Identification of HG Types of Soybean Cyst Nematode Heterodera glycines and Resistance Screening on Soybean Genotypes in Northeast China

Genotype category	Soybean germplasms
Glycine soja	ZYD00006;
Breeding lines	Sien1-2, Sien1-3, 09-138;
Peking-resistant cultivar to Heterodera glycines	Kangxian2, Kangxian4, Kangxian5, Kangxian6, Kangxian7, Kangxian8, Kangxian9, Kangxian10, Kangxian11, Kangxian12, Kangxian13;
Local commercial cultivars	Dongsheng1, Suinong14, Hefeng25

Table 2

Heterodera glycines female indices^a and HG Types associated with three different nematode populations.

Location	1(PI 548402)	2(PI 88788)	3(PI 90763)	4(PI 437654)	5( PI 209332)	6 (PI 89772)	7(PI 548316)	Pickett	HG Type²
Anda1	71.93	24.03	25.21	0.00	33.45	21.57	66.72	78.38	1.2.3.5.6.7
Anda2	45.83	1.75	13.20	0.19	5.24	0.24	9.90	160.30	1.3
Wuchang	0.00	44.56	0.00	0.00	22.28	1.10	42.63	95.80	2.5.7

[i] ^aFemale index (FI) = (average number of females on indicator lines)/(average number of females on Lee 68) × 100 (n=10).

[ii] ^bThe average number of cysts on susceptible check ‘Lee 68’ was equal to or more than 100.

Table 3

Response of germplasms to soybean cyst nematodes against HG Type 1.3.

		Test 1			Test 2
Genotype	Cyst/plant	FI^a	R^b	Cyst/plant	FI	R
Lee	92 ± 25.63abcde			211 ± 21.76defg
ZYD00006	56 ± 11.59abcde	60.43	S	131 ± 6.39bc	61.90	S
09-138	0 ± 0.2a	0.22	R	15 ± 2.52a	7.01	R
Sein1-2	115 ± 15.73cde	125.00	S	251 ± 22.65fg	119.05	S
Sein1-3	92 ± 23.33abcde	99.57	S	277 ± 29.01g	131.18	S
Kangxian2	66 ± 11.65abcde	71.52	S	127 ± 8.45bc	60.09	S
Kangxian4	121 ± 20.89de	131.52	S	197 ± 8.17cdefg	93.36	S
Kangxian5	124 ± 31.32de	134.78	S	251 ± 21.24fg	118.96	S
Kangxian6	125 ± 23.05de	135.87	S	253 ± 15.28fg	120.09	S
Kangxian7	44 ± 10.76abcd	47.39	MS	174 ± 19.66cdef	82.65	S
Kangxian8	47 ± 15.78abcd	50.87	MS	172 ± 6.92cdef	81.71	S
Kangxian9	100 ± 25.53bcde	108.70	S	219 ± 14.02defg	103.60	S
Kangxian10	98 ± 23.97bcde	106.96	S	147 ± 16.31cd	69.76	S
Kangxian11	140 ± 16.95e	152.17	S	167 ± 7.80cde	79.34	S
Kangxian12	22 ± 3.67ab	23.48	MR	64 ± 7.52ab	30.24	MS
Kangxian13	27 ± 6.24abc	29.13	MR	66 ± 2.21ab	31.28	MS
Hefeng25	118 ± 12.4cde	128.48	S	241 ± 27.16efg	114.22	S
Dongsheng1	101bcde	110.00	S	152 ± 25.63cd	71.85	S
Suinong14	80abcde	86.52	S	180 ± 13.62cdef	85.12	S

[i] Means (n = 5) followed by the same letter are not significantly different (P < 0.05) according to Tukey’s HSD test. The comparison was conducted within same column.

[ii] ^a FI: Female Index (FI) = (average number of females on cultivars)/(average number of females on Lee 68) × 100.

[iii] ^b R: Rating based on female index, where FI < 10 is resistant (R), FI = 10-30 is moderately resistant (MR), FI = 31-60 is moderately susceptible (MS), and FI > 60 is susceptible (S).

Table 4

Response of germplasms to soybean cyst nematodes against HG Type 2.5.7.

