Table 1
Morphometrics of Aphelenchoides giblindavisi n. sp. from Iran. All measurements are in μm and in the form: mean ± S.D. (range).
| Female | Male | ||
|---|---|---|---|
| Character | Holotype | Paratypes | Paratypes |
| n | – | 12 | 12 |
| L | 653 | 671 ± 72.4 (546–795) | 632 ± 41.4 (523–679) |
| a | 39.6 | 34.9 ± 2.2 (31.4–39.6) | 37.4 ± 2.2 (32.4–41.0) |
| b | 8.6 | 8.9 ± 0.8 (7.4–10.1) | 8.7 ± 0.4 (8.0–9.4) |
| b′ | 4.5 | 4.5 ± 0.4 (3.9–5.0) | 4.5 ± 0.2 (4.2–5.0) |
| c | 18.7 | 18.9 ± 1.0 (17.2–20.4) | 18.9 ± 1.7 (15.8–21.5) |
| c′ | 3.5 | 3.4 ± 0.2 (3.1–3.7) | 2.8 ± 0.2 (2.6–3.1) |
| V or T | 70.1 | 70.6 ± 1.6 (68.8–73.5) | 52.8 ± 3.4 (47.4–59.2) |
| Head height | 2.5 | 2.5 ± 0.0 (2.0–2.5) | 2.5 ± 0.0 (2.0–2.5) |
| Head diam. | 5.5 | 5.5 ± 0.4 (5–6) | 5.5 ± 0.3 (5–6) |
| Stylet | 10.5 | 10.5 ± 0.5 (10–11) | 10.0 ± 0.4 (10–11) |
| Stylet conus | 4.5 | 4.5 ± 0.2 (4.0–4.5) | 4.0 ± 0.2 (3.5–4.5) |
| m | 42.9 | 38.9 ± 2.3 (36.4–42.9) | 38.4 ± 2.1 (35–40) |
| Median bulb | 58 | 58.0 ± 3.2 (54–65) | 56.0 ± 1.4 (54–58) |
| Excretory pore | 69 | 69.0 ± 6.2 (60–84) | 66.5 ± 1.8 (64–69) |
| Hemizonid | 80 | 89.0 ± 10.6 (77–110) | 81.0 ± 2.6 (78–85) |
| Pharynx | 76 | 75.5 ± 2.8 (71–81) | 72.0 ± 3.4 (65–78) |
| Nerve ring | 75 | 75.0 ± 2.8 (71–81) | 73.0 ± 2.3 (70–78) |
| Median bulb length | 13.5 | 14 ± 1 (12–15) | 13.0 ± 0.9 (11–14) |
| Median bulb diam. | 10.5 | 11.0 ± 1.1 (9.0–12.5) | 10.0 ± 0.9 (8.0–11.5) |
| Median bulb length/diam. | 1.3 | 1.3 ± 0.1 (1.1–1.4) | 1.3 ± 0.1 (1.2–1.6) |
| Pharyngeal overlapping | 70 | 73.0 ± 6.7 (63–87) | 67.0 ± 6.4 (60–80) |
| Maximum body diam. | 16.5 | 19.0 ± 2.4 (15–23) | 17.0 ± 1.4 (14–19) |
| Vulval body diam. (VBD) | 16 | 18.0 ± 1.8 (14.5–20.0) | – |
| Body diam. at median bulb | 13.5 | 14.0 ± 1.4 (12.0–15.5) | 13.0 ± 0.8 (11–14) |
| Postvulval uterine sac (PUS) | 65 | 62.4 ± 6.0 (52–69) | – |
| PUS/VBD | 4.1 | 3.5 ± 0.4 (2.7–4.1) | – |
| Vulva–anus | 160 | 161 ± 21.6 (114–190) | – |
| Ovary or testis length | 295 | 257 ± 58.7 (183–356) | 334 ± 35 (248–394) |
| Anal (cloacal) body width | 10 | 10 ± 1 (9.0–12.5) | 12.0 ± 0.6 (10.5–13.0) |
| Tail length | 35 | 35.0 ± 2.5 (30–39) | 34.0 ± 1.8 (31–37) |
| Spicules length (arc line) | – | – | 17.0 ± 0.7 (16–18) |
| Capitulum | – | – | 7.0 ± 0.5 (6–8) |
Fig. 1
Line drawings of Aphelenchoides giblindavisi n. sp. (A, B) Entire body of female and male. (C) Anterior end. (D, E) Pharynx. (F) Part of female reproductive system. (G) Tail end. (H, I) Tail of female. (J) Posterior body region of male.
Fig. 2
Light microphotographs of Aphelenchoides giblindavisi n. sp. (A–C) Anterior region. (D) Part of female reproductive system (junction of oviduct and spermatheca). (E) Spicule. (F) Vulva region and postvulval uterine sac. (G, H) Posterior body region of female. (I) Tail end of male (All scale bars = 10 μm).
