Expression of Annexin A1 and UCH-L1 in central nervous system neuroepithelial tumors

Takaew, Kanokkarn; Wongphoom, Jutamas; Ruangvejvorachai, Preecha; Shuangshoti, Shanop

doi:10.5372/1905-7415.0701.153

Abstract

Background: Pathological diagnosis of CNS neuroepithelial tumors can be problematic due to morphological similarity, and many tumors in the group share immunohistochemical profile. New markers are needed to enhance histologic diagnosis.

Objective: We studied Annexin A1 and UCH-L1 expression in CNS neuroepithelial tumors, with regard to a possible application for practical usage.

Methods: Expression of Annexin A1 and UCH-L1 was evaluated in 224 various neuroepithelial tumors by immunohistochemistry using tissue microarrays.

Results: Annexin A1 was expressed by the majority of ependymoma (92%) but none of central neurocytoma (p = 0.0000002) and medulloblastoma (p <10^-8). Absence of Annexin A1 and/or UCH-L1 was associated with low-grade astrocytoma rather than ependymoma (p = 0.0000009). Percentage of positive Annexin A1 and UCHL1 paralleled increasing histologic grades in diffuse astrocytomas.

Conclusion: Expression of Annexin A1 distinguishes ependymoma from central neurocytoma, and from medulloblastoma. Demonstration of Annexin A1 and/or UCH-L1 might be helpful to differentiate ependymoma from low-grade astrocytoma. Up-regulation of Annexin A1 and UCH-L1 in increasing histologic grade of diffuse astrocytomas suggests their roles in the malignant transformation.

