Figure 1
Line drawings of female Delatylus andersoni n. gen., n. sp: (A) head and pharynx, (B) lip region and stylet, (C) deirids, (D) vulva and the anus viewed ventrally, (E) vulva and the tail viewed laterally, (F) oviduct and quadricolumella, (G) oocytes.
Figure 2
Micrographs of females of Delatylus andersoni n. gen., n. sp: (A) entire body; (B) head region, (C) pharynx (arrow showing the position of excretory pore and hemizonid), (D) pharynx with anterior part of the gonad, (E) posterior part of the female body, (F, G) female tail (arrow showing the position of vulva and anus), H: lateral lines. (scale bars = 10 μm).
Figure 3
Micrographs of valve at the pharyngeal–intestinal junction of females of Delatylus andersoni n. gen., n. sp.
Figure 4
SEM of Delatylus andersoni n. gen., n. sp. (A, B) En face view of head and lip (aa arrows point to the amphid aperture; oa arrows point to the oral aperture; op arrows point to outer labial papillae; (C) vulva, anus, and tail; (D) lateral fields.
Table 1
Morphometrics of Delatylus andersoni n. gen., n. sp. All measurements are in μm and in the form: mean ± s.d (range).
| Measurements | ||||
|---|---|---|---|---|
| Holotype | Populations | |||
| Characters | 9095 (paratype) | 480 | 484 | |
| n | 1 | 19 | 20 | 20 |
| L | 760 | 756.1 ± 113.7 (526.8–943.9) | 613.8 ± 96.3 (439.5–829.6) | 760.0 ± 73.8 (636.6–907.2) |
| a | 27.1 | 28.8 ± 2.4 (24.9–33.5) | 23.7 ± 2.7 (18.3–27.9) | 22.0 ± 2.7 (17.8–27.1) |
| b | 10.8 | 11.1 ± 1.7 (7.5–13.7) | 9.6 ± 1.6 (7.0–13.8) | 11.6 ± 1.4 (9.3–13.8) |
| b' | 5.6 | 5.9 ± 0.8 (4.1–7.1) | 5.8 ± 0.9 (4.3–8.3) | 6.6 ± 0.6 (5.4–7.4) |
| c | 13.8 | 14.3 ± 2.1 (10.3–17.9) | 13.7 ± 1.2 (11.3–15.5) | 15.0 ± 1.7 (11.8–18.9) |
| c' | 3.2 | 3.4 ± 0.2 (2.9–3.8) | 3.2 ± 0.2 (2.8–3.8) | 3.1 ± 0.4 (2.6–4.1) |
| V | 89 | 89.9 ± 1.5 (85.6–91.8) | 88.8 ± 0.8 (87.3–90.3) | 89.5 ± 1.1 (87.3–91.8) |
| Stylet length | 9.0 | 8.8 ± 0.3 (8.5–10.5) | 8.8 ± 0.6 (7.8–9.7) | 9.2 ± 0.7 (7.3–10.1) |
| Max. body diam. | 28 | 26.4 ± 4.6 (18.4–33.9) | 26.4 ± 6.2 (20.1–40.5) | 35.1 ± 6.3 (23.5–49.8) |
| Gonad length | 595 | 526.0 ± 10 (317.7–677.9) | 461.1 ± 91.5 (261.6–685.5) | 603.1 ± 74.7 (494.5–753.8) |
| Lip height | 2 | 2.1 ± 0.2 (1.8–2.6) | 2.2 ± 0.2 (1.9–2.8) | 2.3 ± 0.3 (2.0–2.9) |
| Lip width | 8 | 8.3 ± 0.5 (7.1–9.1) | 7.4 ± 0.6 (6.6–8.7) | 8.0 ± 0.7 (6.4–8.9) |
| Anterior end to nerve ring | 65 | 61.1 ± 3.8 (55.1–70.8) | 55.9 ± 3.2 (50.8–64.7) | 58.6 ± 4.5 (50.2–66.2) |
| Anterior end to pharyngo-intestinal junction | 70 | 68.3 ± 3.3 (62.5–75.1) | 63.9 ± 3.8 (58.1–72.3) | 65.7 ± 4.5 (55.8–73.0) |
| Anterior end to the hemizonid | 110 | 104.0 ± 8.2 (91.3–122.5) | 95.7 ± 13.1 (79.8–128.6) | 107.0 ± 7.5 (90.7–119.9) |
| Distance from the nerve ring to the hemizonid | 50 | 43.0 ± 6.4 (31.6–57.2) | 36.8 ± 13.0 (23.4–69.0) | 48.4 ± 9.2 (29.1–61.3) |
| Anterior end to the excretory pore | 113 | 107.1 ± 7.6 (95.6–124.5) | 92.6 ± 13.0 (76.7–125.4) | 103.0 ± 7.4 (86.0–114.6) |
| Distance from the nerve ring to the excretory pore | 51 | 46.0 ± 5.6 (37.8–59.2) | 36.8 ± 13.0 (23.4–69.0) | 44.4 ± 9.2 (24.4–58.1) |
| Anterior end to the end of pharyngeal glands | 135 | 128.3 ± 7.7 (113.2–146.6) | 106.4 ± 10.2 (90.5–127.4) | 115.0 ± 5.3 (105.7–125.3) |
| Distance from vulva to anus | 26 | 22.3 ± 3.3 (16.0–28.3) | 23.3 ± 3.6 (18.8–31.3) | 27.8 ± 3.0 (21.5–33.3) |
| Anal body width (ABW) | 17 | 15.6 ± 2.0 (11.3–19.8) | 14.1 ± 1.6 (11.9–16.9) | 16.5 ± 2.0 (12.5–21.0) |
| Distance from vulva to tail terminus | 81 | 75.3 ± 5.4 (60.0–83.9) | 67.9 ± 7.2 (55.2–84.9) | 78.8 ± 5.2 (72.8–89.3) |
| Tail length | 55 | 53.1 ± 4.6 (39.3–58.9) | 44.7 ± 4.8 (35.5–53.6) | 51.0 ± 4.0 (45.8–59.6) |
| Ratio of vulva–anus/tail length | 0.47 | 0.5 ± 0.1 (0.4–0.6) | 0.5 ± 0.2 (0.4–0.7) | 0.5 ± 0.1 (0.4–0.5) |
Figure 5
Phylogeny of Delatylus andersoni n. gen., n. sp. and closely related inferred from partial 18 S rRNA gene sequences by Bayesian analysis. Phylogeny was inferred under a GTR+I+G model (−lnL = 6333.834; AIC = 12687.668; freqA = 0.2384; freqC = 0.2036; freqG = 0.267; freqT = 0.2911; R(a) = 1.1233; R(b) = 3.698; R(c) = 2.4739; R(d) = 0.7402; R(e) = 5.1375; R(f) = 1; Pinva = 0.6096; Shape = 0.8015). Posterior probability values exceeding 50% are given on appropriate clades.
Figure 6
Phylogeny of Delatylus andersoni n. gen., n. sp. and closely related inferred from 28 S D2/D3 rRNA gene sequences by Bayesian analysis. Phylogeny was inferred under a TrN+G model (−lnL = 2644.97; AIC = 5301.9399; freqA = 0.2103; freqC = 0.1993; freqG = 0.3249; freqT = 0.2655; R(a) = 1; R(b) = 3.0953; R(c) = 1; R(d) = 1; R(e) = 7.2585; R(f) = 1; Pinva = 0; Shape = 0.3542). Posterior probability values exceeding 50% are given on appropriate clades.