Prevalence and some virulence genes of Escherichia coli O157 isolated from chicken meats and giblets

Husnu Sahan Guran; Aydın Vural; Mehmet Emin Erkan; Halil Durmusoglu

doi:10.1515/aoas-2016-0061

Abstract

Escherichia coli O157 related foodborne illnesses continue to be one of the most important global public health problems in the world. This study aims to determine E. coli O157 prevalence in 375 chicken meat parts and giblets. The samples were collected randomly from several supermarkets and butchers in Diyarbakir, a city in southeast Turkey. They were analyzed and confirmed using the immunomagnetic separation (IMS), Vitek^® 2 microbial identification system and polymerase chain reaction (PCR) method. This study also aims to detect the presence of fliC_H7, eaeA, stx₁, stx₂ and hlyA genes by using PCR. The overall E. coli O157 prevalence in chicken meat parts and giblets was 1.3%. All of the E. coli O157 isolates carried rfbE_O157 and eaeA genes; but not any fliC_H7 and hlyA genes. The E. coli O157 isolates obtained from drumstick and breast meat carried either stx₁ or stx₂ genes, which were related to important virulence factors of the disease.

References

Abong’o B.O., Momba M.N.B. (2008). Prevalence and potential link between E. coli O157: H7 isolated from drinking water, meat and vegetables and stools of diarrheic confirmed and non-confirmed HIV/AIDSpatients in the Amathole District-South Africa. J. Appl. Microbiol., 105: 424-431.
Search in Google Scholar Back to article
Adams N.L., Byrne L., Smith G.A., Elson R., Harris J.P., Salmon R., Smith R., O’Brien S.J., Adak G.K., Jenkins C. (2016). Shiga toxin-producing Escherichia coli O157, England and Wales, 1983-2012. Emerg. Infect. Dis., 22, p. 590.
Search in Google Scholar Back to article
Ahmed A.M., Shimamoto T. (2014). Isolation and molecular characterization of Salmonella enterica, Escherichia coli O157: H7 and Shigella spp. from meat and dairy products in Egypt. Int. J. Food Microbiol., 168: 57-62.
Search in Google Scholar Back to article
Akbar A., Sitara U., Ali I., Iftikhar Khan M., Phadungchob T., Anal A.K. (2014). Presence of Escherichia coli in poultry meat: Apotential food safety threat. Int. Food Res. J., 21: 941-945.
Search in Google Scholar Back to article
Allerberger F. (2015). Poultry and human infections. Clin. Microbiol. Infect., 22: 101-102.
Search in Google Scholar Back to article
Alonso M.Z., Lucchesi P.M.A., Rodríguez E.M., Parma A.E., Padola N.L. (2012). Enteropathogenic (EPEC) and Shigatoxigenic Escherichia coli (STEC) in broiler chickens and derived products at different retail stores. Food Control, 23: 351-355.
Search in Google Scholar Back to article
Baker C.A., Rubinelli P.M., Park S.H., Carbonero F., Ricke S.C. (2016). Shiga toxinproducing Escherichia coli in food: Incidence, ecology, and detection strategies. Food Control, 59: 407-419.
Search in Google Scholar Back to article
Cagney C., Crowley H., Duffy G., Sheridan J.J., O’Brien S., Carney E., Anderson W., Mcdowell D.A., Blair I.S., Bishop R.H. (2004). Prevalence and numbers of Escherichia coli O157: H7 in minced beef and beef burgers from butcher shops and supermarkets in the Republic of Ireland. Food. Microbiol., 21: 203-212.
Search in Google Scholar Back to article
Chinen I., Epszteyn S., Melamed C.L., Aguerre L., Espinosa E.M., Motter M.M., Baschkier A., Manfredi E., Miliwebsky E., Rivas M. (2009). Shiga toxin-producing Escherichia coli O157 in beef and chicken burgers and chicken carcasses in Buenos Aires, Argentina. Int. J. Food. Microbiol., 132: 167-171.
Search in Google Scholar Back to article
Erdogan H., Levent B., Erdogan A., Gulesen R.,. Arslan H. (2011). Investigation of verotoxigenic Escherichia coli O157:H7 incidence in gastroenteritis patients. Mikrobiyol. Bul., 45: 519-525.
Search in Google Scholar Back to article
Eurosurveillance (1999). Sporadic cases of VTEC O157 infection associated with travel to southern Turkey, 3: 443-446. Available online: http://www.eurosurveillance.org/ViewArticle.aspx?ArticleId=1288
Search in Google Scholar Back to article
Fields P.I., Blom K., Hughes H.J., Helsel L.O., Feng P., Swaminathan B. (1997). Molecular characterization of the gene encoding Hantigen in Escherichia coli and development ofa PCR-restriction fragment length polymorphism test for identification of E. coli O157: H7 and O157: NM. J. Clin. Microbiol., 35: 1066-1070.
Search in Google Scholar Back to article
Fratamico P.M., Bagi L.K., Pepe T. (2000). Amultiplex polymerase chain reaction assay for rapid detection and identification of Escherichia coli O157: H7 in foods and bovine feces. J. Food. Prot., 63: 1032-1037.
Search in Google Scholar Back to article
Fukushima H., Seki R. (2004). High numbers of Shiga toxin-producing Escherichia coli found in bovine faeces collected at slaughter in Japan. FEMS Microbiol. Lett., 238: 189-197.
Search in Google Scholar Back to article
Gulesen R., Levent B., Demir T., Akgeyik M., Kuran S. (2016). Characterization of the Shiga toxin-producing Escherichia coli isolated from human between 2011 and 2014. Jpn. J. Infect. Dis., DOI: 10.7883/yoken.JJID.2015.346.10.7883/yoken.JJID.2015.34626902213
