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Broad-based root-knot nematode resistance identified in cowpea gene-pool two Cover

Broad-based root-knot nematode resistance identified in cowpea gene-pool two

Journal of Nematology
Volume 50 (2018): Issue 4 (January 2018)
By: Arsenio D. Ndeve,  William C. Matthews,  Jansen R. P. Santos,  Bao Lam Huynh and  Philip A. Roberts  
Open Access
|Dec 2018

Figures & Tables

Figure 1

Root-galling response of 24 test plus control genotypes under field infestation (2012) by avirulent (Avr Mi) and virulent Meloidogyne incognita (Mi) isolates “Beltran” and “Muller,” respectively, and aggressive M. javanica (Mj) isolate “Project 811”. Control genotypes CB27, NIL-2 and NIL-3 genes, CB50 and CB46 carry gene Rk, CB3-gg carries a root-galling resistance gene effective against Avr Mi and Mi, and CB46-Null is susceptible. Horizontal line is GI = 3 representing the cut-off between resistant and susceptible response. Bars indicate the standard error. The data are ranked by response to Mj, Mi, and Avr Mi.
Root-galling response of 24 test plus control genotypes under field infestation (2012) by avirulent (Avr Mi) and virulent Meloidogyne incognita (Mi) isolates “Beltran” and “Muller,” respectively, and aggressive M. javanica (Mj) isolate “Project 811”. Control genotypes CB27, NIL-2 and NIL-3 genes, CB50 and CB46 carry gene Rk, CB3-gg carries a root-galling resistance gene effective against Avr Mi and Mi, and CB46-Null is susceptible. Horizontal line is GI = 3 representing the cut-off between resistant and susceptible response. Bars indicate the standard error. The data are ranked by response to Mj, Mi, and Avr Mi.

Figure 2

Root-galling response of 48 genotypes following infection by avirulent (Avr Mi) and virulent Meloidogyne incognita (Mi) isolates “Beltran” and “Muller,” respectively, and by aggressive M. javanica (Mj) isolate “Project 811” under field infestation (2014). Control genotypes CB27, NIL-2 and NIL-3 genes, CB50 and CB46 carry gene Rk, CB3-gg carries a root-galling resistance gene effective against Avr Mi and Mi, and CB46-Null is susceptible. Horizontal line is GI = 3 representing the cut-off between resistant and susceptible response. Bars indicate the standard error. The data are ranked by response to Mj, Mi, and Avr Mi.
Root-galling response of 48 genotypes following infection by avirulent (Avr Mi) and virulent Meloidogyne incognita (Mi) isolates “Beltran” and “Muller,” respectively, and by aggressive M. javanica (Mj) isolate “Project 811” under field infestation (2014). Control genotypes CB27, NIL-2 and NIL-3 genes, CB50 and CB46 carry gene Rk, CB3-gg carries a root-galling resistance gene effective against Avr Mi and Mi, and CB46-Null is susceptible. Horizontal line is GI = 3 representing the cut-off between resistant and susceptible response. Bars indicate the standard error. The data are ranked by response to Mj, Mi, and Avr Mi.

Figure 3

Root-galling response of 48 genotypes to aggressive Meloidogyne javanica isolate “Project 811” in greenhouse inoculations. Control genotypes CB27, NIL-2 and NIL-3 genes, CB50 and CB46 carry gene Rk, CB3-gg carries a root-galling resistance gene effective against Avr Mi and Mi, and CB46-Null is susceptible. Horizontal line is GI = 3 representing the cut-off between resistant and susceptible response. Bars indicate the standard error.
Root-galling response of 48 genotypes to aggressive Meloidogyne javanica isolate “Project 811” in greenhouse inoculations. Control genotypes CB27, NIL-2 and NIL-3 genes, CB50 and CB46 carry gene Rk, CB3-gg carries a root-galling resistance gene effective against Avr Mi and Mi, and CB46-Null is susceptible. Horizontal line is GI = 3 representing the cut-off between resistant and susceptible response. Bars indicate the standard error.

