Figure 1
Body weight differences between lean and obese mice. (A) Body weight gain after weaning shows the difference between lean and obese used. (B) Epididymal fat, liver, and kidney weights are shown as grams/Body Weight (g/BW) shows a substantial change in the size of fat tissue but not in other organs.
Figure 2
Circadian locomotor activity and photic phase response in lean and obese mice. In (A) representative actograms, star indicates the time when the light pulse (1 h) was given. Previous phase onset is projected with a solid gray line on the left side of each actogram. In (B) average phase shift at CT 6, 14, and 22 (n = 6, each) show differences in delays and advances between lean (white bars) and obese mice (black bars); (* p < 0.05, Student’s t test).
Figure 3
c-Fos immunostaining in cells of the half-medial SCN. (A) Representative samples. (B) Average (± SE) of the number of cells counted that express c-Fos. Mice without light pulse (Lean –P, Obese –P) and animals with light pulse (Lean +P, Obese +P) are shown. (* p < 0.05, Kruskal-Wallis test).
Figure 4
VIP immunostaining in the half-medial SCN. (A) Representative samples. (B) The average (± SE) of relative optic density evaluated from SCN slides in mice without light pulse (Lean –P, Obese –P) and with light pulse (Lean +P, Obese +P) are shown. (* p < 0.05, Kruskal-Wallis test).