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Morphological and molecular characterization of Labronema montanum sp. n. (Dorylaimida, Dorylaimidae) from Spain

Journal of Nematology
Volume 51 (2019): Issue 1 (January 2019)
By:
Open Access
|Jun 2019

Figures & Tables

Figure 1:

Labronema montanum sp. n. from Spain. (A, B): Anterior region in lateral, median view. (C): Pharyngeal expansion. (D): Female, entire. (E): Male, entire. (F): Female, anterior genital branch. (G): Lip region in lateral, surface view. (H): Pharyngo-intestinal junction. (I): Vagina. (J): Spicule. (K, L): Female tail. (M): Male tail. (N): Lateral guiding piece. (Scale bars: A, B, G, I–M = 10 µm; C, F = 50 µm; D, E = 500 µm; H = 20 µm; N = 5 µm).
Labronema montanum sp. n. from Spain. (A, B): Anterior region in lateral, median view. (C): Pharyngeal expansion. (D): Female, entire. (E): Male, entire. (F): Female, anterior genital branch. (G): Lip region in lateral, surface view. (H): Pharyngo-intestinal junction. (I): Vagina. (J): Spicule. (K, L): Female tail. (M): Male tail. (N): Lateral guiding piece. (Scale bars: A, B, G, I–M = 10 µm; C, F = 50 µm; D, E = 500 µm; H = 20 µm; N = 5 µm).

Figure 2:

Labronema montanum sp. n. from Spain (female, LM). (A): Entire. (B, C): Anterior region in lateral median view. (D): Lip region in lateral, surface view. (E, I): Vagina. (F): Anterior genital branch. (G): Pharyngeal expansion. (H): Pharyngeal enlargement. (J–M): Tail. (Scale bars: A = 500 µm; B–D, H–M = 10 µm; F, G = 50 µm.)
Labronema montanum sp. n. from Spain (female, LM). (A): Entire. (B, C): Anterior region in lateral median view. (D): Lip region in lateral, surface view. (E, I): Vagina. (F): Anterior genital branch. (G): Pharyngeal expansion. (H): Pharyngeal enlargement. (J–M): Tail. (Scale bars: A = 500 µm; B–D, H–M = 10 µm; F, G = 50 µm.)

Figure 3:

Labronema montanum sp. n. from Spain (male, LM). (A): Entire. (B–D): Pharyngo-intestinal junction showing the three lobes. (E): Posterior body region. (F): Spicule. (G, H): Male. (I): Lateral guiding piece. (J): Sperm cells. (Scale bars: A = 500 µm; D–B = 20 µm; E = 50 µm; F–H = 10 µm; I = 5 µm; J = 10 µm).
Labronema montanum sp. n. from Spain (male, LM). (A): Entire. (B–D): Pharyngo-intestinal junction showing the three lobes. (E): Posterior body region. (F): Spicule. (G, H): Male. (I): Lateral guiding piece. (J): Sperm cells. (Scale bars: A = 500 µm; D–B = 20 µm; E = 50 µm; F–H = 10 µm; I = 5 µm; J = 10 µm).

Figure 4:

Labronema montanum sp. n. from Spain (SEM). (A): Lip region in ventral view. (B): Same in frontal view. (C): Vulva, ventral view. (D, E): Male, posterior body region showing ad-cloacal pair (D) and ventromedian series (E) of genital papillae. (F): Female tail, ventral view. (G, H): Female tail, lateral view, showing indentations of body cuticle.
Labronema montanum sp. n. from Spain (SEM). (A): Lip region in ventral view. (B): Same in frontal view. (C): Vulva, ventral view. (D, E): Male, posterior body region showing ad-cloacal pair (D) and ventromedian series (E) of genital papillae. (F): Female tail, ventral view. (G, H): Female tail, lateral view, showing indentations of body cuticle.

Figure 5:

The Bayesian tree inferred from known and the newly sequenced Labronema montanum sp. n. based on sequences of the 28S rRNA region.
The Bayesian tree inferred from known and the newly sequenced Labronema montanum sp. n. based on sequences of the 28S rRNA region.

Morphometrics of Labronema montanum sp_ n_ from Spain_ Measurements in μm except L in mm, and in the form: average ± sd (range)_

HolotypeParatypesParatypes
Character♀17♀♀11♂♂
L1.741.83 ± 0.14 (1.56–2.07)1.87 ± 0.12 (1.68–2.08)
a17.821.3 ± 2.0 (17.8–24.4)22.7 ± 1.8 (20.3–25.9)
b3.73.8 ± 0.3 (3.3–4.3)3.7 ± 0.2 (3.3–4.1)
c6268.1 ± 9.4 (56–86)72.2 ± 5.5 (65–84)
V5858.4 ± 1.2 (56.5–60.2)–
c’0.60.6 ± 0.1 (0.5–0.8)0.6 ± 0.0 (0.6–0.7)
Lip region diameter2322.0 ± 1.1 (19–23)22.2 ± 1.3 (19–24)
Odontostyle length ventral side2725.0 ± 1.9 (21–28)26.0 ± 2.0 (22–29)
Odontostyle length dorsal side2826.5 ± 2.1 (23–30)27.3 ± 2.0 (23–30)
Odontophore length4342.0 ± 1.9 (37–44)42.7 ± 2.2(38–45)
Neck length474476 ± 27 (417–514)500 ± 31 (450–551)
Pharyngeal expansion length238242 ± 19 (205–272)247 ± 16 (212–264)
Body diam. at neck base9280.6 ± 8.3 (65–98)78.9 ± 6.1 (72–91)
 mid-body9885.9 ± 7.6 (72–99)82.8 ± 8.9 (71–101)
 anus/cloaca4443.9 ± 3.3 (39–51)42.3 ± 3.7 (36–47)
Distance vulva – anterior end10151068 ± 88 (889–1232)–
Prerectum length93125 ± 23 (84–160)202 ± 34 (150–268)
Rectum/cloaca length6160.0 ± 4.1 (52–68)71.6 ± 5.6 (61–80)
Tail length2827.4 ± 4.5 (19–35)26.1 ± 2.9 (20–30)
Spicules length––71.3 ± 3.6 (65–76)
Ventromedian supplements––(20–25)
DOI: https://doi.org/10.21307/jofnem-2019-029 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
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Language: English
Page range: 1 - 11
Submitted on: Mar 26, 2019
Published on: Jun 3, 2019
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year
Keywords:
Description,
Dorylaims,
LSU,
Morphology,
New species,
SEM,
Taxonomy
Related subjects:
Life sciences,
Life sciences, other

© 2019 R. Peña-Santiago, J. Abolafia, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.

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