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Nematicidal Activity of Fosthiazate Against Soybean Cyst Nematode Heterodera glycines

Open Access
|Apr 2019

Figures & Tables

Figure 1

Mortality rate (%) of H. glycines J2 exposure to fosthiazate with exposure time.
Mortality rate (%) of H. glycines J2 exposure to fosthiazate with exposure time.

Figure 2

The shape of H. glycines J2. (A, B) normal shape of J2; (B) natural shape of normal dead J2; (C, D) the shape of dead J2 killed by fosthiazate. Bar: A, C:100 µm, B, D:50 µm.
The shape of H. glycines J2. (A, B) normal shape of J2; (B) natural shape of normal dead J2; (C, D) the shape of dead J2 killed by fosthiazate. Bar: A, C:100 µm, B, D:50 µm.

Figure 3

Cumulative hatching percentage of H. glycines free eggs exposure to fosthiazate with exposure time. The error bars in the picture referred to standard error.
Cumulative hatching percentage of H. glycines free eggs exposure to fosthiazate with exposure time. The error bars in the picture referred to standard error.

Effect of fosthiazate on control of H_ glycines in the pot experiment_

TreatmentAmount of fosthiazate (a.i.) (mg)Average number of cystPopulation decrease (%)
Water0412 a
0.5% DMSO0432 a
2.18 mg/l0.0044389 ab5.43
5.45 mg/l0.0109232 bc43.64
13.62 mg/l0.0272194 c53.00
34.04 mg/l0.0681135 cd67.21
85.10 mg/l0.17029 d97.94

Mortality rate (%) of H_ glycines J2 at different durations after exposure to different concentrations of fosthiazate_

Exposure time (hr)
Concentration (mg/l)24681012
13.622.86 ± 0.74 a9.80 ± 1.15 a19.48 ± 2.29 a32.96 ± 3.81 a73.04 ± 2.52 a86.35 ± 1.33 a
8.611.97 ± 0.86 ab7.96 ± 0.85 a13.54 ± 0.48 b26.32 ± 2.04 b67.23 ± 3.14 b79.77 ± 2.72 b
5.451.55 ± 0.61 abc5.07 ± 0.90 b10.90 ± 0.86 bc22.64 ± 1.22 b39.15 ± 1.68 c66.82 ± 3.26 c
3.442.24 ± 0.70 ab5.17 ± 0.99 b8.10 ± 1.16 cd11.18 ± 1.62 c21.35 ± 1.88 d48.39 ± 1.93 d
2.180.89 ± 0.60 bc4.29 ± 1.20 bc6.41 ± 0.98 d6.41 ± 0.98 cd10.01 ± 0.65 e13.43 ± 0.57 e
0.5% DMSO0.52 ± 0.52 bc1.93 ± 1.00 cd1.90 ± 0.73 e2.27 ± 0.68 de2.27 ± 0.68 f3.23 ± 0.80 f
Water0.00 ± 0.00 c0.00 ± 0.00 d0.00 ± 0.00 e0.00 ± 0.00 e0.00 ± 0.00 f0.00 ± 0.00 f

Toxicity of fosthiazate to H_ glycines J2 at different treatment exposure time_

Exposure time (h)Slope(±SE)Correlation coefficientLC50 (95%CI)LC90 (95%CI)
40.54 (± 0.10)0.953912.59 (317.10−48275.85)941773.86 (9,541.28−92957,985.01)
81.38 (± 0.18)0.9825.00 (16.53−37.80)212.43 (83.90−537.87)
122.65 (± 0.44)0.974.41 (3.53−5.50)13.41 (9.36−19.20 )

Lengths of H_ glycines J2 at 60 h after replaced fosthiazate with distilled water

Length (µm)
TreatmentStyletEsophageal glands to headHyaline regionEntire body
Fosthiazate24.16 ± 0.41 b155.11 ± 3.74 b57.98 ± 1.13 a371.77 ± 10.97 b
Water25.77 ± 0.33 a199.48 ± 2.03 a27.99 ± 0.59 b501.04 ± 8.44 a
DOI: https://doi.org/10.21307/jofnem-2019-021 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
Language: English
Page range: 1 - 9
Submitted on: Oct 22, 2018
Published on: Apr 23, 2019
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year

© 2019 Hai Yan Wu, Man Luo, Lu Yuan Zhang, Xun Bo Zhou, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.