Chemical Composition, Antioxidant and Antibacterial Properties of Chloroform Fraction of Platycerium Bifurcatum

Ikpa Chinyere Benardette Chinaka; Onoja Samuel Okwudili; Dozie-Akaniro Ijeoma Nkiru

doi:10.1515/arls-2018-0019

Abstract

The study investigated the bioactive constituents, antioxidant and antibacterial activities of Platycerium bifucartum leaves. Chloroform fraction of P. bifurcatum was prepared by partitioning the ethanol extract with chloroform and water. The phytochemical analysis was carried out using standard methods. Fourier transformer-infrared (FT-IR) and Gas chromatography-mass spectroscopy (GC/MS) were used in the characterization of the bioactive compounds. The antioxidant and antibacterial activities of the chloroform fraction were evaluated using standard protocols. The fourier transformer-infrared analysis showed the presence of C=O, OH, CHO, C-F and -NH functional groups. GC/MS characterization gave benzeneethaneamine (33.3%), 2-amino-1-(4-methylphenyl) propane (17.04%), hydroxyurea (30.26%) and epinephrine (13.26). The extract inhibited the growth of the bacterial isolates. The fraction exhibited antioxidant properties that were comparable with ascorbic acid. The presence of these compounds showed that the leaves of Platycerium bifurcatum can be used for the treatment of some bacterial diseases.

