333 ± 39
(289–433)
Ant. Spermatheca length2628 ± 4.2
(20–35)
Ant. Spermatheca diam1516 ± 2.6
(9.3–19)
Post. Spermatheca length2424 ± 3.9
(17–30)
Post. Spermatheca diam1414 ± 2
(8.9–17)
Bursa70 ± 15
(45–94)
Cloaca to anterior tip of bursa25 ± 5.8
(15–33)
Cloaca to posterior tip of bursa45 ± 9.5
(27–61)
Gubernaculum8.9 ± 1.1
(7.4–11)
Spicules25 ± 2.9
(19–30)
DOI: https://doi.org/10.21307/jofnem-2019-083 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
Language: English
Page range: 1 - 15
Submitted on: Aug 3, 2017
|
Published on: Dec 4, 2019
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year

© 2019 Alemayehu W. Habteweld, Faruk Akyazi, Soumi Joseph, William T. Crow, Eyualem Abebe, Tesfamariam Mekete, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.