Figure 1
(A) Representative actograms of Tppp -/- (top) and WT (bottom) during 12/12 LD photoperiod. Actogram data are single plotted (24h per line) with one line representing each day of the LD experiment. (B) Sin fit waves representing locomotor activity profiles of WT (solid line, n = 8) and Tppp -/- mice (dashed line, n = 8) with Circadian hours on the x-axis and signal amplitude on the y-axis. A solid vertical line (WT) and dashed vertical line (Tppp -/-) represent mean activity onset time of each group. (C & D) Representative actograms of WT (C) and Tppp -/- (D) and during DD photoperiod.
Table 1
Phenotypic characteristics of WT (n = 8) and Tppp -/- (n = 8) mice during 12/12 LD photoperiod. Values are displayed as mean ±SEM apart from rows representing FFT values, which are displayed as variance.
| LD CHARACTERISTIC | WT | TPPP -/- | T-TEST P VALUE |
|---|---|---|---|
| LD Period (hr.) | 23.98 ± .11 | 23.98 ± .12 | .34 |
| Mean LD activity/hour (counts) | 32.17 ± 3.6 | 26.89 ± 2.5 | <.001 |
| Mean acrophase (hr.) | 17.69 ± .12 | 16.73 ± .18 | .01 |
| Mean trough (hr.) | 5.7 ± .11 | 4.73 ± .15 | .03 |
| LD activity onset relative to lights off (hr.) | .01 ± .10 | –.89 ± .11 | .005 |
| FFT circadian amplitude (% total variance) | .38 ± .14 | .37 ± .16 | .55 |
| FFT ultradian amplitude (% total variance) | .08 ± .02 | .04 ± .02 | .09 |
Table 2
Phenotypic characteristics of WT (n = 4) and Tppp -/- (n = 4) mice during DD photoperiod. Values are displayed as mean ±SEM for DD period and mean activity, mean ±variance for FFT values.
| DD CHARACTERISTIC | WT | TPPP -/- | T-TEST P VALUE |
|---|---|---|---|
| DD Period (hr.) | 23.78 ± .08 | 24.63 ± .19 | .002 |
| Mean DD activity/hour (counts) | 30.21 ± 2.3 | 26.89 ± 1.5 | .001 |
| FFT circadian amplitude (% total variance) | .31 ± .1 | .25 ± .16 | .04 |
| FFT ultradian amplitude (% total variance) | .06 ± .01 | .01 ± .01 | .004 |
Figure 2
Serial clock gene expression in SCN; Tppp -/- v. WT at three time points. Values are displayed as Tppp -/- fold change relative to WT (the WT value for each transcript is represented by the dashed line at 1.00 on the y-axis). Asterisks indicate statistical significance at p < 0.008. Error bars represent SEM.
Figure 3
Serum melatonin concentration; WT (black) v. Tppp -/- (gray) at four timepoints (n = 3 per group per timepoint). Mean concentrations are displayed in ng/ml of serum. Asterisks indicate statistical significance for comparisons between groups (above each time point) and within the WT group over time (shown by the bracket).