Potential influence of compounds released in degradation of phytates on the course of alcoholic fermentation of high gravity mashes – simulation with analogs of these compounds

Dawid Mikulski; Aleksandra J. Rolbiecka; Grzegorz R. Kłosowski

doi:10.1515/pjct-2017-0044

Abstract

Aim of the study was to evaluate the effect of supplementation of high gravity media with mineral compounds and myo-inositol, at concentration which would be obtained as a result of degradation of phytates present in raw material during alcoholic fermentation. The process of alcoholic fermentation was conducted under laboratory conditions in a 72 h system at 37°C with the use of S. cerevisiae D-2 strain. Calcium chloride proved to be the most effective of all supplements tested. Final ethanol concentration increased by 1.2% v v⁻¹ and the yield of process increased by ca. 7 dm⁻³ ethanol 100 kg⁻¹ of starch in comparison with control. Selective supplementation with KH₂PO₄, ZnSO₄ and MgSO₄ also increased the ethanol concentration, but the effect was accompanied by a deterioration in composition of volatile products. The hydrolysis of phytate complexes with microbial phytases can be an alternative solution to supplementation of HG mashes presented in this work.

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Potential influence of compounds released in degradation of phytates on the course of alcoholic fermentation of high gravity mashes – simulation with analogs of these compounds

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