		Test 1			Test 2
Genotype	Cyst/plant	FI^a	R^b	Cyst/plant	FI	R
Lee	199±35.09bc			166±20.92b
ZYD00006	112±15.03ab	56.08	MS	132±2.91b	79.76	S
09-138	188±24.56bc	94.27	S	127±20.05b	76.63	S
Sein1-2	266±30.92c	133.57	S	142±13.89b	85.66	S
Sein1-3	290±53.11c	145.73	S	179±17.35b	108.07	S
Kangxian2	17±3.4a	8.64	R	26±23.46a	15.90	MR
Kangxian4	2±0.73a	1.11	R	1±0.58a	0.48	R
Kangxian5	8±1.74a	3.92	R	5±1.84a	3.01	R
Kangxian6	41±7.48a	20.60	MR	5±1.72a	3.25	R
Kangxian7	19±7.14a	9.75	R	14±6.62a	8.19	R
Kangxian8	68±12.7a	33.97	MS	16±3.35a	9.76	R
Kangxian9	6±0.97a	2.91	R	0±0a	0.00	R
Kangxian10	8±2.69a	4.22	R	2±1.17a	0.96	R
Kangxian11	12±4.42a	6.13	R	1±1.20a	0.72	R
Kangxian12	0±0.2a	0.10	R	0±0.20a	0.12	R
Kangxian13	3±0.86a	1.61	R	0±0a	0.00	R
Hefeng25	264±45.27c	132.46	S	137±22.92b	82.41	S
Dongsheng1	254±17.42c	127.64	S	118±14.55b	70.84	S
Suinong14	239±30.59c	120.00	S	147±20.95b	88.55	S

[i] Means (n = 5) followed by the same letter are not significantly different (P < 0.05) according to Tukey’s HSD test. The comparison was conducted within same column.

[ii] ^aFI: Female Index (FI) = (average number of females on cultivars)/ (average number of females on Lee 68) × 100.

[iii] ^bR: Rating ^based on female index, where FI < 10 is resistant (R), FI = 10-30 is moderately resistant (MR), FI = 31-60 is moderately susceptible (MS), and FI > 60 is susceptible (S).

Table 5

Response of germplasms to soybean cyst nematodes against HG Type 1.2.3.5.6.7.

		Test 1			Test 2
Genotype	Cyst/plant	FI^a	R^b	Cyst/plant	FI	R
Lee	56±4.39bcde			57±16.47abcde
ZYD00006	64±14.27cdef	113.93	S	78±6.25bcde	137.19	S
09-138	4±1.45a	7.14	R	22±14.45ab	38.95	MS
Sein1-2	103±12.48ef	183.21	S	86±25.77bcde	150.53	S
Sein1-3	71±14.37cdef	126.07	S	70±8.77abcde	122.11	S
Kangxian2	106±11.5f	188.93	S	33±8.58abc	58.60	MS
Kangxian4	86±6.5cdef	154.29	S	73±8.62abcde	128.42	S
Kangxian5	45±12.46abc	80.00	S	30±10.89abc	51.93	MS
Kangxian6	61±7.04cdef	108.57	S	53±6.39abcd	93.33	S
Kangxian7	55±11.51bcd	98.21	S	38±3.44abc	65.96	S
Kangxian8	63±13.66cdef	111.79	S	75±19.11abcde	131.58	S
Kangxian9	41±6.83abc	72.86	S	19±6.04ab	33.33	MS
Kangxian10	56±6.69bcde	99.29	S	68±1.63abcde	118.60	S
Kangxian11	78±4.5cdef	138.93	S	43±13.66abc	75.09	S
Kangxian12	13±1.56ab	23.57	MR	41±16.71abc	71.49	S
Kangxian13	13±2.12ab	23.21	MR	9±3.51a	15.44	MR
Hefeng25	101±10.23def	180.71	S	114±9.96de	200.70	S
Dongsheng1	64±5.42cdef	113.93	S	122±22.11e	213.33	S
Suinong14	83±9.05cdef	148.57	S	97±15.21cde	169.12	S

[i] Means (n = 5) followed by the same letter are not significantly different (P < 0.05) according to Tukey’s HSD test. The comparison was conducted within same column.

[ii] ^a FI: Female Index (FI) = (average number of females on cultivars)/(average number of females on Lee 68) × 100.

[iii] ^b R: Rating based on female index, where FI < 10 is resistant (R), FI = 10-30 is moderately resistant (MR), FI = 31-60 is moderately susceptible (MS), and FI > 60 is susceptible (S).

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DOI: https://doi.org/10.21307/jofnem-2018-007 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X

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Language: English

Page range: 41 - 50

Accepted on: Jun 27, 2017

Published on: May 31, 2018

Published by: Society of Nematologists, Inc.

In partnership with: Paradigm Publishing Services

Publication frequency: 1 issue per year

Keywords:

Glycine max ,

Heterodera glycines ,

HG type,

resistance screening,

soybean,

Soybean cyst nematodes

Related subjects:

Life sciences,

Life sciences, other

© 2018 Cui Hua, Chunjie Li, Yanfeng Hu, Yanzhi Mao, Jia You, Mingze Wang, Jingsheng Chen, Zhongyan Tian, Congli Wang, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.

Volume 50 (2018): Issue 1 (January 2018)