Search in Google Scholar Back to article
Hascelik G., Akan O.A., Diker S., Baykal M. (1991). Campylobacter and enterohaemorrhagic Escherichia coli (EHEC) associated gastroenteritis in Turkish children. J. Diarrhoeal Dis. Res., 9: 315-317.
Search in Google Scholar Back to article
Heiman K.E., Mody R.K., Johnson S.D., Griffin P.M., Gould L.H. (2015). Escherichia coli O157 outbreaks in the United States, 2003-2012. Emerg. Infect. Dis., 21, p. 1293.
Search in Google Scholar Back to article
Kalin R., Ongor H., Cetinkaya B. (2012). Isolation and molecular characterization of Escherichia coli O157 from broiler and human samples. Foodborne Pathog. Dis., 9: 313-318.
Search in Google Scholar Back to article
Karadal F., Ertas N., Hizlisoy H., Abay S., Al S. (2013). Prevalence of Escherichia coli O157: H7 and their verotoxins and Salmonella spp. in processed poultry products. J. Food Saf., 33: 313-318.
Search in Google Scholar Back to article
Karmali M.A., Gannon V., Sargeant J.M. (2010). Verocytotoxin-producing Escherichia coli (VTEC). Vet. Microbiol., 140: 360-370.
Search in Google Scholar Back to article
Kim S.R., Yoon Y., Seo M.K., Kim W.I., Shim W.B., Chung D., Yun J.C., Ryu K.Y., Kim B.S. (2014). Modification of methods for detection of Escherichia coli O157: H7 on produce. Food Sci. Biotechnol., 23: 1349-1356.
Search in Google Scholar Back to article
Manges A.R. (2015). Escherichia coli and urinary tract infections: the role of poultry-meat. Clin. Microbiol. Infect., 22: 122-129.
Search in Google Scholar Back to article
Maurer J.J., Schmidt D., Petrosko P., Sanchez S., Bolton L., Lee M.D. (1999). Development of primers to O-antigen biosynthesis genes for specific detection of Escherichia coli O157 by PCR. Appl. Environ. Microbiol., 65: 2954-2960.
Search in Google Scholar Back to article
Messens W., Bolton D., Frankel E., Liebana E., McLauchlin J., Morabito S., Oswald E., Threlfall E.J. (2015). Defining pathogenic verocytotoxin-producing Escherichia coli (VTEC) from cases of human infection in the European Union, 2007-2010. Epidemiol. Infect., 143: 1652-1661.
Search in Google Scholar Back to article
Mir R.A., Weppelmann T.A., Elzo M., Ahn S., Driver J.D., Jeong K.C. (2016). Colonization of beef cattle by Shiga toxin-producing Escherichia coli during the first year of life:acohort study. Plo Sone, 11: e0148518. doi:10.1371/journal.pone.0148518
Open DOI Search in Google Scholar Back to article
Momtaz H., Jamshidi A. (2013): Shiga toxin-producing Escherichia coli isolated from chicken meat in Iran: Serogroups, virulence factors and antimicrobial resistance properties. Poultry Sci., 92: 1305-1313.
Search in Google Scholar Back to article
Nataro J.P., Kaper J.B. (1998). Diarrheagenic Escherichia coli. Clin. Microbiol. Rev., 11: 142-201.
Search in Google Scholar Back to article
Ongor H., Kalin R., Cetinkaya B. (2007). Investigations of Escherichia coli O157 and some virulence genes in samples of meat and faeces from clinically healthy cattle in Turkey. Vet. Rec., 161: 392-394.
Search in Google Scholar Back to article
Paton A.W., Paton J.C. (1998). Detection and characterization of Shiga toxigenic Escherichia coli by using multiplex PCRassays for stx 1, stx 2, eae A, enterohemorrhagic E. coli hly A, rfb O111 and rfb O157. J. Clin. Microbiol., 36: 598-602.
Search in Google Scholar Back to article
Pilipcinec E., Tkáciková L., Naas H.T., Cabadaj R., Mikula I. (1999). Isolation of verotoxigenic Escherichia coli O157 from poultry. Folia Microbiol., 44: 455-456.
Search in Google Scholar Back to article
Sami M., Roya F. (2007). Prevalence of Escherichia coli O157: H7 on dairy farms in Shiraz, Iran by immunomagnetic separation and multiplex PCR. Iran J. Vet. Res., 4: 319-324.
Search in Google Scholar Back to article
Schmidt H., Beutin L., Karch H. (1995). Molecular analysis of the plasmid-encoded hemolysin of Escherichia coli O157: H7 strain EDL 933. Infect. Immun., 63: 1055-1061.
Search in Google Scholar Back to article
Schouten J.M., Van de Giessen A.W., Frankena K., De Jong M.C.M., Graat E.A.M. (2005). Escherichia coli O157 prevalence in Dutch poultry, pig finishing and veal herds and risk factors in Dutch veal herds. Prev. Vet. Med., 70: 1-15.
Search in Google Scholar Back to article
Smith - Palmer A., Locking M., Reilly B., Fisher I.S. (2005). Cluster of E. coli O157 infections in Scottish tourists returning from southwest Turkey, July-August (2005). Euro. Surveill., 10: E050818.2.
Search in Google Scholar Back to article
Tarr P.I., Gordon C.A., Chandler W.L. (2005). Shiga-toxin-producing Escherichia coli and haemolytic uraemic syndrome. The Lancet, 365: 1073-1086.
Search in Google Scholar Back to article
Wani S.A., Samanta I., Bhat M.A., Nishikawa Y. (2004). Investigation of shiga toxin-producing Escherichia coli in avian species in India. Lett. Appl. Microbiol., 39: 389-394.
Search in Google Scholar Back to article

Prevalence and some virulence genes of Escherichia coli O157 isolated from chicken meats and giblets

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