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Developing a real-time PCR diagnostic method for a potential threat to chrysanthemum, Paratylenchus dianthus

Journal of Nematology
Volume 51 (2019): Issue 1 (January 2019)
By:
Open Access
|Aug 2019

Figures & Tables

Figure 1:

Results of pot experiments. A: Plant height; B: Plant dry weight of above-ground parts; and C: SPAD value (only for Ch05 and Ch12); and D: Pin-nematode densities with soil treated with imicyafos (3 kg a.i./ha), or non-treated as a control in triplicates, after 2-month growth period. Asterisks indicate significant difference from a control group by Student’s t-test (*p < 0.05 and **p < 0.01). Different alphabet letters indicate significant difference by a multiple comparison (Tukey–Kramer’s test, main effect (treatment): p < 0.05; interaction effect (treatment × soil: p > 0.05) following two-way ANOVA (alphabet letters in Figure 1D are only for reference; interaction effect (treatment × soil): p < 0.05).
Results of pot experiments. A: Plant height; B: Plant dry weight of above-ground parts; and C: SPAD value (only for Ch05 and Ch12); and D: Pin-nematode densities with soil treated with imicyafos (3 kg a.i./ha), or non-treated as a control in triplicates, after 2-month growth period. Asterisks indicate significant difference from a control group by Student’s t-test (*p < 0.05 and **p < 0.01). Different alphabet letters indicate significant difference by a multiple comparison (Tukey–Kramer’s test, main effect (treatment): p < 0.05; interaction effect (treatment × soil: p > 0.05) following two-way ANOVA (alphabet letters in Figure 1D are only for reference; interaction effect (treatment × soil): p < 0.05).

Figure 2:

Relationship between calibration curves of soil DNA samples and nematode lysate for Paratylenchus dianthus. Solid line: a calibration curve derived from DNA samples extracted from Ch12 experiment at two-month after planting. Dotted line: a calibration curve derived from DNA samples extracted from nematode lysate. The curve was related to the DNA concentrations equivalent to the ones obtained by using DNA samples from soils. ***p < 0.001 and **p < 0.01.
Relationship between calibration curves of soil DNA samples and nematode lysate for Paratylenchus dianthus. Solid line: a calibration curve derived from DNA samples extracted from Ch12 experiment at two-month after planting. Dotted line: a calibration curve derived from DNA samples extracted from nematode lysate. The curve was related to the DNA concentrations equivalent to the ones obtained by using DNA samples from soils. ***p < 0.001 and **p < 0.01.
DOI: https://doi.org/10.21307/jofnem-2019-043 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
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Language: English
Page range: 1 - 11
Submitted on: Feb 12, 2019
Published on: Aug 5, 2019
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year
Keywords:
chrysanthemum,
diagnosis,
high-throughput,
host–parasitic relationship,
imicyafos,
pathogen,
pin-nematode,
plant-parasitic nematode,
pot experiment,
quantitative PCR,
real-time PCR,
technique
Related subjects:
Life sciences,
Life sciences, other

© 2019 Masanori Kawanobe, Koki Toyota, Hidehito Uchihara, Mikoto Takae, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.

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