Fig. 3
Scanning electron microphotographs of Aphelenchoides giblindavisi n. sp. (A) Details of anterior region. (B, C) Lip region. (D, E) Lateral lines in different parts of mid-body. (F) Details of tail end. (G) Female posterior body region. (H, I) Male caudal region.
Fig. 4
Scanning electron microphotograph of Aphelenchoides giblindavisi n. sp. Details of lip region. Abbreviations: il9–inner lip papillae, aa–amphidial openings, olp–outer labial papillae, cp–cephalic papillae, ld–labial disc.
Table 2
Morphometrics of Iranian population of Robustodorus helicus n. comb. All measurements are in μm and in the form: mean ± S.D. (range).
| Females | |
|---|---|
| n | 15 |
| L | 464 ± 37.3 (395–547) |
| a | 29.2 ± 1.8 (26.7–33.5) |
| b | 7.8 ± 0.6 (6.7–8.8) |
| b′ | 4.6 ± 0.4 (3.8–5.4) |
| c | 19.7 ± 1.7 (16.8–22.8) |
| c′ | 2.8 ± 0.3 (2.4–3.1) |
| V | 69.3 ± 1.6 (64.4–71.1) |
| Head height | 2.0 ± 0.3 (2.0–2.5) |
| Head diam. | 5.0 ± 0.4 (4.5–5.5) |
| Stylet | 10.0 ± 0.4 (10–11) |
| Stylet conus | 4.0 ± 0.3 (4.0–4.5) |
| m | 41.3 ± 2.8 (36.4–45.0) |
| Median bulb | 47.5 ± 2.9 (39–51) |
| Excretory pore | 53.0 ± 4.8 (46–62) |
| Hemizonid | 63.0 ± 5.6 (53–69) |
| Pharynx | 60 ± 4 (49–65) |
| Nerve ring | 58 ± 4 (45–62) |
| Median bulb length | 11.5 ± 0.9 (10.0–13.5) |
| Median bulb diam. | 10.0 ± 0.8 (8–11) |
| Median bulb length/diam. | 1.2 ± 0.1 (1.1–1.3) |
| Pharyngeal overlapping | 40 ± 5 (31–49) |
| Maximum body diam. | 16.0 ± 1.6 (13–20) |
| Vulval body diam. (VBD) | 15.0 ± 1.4 (12–19) |
| Body diam. at median bulb | 13.0 ±1.1 (11.5–15.0) |
| Postvulval uterine sac (PUS) | 38 ± 8 (28–55) |
| PUS/VBD | 2.5 ± 0.5 (2.0–3.7) |
| Vulva–anus | 119 ± 13 (101–147) |
| Ovary length | 161 ± 20 (130–193) |
| Anal body diam. | 8.0 ± 0.9 (7–10) |
| Tail length | 24.0 ± 1.4 (21–26) |
Fig. 5
Line drawings of Iranian population of Robustodorus helicus n. comb. (A) Anterior end. (B, C) Female total body. (D, F) Pharynx. (E) Stylet. (G, H) Posterior body region.
Fig. 6
Light microphotographs of fresh females of Iranian population of Robustodorus helicus n. comb. (A) Anterior region. (B, H) Part of pharynx. (C) Anterior end. (D) Excretory pore. (E) Metacorpus. (F) Tail. (G) Embryo inside the mature egg inside the body of female. (All scale bars = 10 μm).
Fig. 7
Light microphotographs of Iranian population of Robustodorus helicus n. comb. (A) Three tulip-shaped lips in lateral view. (B, C) Three lines in the lateral field forming two bands. (D, E) Postvulval uterine sac. (F) Anterior region. (All scale bars = 10 μm).
Fig. 8
Bayesian tree inferred under the GTR + G + I model using SSU rDNA sequences of Aphelenchoides giblindavisi n. sp. and Robustodorus helicus n. comb. Posterior probability and bootstrap values exceeding 50% are given on appropriate clades in the form Bayesian posterior probability/maximum likelihood bootstrap value (BPP/BS). The new sequences are in bold font.
Fig. 9
Bayesian tree inferred under the GTR + G + I model using partial LSU rDNA sequence of Aphelenchoides giblindavisi n. sp. and Robustodorus helicus n. comb. Posterior probability and bootstrap values exceeding 50% are given on appropriate clades in the form Bayesian posterior probability/maximum likelihood bootstrap value (BPP/BS). The new sequences are in bold font. The asterisk on Laimaphelenchus hyrcanus Miraeiz et al. (2015) refers to the possible synonymy of the species with L. belgradiensis Oro, 2015 as discussed by Pedram et al. (2018a, 2018b).