References

1. Pan E, Prados MD. Primary neoplasm of the central nervous system. In: Kufe DW, Pollock RE, Weichselbaum RR, Bast RC, Gansler TS, Holland JF, Frei E, editors. Holland-Frei Cancer Medicine 6th ed. Ontario: BC Decker; 2003. p. 879-900.
Search in Google Scholar Back to article
2. Frosch MP, Antony DC, Girolami UD. The central nervous system. In: Kumar V, Abbas AK, Fauston N, editors. Robbins & Cotran Pathologic Basis of Disease 7th ed. Pennsylvania: Elsevier Inc; 2005. p. 1401-14.
Search in Google Scholar Back to article
3. Louis DN, Ohgaki H, Wiestler OD, Cavenee WK, Burger PC, Jouvet A, et al. The 2007 WHO classification of tumors of the central nervous system. Acta Neuropathol. 2007; 114:97-109.10.1007/s00401-007-0243-4
Open DOI Search in Google Scholar Back to article
4. Mckeever PE. Insights about brain tumors gained through immunohistochemistry and in situ hybridization of nuclear and phenotypic markers. J Histochem Cytochem. 1998; 46:585-94.10.1177/002215549804600504
Open DOI Search in Google Scholar Back to article
5. Chen D, Chen F, Yang X, Lu X, Zu Z, Pan J, et al. Identification of prohibitin as a potential biomarker for colorectal carcinoma base on proteomics technology. Int J oncol. 2010; 37:355-65.
Search in Google Scholar Back to article
6. Wang J, Dong Y, Wang X, Ma H, Sheng Z, Li G, et al. Expression of EphA1 in gastric carcinoma is associated with metastasis and survival. Oncol Rep. 2010; 24: 1577-84.
Search in Google Scholar Back to article
7. Ahn SH, Sawada H, RO JY, Nicolson GL. Differential expression of annexin I in human mammary ductal epithelial cells in normal and benign and malignant breast tissue. Clin Exp Metastasis. 1997; 15:151-6.10.1023/A:1018452810915
Open DOI Search in Google Scholar Back to article
8. Chen G, Gharib TG, Huang CC, Thomas DG, Shedden KA, Taylor JM, et al. Proteomics analysis of lung adenocarcinoma: identification of highly expressed set of protein in tumors. Clin Cancer Res. 2002; 7: 2298-305.
Search in Google Scholar Back to article
9. De Bont JM, den Boer ML, Kros JM, Pasier MM, Reddingius RE, Smitt PA, et al. Identification of novel biomarkers in pediatric primitive neuroectodermal tumor and ependymomas by proteome-wide analysis. J Neuropathol Exp Neurol. 2007; 66:505-16.10.1097/01.jnen.0000240475.35414.c3
Open DOI Search in Google Scholar Back to article
10. Park CK, Jung JH, Park SH, Jung HW, Cho BK. Multifarious proteomics signatures and regional heterogeneity in glioblastomas. J Neurooncol. 2009; 94:31-9.10.1007/s11060-009-9805-8
Open DOI Search in Google Scholar Back to article
11. Schittenhelm J, Trautmann K, Tabatabai G, Hermann C, Meyermann R, Beschorner R. Comparative analysis of annexin-1 in neuroepithelial tumors show altered expression with the grade of malignancy but is not associated with survival. Mod Pathol. 2009; 22: 1600-11.10.1038/modpathol.2009.132
Open DOI Search in Google Scholar Back to article
12. Johnso MD, Kamso-Pratt J, Pepsinsky RB, Whetsel WO Jr. Lipocortin in central and peripheral nervous system glial tumor. Hum Pathol. 1989; 20:772-6.10.1016/0046-8177(89)90071-3
Open DOI Search in Google Scholar Back to article
13. Zhang Y, Li Q, Zhu F, Cui J, Li K, Li Q, et al. Subcellular localization of APMCF I and its biological significance of expression pattern in normal and malignant human tissue. J Exp Clin Cancer Res. 2009; 28:111-6.10.1186/1756-9966-28-111
Open DOI Search in Google Scholar Back to article
14. Lim LH, Perraiz S. Annexin 1: The new face of an old molecule. FASEB J. 2007; 21:968-75.10.1096/fj.06-7464rev
Open DOI Search in Google Scholar Back to article
15. Allderidge LC, Bryant CE. Annexin 1 regulates cell proliferation by disruption of cell morphology and inhibition of cyclin D1 expression through sustained activation of ERK1/ 2 MAPK signal. Exp Cell Res. 2003; 290:93-107.10.1016/S0014-4827(03)00310-0
Search in Google Scholar Back to article
16. D’ Acquisto F, Merghani A, Lecona E, Rosignoli G, Raza K, Buckley CD, et al. Annexin-1 modulates T-cell activation and differentiation. Blood. 2007; 109: 1090-102.10.1182/blood-2006-05-022798
Search in Google Scholar Back to article
17. Solito E, de Coupade C, Canaider S, Goulding NJ, Perretti M. Transfection of Annexin 1 in monocytic cell produces a high degree of spontaneous and stimulate apoptosis associated with caspase-3 activation. Br J Pharmacol. 2001; 133:217-28.10.1038/sj.bjp.0704054
Open DOI Search in Google Scholar Back to article
18. Falini B, Tiacci E, Liso A, Basso K, Sabattini E, Pacini R, et al. Simple diagnostic assay for hairy cell leukemia by immunohistochemical detection of Annexin A1 (ANXA1). Lancet. 2004; 363:1869-70.10.1016/S0140-6736(04)16356-3
Open DOI Search in Google Scholar Back to article
19. Masaki T, Tokuda M, Ohnishi M, Watanabe S, Fujimura T, Miyamoto K, et al. Enhance expression of protein kinase substrate annexin in human hepatocellular carcinoma. Hepatology. 1996; 24:72-81.
Search in Google Scholar Back to article
