Figure 1
Initial menu dialog presented to the user.
Figure 2
Example of a completed CATE template file. Cells containing ‘…’ are used to indicate that the spread-sheet contains data below what is presented in the figure. Note that completion of the ‘Vial #’ column under cell ‘A8’ is optional.
Figure 3
General architecture of vaCATE’s back-end. Modules are represented by white boxes, objects by orange boxes, object attributes by blue boxes, and methods/functions by green boxes. Forward slashes (‘/’) in attribute names are used to indicate the different forms that exist (e.g., obj_x/y_start means that both obj_x_start and obj_y_start attributes exist). Custom objects created by vaCATE can be assumed to belong to the Objects module. Note that this figure is meant to represent a general overview, and not necessarily the sequence in which data is processed.
Figure 4
Window displaying the current analysis applied to the data set. This allows users to dynamically change and preview analyses before export to an Excel (.xlsx) file.
Table 1
Names, descriptions and calculations of relevant parameters involved in compartmental analysis by tracer efflux (CATE). For more in-depth discussion regarding these parameters and their calculations, readers are directed to relevant works in the literature [1, 17, 20, 37, 38].
| Parameter name in | Description | Calculation | ||
|---|---|---|---|---|
| Literature | GUI*† | Backend† | ||
| So | SA | SA | Specific activity of loading solution | Radioactivity of loading solution (cpm/mL) |
| k | k | k | Rate constant | slope of regression line × 2.303 |
| to∙5 | Half-Life | t05 | Half-life of exchange | 0.693/k |
| Ro | – | r0 | Rate of radioisotope release at to | Antilog of x-intecept |
| φco | Efflux | efflux | Efflux from compartment | Ro/So |
| φnet | Net Flux | netflux | Net flux | (Root + shoot radioactivity)/So/root weight |
| φoc | Influx | influx | Influx | φco × φnet |
[i] * Graphical user interface; † of vaCATE.
Figure 5
The curvestrip() method in the Operations module is used to remove the effects of later, slower phases from earlier, faster phases.