Figure 4

Egg-mass production by avirulent Meloidogyne incognita (Avr Mi) isolate “Project 77” and by aggressive M. javanica “Project 811” on root systems of 48 cowpea genotypes screened using seedling growth-pouches in growth chamber. Control genotypes CB27, NIL-2 and NIL-3 genes, CB50 and CB46 carry gene Rk, CB3-gg carries a root-galling resistance gene effective against Avr Mi and Mi, and CB46-Null is susceptible. Horizontal line is EM = 30 representing the cut-off between resistant and susceptible response. Bars indicate the standard error. The data are ranked by response to Mj and Avr Mi.
Egg-mass production by avirulent Meloidogyne incognita (Avr Mi) isolate “Project 77” and by aggressive M. javanica “Project 811” on root systems of 48 cowpea genotypes screened using seedling growth-pouches in growth chamber. Control genotypes CB27, NIL-2 and NIL-3 genes, CB50 and CB46 carry gene Rk, CB3-gg carries a root-galling resistance gene effective against Avr Mi and Mi, and CB46-Null is susceptible. Horizontal line is EM = 30 representing the cut-off between resistant and susceptible response. Bars indicate the standard error. The data are ranked by response to Mj and Avr Mi.

Figure 5

Relationship between root-galling and egg-mass production under Meloidogyne javanica “Project 811” infestation. Correlation established using average root-galling and egg-mass data from two greenhouse and three growth chamber experiments, respectively.
Relationship between root-galling and egg-mass production under Meloidogyne javanica “Project 811” infestation. Correlation established using average root-galling and egg-mass data from two greenhouse and three growth chamber experiments, respectively.

Figure 6

Relationship between root-galling induced by (A): virulent Meloidogyne incognita “Muller” and M. javanica “Project 811” and (B): avirulent M. incognita “Beltran” and M. javanica “Project 811”, based on average root-galling data from 2014 field experiment.
Relationship between root-galling induced by (A): virulent Meloidogyne incognita “Muller” and M. javanica “Project 811” and (B): avirulent M. incognita “Beltran” and M. javanica “Project 811”, based on average root-galling data from 2014 field experiment.

Figure 7

(A): Average virulence index of Meloidogyne javanica “Project 811”, avirulent (Avr) and virulent M. incognita isolates “Beltran” and “Muller,” respectively, based on average root-galling data from 2014 field experiment; (B): Average virulence index of M. javanica “Project 811” and Avr M. incognita “Project 77,” based on average egg-mass production in growth chamber experiment.
(A): Average virulence index of Meloidogyne javanica “Project 811”, avirulent (Avr) and virulent M. incognita isolates “Beltran” and “Muller,” respectively, based on average root-galling data from 2014 field experiment; (B): Average virulence index of M. javanica “Project 811” and Avr M. incognita “Project 77,” based on average egg-mass production in growth chamber experiment.

Resistance gene sets in control genotypes and their response status to avirulent (Avr) and virulent Meloidogyne incognita and M_ javanica_

Root-knot nematode response
Genotype R gene setAvr. M. incognita M. incognita M. javanica
UCR779NoneSusceptibleSusceptibleSusceptible
CB46-NullNoneSusceptibleSusceptibleSusceptible
CB46 RkRk ResistantSusceptibleSusceptible
CB27 RkRk/rk3rk3 ResistantResistantResistant
CB50 RkRk ResistantM. ResistantM. Resistant
NIL-2 genes RkRk/QRk-vu9.1 ResistantM. ResistantResistant
NIL-3 genes RkRk/QRk-vu9.1/ a ResistantResistantResistant
CB3-gg Root-galling gene a ResistantResistantSusceptible
DOI: https://doi.org/10.21307/jofnem-2018-046 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
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Language: English
Page range: 545 - 558
Published on: Dec 3, 2018
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year
Keywords:
Broad-based resistance,
Cowpea,
Cowpea gene-pool two,
spp., Root-knot nematode
Related subjects:
Life sciences,
Life sciences, other

© 2018 Arsenio D. Ndeve, William C. Matthews, Jansen R. P. Santos, Bao Lam Huynh, Philip A. Roberts, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.

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