References

1. Agbo, M.O., Nnadi, C.O., Ukwueze, N.N., & Okoye, F.B.C. (2014). Phenolic constituents from Platycerium bifurcatum and their antioxidatant properties. Journal of Natural Products, 7, 48-57. http://www.journalofnaturalproducts.com/Volume7/7_Res_paper-6.pdf
Search in Google Scholar Back to article
2. Holtum, J.A., & Winter, K. (1999). Degrees of crassulacean acid metabolism in tropical epiphytic and lithophytic ferns. Functional Plant Biology, 26 (8), 749-757. https://doi.org/10.1071/PP9900110.1071/PP99001
Search in Google Scholar Back to article
3. Ojo, O.O., Ajayi, A.O., & Anibijuwon, I.I. (2007). Antibacterial potency of methanol extracts of lower plants. Journal of Zheijang University Science B, 8 (3), 189-191. https://doi.org/10.1631/jzus.2007.B018910.1631/jzus.2007.B0189181038517323431
Open DOI Search in Google Scholar Back to article
4. Paliwal, S.K., Sati, B., Faujdar, S., & Sharma, S. (2017). Antioxidant and antibacterial activities of various extracts of Inula cuspidata CB Clarke stem. Beni-seuf University Journal of Applied Sciences, 6 (2), 97-105. https://doi.org/10.1016/j.bjbas.2016.10.00310.1016/j.bjbas.2016.10.003
Open DOI Search in Google Scholar Back to article
5. Stalons, D.R., & Thornsberry, C. (1975). Brothdilution method for determining the antibiotic susceptibility of anaerobic bacteria. Antimicrobial Agents Chemotherapy, 7 (1), 15-21. http://aac.asm.org/content/7/1/15.full.pdf10.1128/AAC.7.1.154290641137356
Search in Google Scholar Back to article
6. Ezeja, M.I., Omeh, Y.N., Onoja, S.O., & Ukaonu, I.H. (2015). Anti-inflammatory and antioxidant activities of the methanolic leaf extract of Cissus aralioides. American Journal of Pharmacological Sciences 3 (1), 1-6. DOI: 10.12691/ajps-3-1-1
Search in Google Scholar Back to article
7. Onoja, S.O., Ezeja, M.I., Omeh, Y.N., & Onwukwe, B.C. (2016). Antioxidant, anti-inflammatory and antinociceptive activities of methanolic extract of Justicia secunda Vahl leaf. Alexandria Journal of Medicine, 53(3), 207-213. https://doi.org/10.1016/j.ajme.2016.06.00110.1016/j.ajme.2016.06.001
Open DOI Search in Google Scholar Back to article
8. Kos, I., Jadrijević-Mladar, M., Butula, I., Biruš, M., Maravić-Vlahoviček, G., & Dabelić, S. (2013). Synthesis, antibacterial and cytotoxic activity evaluation of hydroxyurea derivatives. Acta Pharmaceutica, 63 (2), 175-191. DOI: 10.2478/acph-2013-0014 10.2478/acph-2013-001423846141
Search in Google Scholar Back to article
9. Krishnanunni, K., Senthilvel, P., Ramaiah, S. & Anbarasu, A. (2015). Study of chemical composition and volatile compounds along with in-vitro assay of antioxidant activity of two medicinal rice varieties: Karungkuravai and Mappilai samba. Journal of Food Science and Technology, 52(5), 2572-2584. doi: 10.1007/s13197-014-1292-z
Open DOI Search in Google Scholar Back to article
10. Altaee, N., Kadhim, M.J., & Hameed, I.H. (2016). Characterization of metabolites produced by E. Coli and analysis of its chemical compounds using GC-MS. International Journal of Current Pharmaceutical Review and Research. 7 (6), 393-399. www.ijcpr.com
Search in Google Scholar Back to article
11. Das, S., Vasudeva, N., & Sharma, S. (2014). Chemical composition of ethanol extract of Macrotyloma uniflorum (Lam.) Verdc. using GC-MS spectroscopy. Organic and Medicinal Chemistry Letters, 4 (1), 13. https://doi.org/10.1186/s13588-014-0013-y10.1186/s13588-014-0013-y497043326548989
Open DOI Search in Google Scholar Back to article
12. Harikrishnan, R., Kim, M.C., Kim, J.S., Balasundaram, C., Jawahar, S., & Heo, M.S. (2010). Identification and antimicrobial activity of combined extract from Azadirachta indica and Ocimum Sanctum. Israeli journal of aquaculture = Bamidgeh, 62 (2), 2010, 85-95. http://hdl.handle.net/10524/1931710.46989/001c.20579
Search in Google Scholar Back to article
13. Thanga, K.K.S., Muthukumarasamy, S. & Mohan, V.R. (2012). GC-MS determination of bioactive components of Canscora perfoliata Lam.(Gentianaceae). Journal of Applied Pharmaceutical Science, 2 (8), 210-214. DOI: 10.7324/JAPS.2012.283710.7324/JAPS.2012.2837
Open DOI Search in Google Scholar Back to article
14. Rang, H.P., Dale, M.M., Ritter, J.M., & Moore, R.K. (2003). Pharmacology 5th edition, Edinburgh, Churchill livingstone.
Search in Google Scholar Back to article
15. Kounis, N.G., Soufras, G.D., Lianas, D., & Patsouras, N. (2016). After administration of intravenous epinephrine for bee sting-induced anaphylaxis: Kounis syndrome or epinephrine effect? Chinese Medical Journal, 129, 500-501. doi: 10.4103/0366-6999.176081
Open DOI Search in Google Scholar Back to article
16. Bahmani, M., Shirzad, H., Majlesi, M., Shahinfard, N. & Rafieian-Kopaei, M. (2014). A review study on analgesic applications of Iranian medicinal plants. Asian Pacific Journal of Tropical Medicine, 7, S43-S53. https://doi.org/10.1016/S1995-7645(14)60202-910.1016/S1995-7645(14)60202-9
Open DOI Search in Google Scholar Back to article
17. Hoffman, E.A., McCulley, A., Haarer, B., Arnak, R., & Feng, W. (2015). Break-seq reveals hydroxyureainduced chromosome fragility as a result of unscheduled conflict between DNA replication and transcription. Genome Research, 25 (3), 402-412. doi/10.1101/gr.180497.114.10.1101/gr.180497.114435288225609572
Open DOI Search in Google Scholar Back to article
18. Julián, E., Baelo, A., Gavaldà, J., & Torrents, E. (2015). Methyl-hydroxylamine as an efficacious antibacterial agent that targets the ribonucleotide reductase enzyme. PLoS ONE 10(3), e0122049. doi:10.1371/journal.pone.0122049
Open DOI Search in Google Scholar Back to article
19. Platt, O.S. (2008). Hydroxyurea for the treatment of sickle cell anemia. New England Journal of Medicine, 358 (13), 1362-1369. DOI: 10.1056/NEJMct070827210.1056/NEJMct070827218367739
Open DOI Search in Google Scholar Back to article
20. Yuh-Hwa, L.I.U., Wen-Chun, W.U., Yeh-Lin, L.U., Ying-Jang, L.A.I., & Wen-Chi, H.O.U. (2010). Antioxidant and amine oxidase inhibitory activities of hydroxyurea. Bioscience Biotechnology Biochemistry, 74 (6), 1256-1260. DOI: 10.1271/bbb.1000910.1271/bbb.10009
Open DOI Search in Google Scholar Back to article
21. Nurain, I.O., Bewaji, C.O., Johnson, J.S., Davenport, R.D., & Zhang, Y., (2016). Potential of three ethnomedicinal plants as antisickling agents. Molecular pharmaceutics. 14(1), 172-182. DOI: 10.1021/acs.molpharmaceut.6b0076710.1021/acs.molpharmaceut.6b00767596352528043127
Open DOI Search in Google Scholar Back to article
22. Pecinka, A., & Liu, C.H. (2014). Drugs for plant chromosome and chromatin research. Cytogenetic and Genome Research, 143 (1-3), 51-59. https://doi.org/10.1159/00036077410.1159/00036077424685908
Open DOI Search in Google Scholar Back to article
23. Petersen, M., Hofius, D., & Andersen, S.U. (2014). Signaling unmasked: autophagy and catalase promote programmed cell death. Autophagy. 10(3), 520-521. https://doi.org/10.4161/auto.27564 .10.4161/auto.27564407789024412967
Open DOI Search in Google Scholar Back to article

Chemical Composition, Antioxidant and Antibacterial Properties of Chloroform Fraction of Platycerium Bifurcatum

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