20. Sinha P, Hutter G, Kottgen E, Dietel M, Schadendorf D, Larg H. Increased expression of annexin l and thioredoxin detect by two-dimensional gel electrophoresis of drug resistant human stomach cancer cells. J Biochem Biophys Methods. 1998; 37: 105-16.10.1016/S0165-022X(98)00020-7
Open DOI Search in Google Scholar Back to article
21. Bai XF, Ni XG, Zhao P, Lui SM, Wang HX, Gou B, et al. Over-expression of Annexin1 in pancreatic cancer and its clinical significance. World J Gastroenterol. 2004; 10:1466-70.10.3748/wjg.v10.i10.1466
Open DOI Search in Google Scholar Back to article
22. Xia SH, Hu LP, Hu H, Ying WT, Xu X, Cai Y, et al. Three isoform of Annexin l are preferentially expressed in normal esophageal epithelial but down regulate in esophageal squamous cell carcinomas. Oncogene. 2002; 21:6641-8.10.1038/sj.onc.1205818
Open DOI Search in Google Scholar Back to article
23. Kang JS, Calvo BF, Maygarden SJ, Caskey LS, Mohler JL, Ornstien DK. Dysregulation of annexin 1 protein expression in high-grade prostatic intraepithelial neoplasia and prostate cancer. Clin cancer Res. 2002; 8:117-23.
Search in Google Scholar Back to article
24. Garcia Pedrero JM, Fernandez MP, Morgan RO, Herrero ZA, Gonzailez MV, Suazez NC, et al. Annexin A1 down-regulation in head and neck cancer is associated with epithelial differentiation status. Am J Pahtol. 2004; 164:73-9.10.1016/S0002-9440(10)63098-2
Search in Google Scholar Back to article
25. Vishwanatha JK, Salazer E, Gopalakrishnan VK. Absence of annexin l expression in B-cell non- Hodgkin’s lymphomas and cell lines. BMC cancer. 2004; 4:8-16.10.1186/1471-2407-4-838523615070421
Open DOI Search in Google Scholar Back to article
26. Solito E, Mcarther S, Christian H, Gavin F, Buckingham JC, Gillines GE. Annexin A1 in the brain - undiscovered roles? Trends Phamacol Sci. 2008; 29:135-42.
Search in Google Scholar Back to article
27. Johnson MD, Gray ME, Pepinsky RB, Stahlman MT. Lipocortin-1 immunoreactivity in human pituitary gland. J Histochem Cytochem. 1990; 38:1814-45.10.1177/38.12.21477092147709
Open DOI Search in Google Scholar Back to article
28. Probst-Cousin S, Kowolik D, Kuchelmeister K, Kayser C, Neundorfer B, Heuss D. Expression of Annexin-1 in multiple sclerosis plaques. Neuropathol Appl Neurobiol. 2002; 28:292-300.10.1046/j.1365-2990.2002.00396.x12175341
Open DOI Search in Google Scholar Back to article
29. Liu Y, Fallow L, Lashuel HA, Lui Z, Lansbury PT Jr.
Search in Google Scholar Back to article
The UCH-L1 gene encodes two opposing enzimatic activites that affct alpha-Synuclein degradation and Parkinson’s disease susceptibility. Cell. 2002; 111: 209 -18.10.1016/S0092-8674(02)01012-7
Search in Google Scholar Back to article
30. Rolen U, Freda E, Xie J, Pfirrmann T, Frisan T, Masucci MG. The ubiquitin c-terminal hydrolase UCH-L1 regulates B-cell proliferation and integrin activation. J cell Mol Med. 2009; 13:1666-78.10.1111/j.1582-4934.2008.00501.x651238920187292
Open DOI Search in Google Scholar Back to article
31. Gavioli R, Frisan T, Vertuani S, Bornkamm GW, Masucci MG. c-myc overexpression activation alternative pathways for intracellular proteolysis in lymphoma cells. Nat Cell Biol. 2001; 3:283-8.10.1038/3506007611231578
Open DOI Search in Google Scholar Back to article
32. Tezel E, Hibi k, Nakasha T, Nakao A. PGP 9.5 as a prognostic factor in pancreatic cancer. Clin Cancer Res. 2006; 12:4764 -7.
Search in Google Scholar Back to article
33. Takase T, Hibi K, Yamazaki T, Nakayama H, Taguchi M, Kasia Y, et al. PGP9.5 Overexpression in esophageal squamous cell carcinoma. Hepatogastroenterology. 2003; 50:1278-80.
Search in Google Scholar Back to article
34. Okochi-Takada E, Nakazawa K, Wakabayashi M, Mori A, Ichimura S, Yasugi T, et al. Silencing of the UCHL1 gene in human colorectal and ovarian cancers. Int J cancer. 2006; 119:1338-44.10.1002/ijc.2202516642472
Search in Google Scholar Back to article
35. Takumaru Y, Yamashita K, Osada M, Nomoto S, Sun D, Xiao Y, et al. Inverse correlation between cyclin A1 Hypermethylation and P53 mutation in head and neck cancer identified by reversal of epigenetic silencing. Cancer Res. 2004; 64:5982-7.10.1158/0008-5472.CAN-04-099315342377
Search in Google Scholar Back to article
36. Yu J, Tao Q, Cheung KF, Jin H, Poon FF, Wang X, et al. Epigenetic identification of ubiquitin carboxylterminal hydrolase L1 as a functional tumor suppressor and biomarker for hepatocellular carcinoma and other digestive tumor. Hepatology. 2008; 48:508-18.10.1002/hep.2234318666234
Open DOI Search in Google Scholar Back to article
37. Day IN, Thomson RJ. UCHL1 (PGP 9.5): neuronal biomarker and ubiquitin system protein. Neurobiology. 2010; 90:327-62.
Search in Google Scholar Back to article
38. Sesuie R, Wada K. The functions of UCH-L1 and its relation to neurodegenerative diseases. Neurochem Int. 2007; 51:105-11.10.1016/j.neuint.2007.05.00717586089
Search in Google Scholar Back to article

Expression of Annexin A1 and UCH-L1 in central nervous system neuroepithelial tumors

Abstract

Paradigm

My account