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Developing the Tissue Engineered Heart Valve – a Descriptive Hemodynamic and Ultrasound in Vitro Characterization Study of Heart Valves in a Bioreactor Cover

Developing the Tissue Engineered Heart Valve – a Descriptive Hemodynamic and Ultrasound in Vitro Characterization Study of Heart Valves in a Bioreactor

Open Access
|May 2022

Figures & Tables

Figure 1

Tested valvular models – A – Decellularized porcine aortic valve; B – Decellularized porcine pulmonary valve; C- Biological prosthesis; D – Fresh porcine aortic valve; E – Fresh porcine pulmonary valve; F – mechanical valve.
Tested valvular models – A – Decellularized porcine aortic valve; B – Decellularized porcine pulmonary valve; C- Biological prosthesis; D – Fresh porcine aortic valve; E – Fresh porcine pulmonary valve; F – mechanical valve.

Figure 2

Ultrasound and photographic examination – A – general aspects of the heart valve bioreactor for pulmonary conditions and transversal plane technique, the ultrasound probe is marked in green; B – ultrasound aspect of longitudinal plane examination – the leaflets are marked with the red arrow; C- ultrasound view of transversal plane examination – the central coaptation of cusps is marked with the blue arrow. D – Snapshot of a decellularized porcine aortic valve; E – Snapshot of a biological prosthesis; F – Picture editing of the mechanical prosthesis used to calculate the opening area; G – Software evaluation of the opening are of a biological prosthesis.
Ultrasound and photographic examination – A – general aspects of the heart valve bioreactor for pulmonary conditions and transversal plane technique, the ultrasound probe is marked in green; B – ultrasound aspect of longitudinal plane examination – the leaflets are marked with the red arrow; C- ultrasound view of transversal plane examination – the central coaptation of cusps is marked with the blue arrow. D – Snapshot of a decellularized porcine aortic valve; E – Snapshot of a biological prosthesis; F – Picture editing of the mechanical prosthesis used to calculate the opening area; G – Software evaluation of the opening are of a biological prosthesis.

Ultrasound examination results in pulmonary and aortic conditions

Pulmonary conditions
TEHV ScaffoldFresh Pulmonary valveBiological valve prosthesisMechanical valve prosthesis
V max (cm/sec)328 ± 85355 ± 25260 ± 41181 ± 39
V mean (cm/sec)127 ± 28134 ± 10124 ± 19111 ± 7
P max (mmHg)55.12 (19.00–60.78)50.86 (45.98–57.63)26.89 (18.09–38.25)13.60 (7.36–20.40)
P mean (mmHg)11.82 ± 5.4212.26 ± 1.589.04 ± 2.474.42 ± 2.10
VTI (cm)45.54 ± 9.8347.46 ± 2.0942.23 ± 7.2330.71 ± 7.54
Functional area (cm2)1.14 (1.07–1.92)1.24 (1.17–1.29)1.43 (1.24–1.69)2.09 (1.68–3.03)
Aortic conditions
TEHV ScaffoldFresh Aortic valveBiological valve prosthesisMechanical valve prosthesis
V max (cm/sec)431 ± 64469 ±143368 ± 60399 ±121
V mean (cm/sec)130 ± 33163 ± 43132 ± 21161 ± 8
P max (mmHg)68.27 (60.28–100.75)75.37 (46.78–158.50)64.71 (33.00–68.08)85.60 (23.83–101.50)
P mean (mmHg)9.42 (8.08–22.00)14.69 (9.60–30.67)13.83 (7.00–14.51)17.15 (7.10–18.00)
VTI (cm)45.99 ± 11.3251.13 ± 15.8446.25 ± 7.6448.08 ± 12.94
Functional area (cm2)1.28 ± 0.271.14 ± 0.341.32 ± 0.261.26 ± 0.34

Video analysis in aortic and pulmonary conditions

TEHV scaffoldFresh valveBiological valve prosthesisMechanical valve prosthesis
Normalized pulmonary conditions peak opening area (cm2)2.916±0.1023.459±0.0992.018±0.0132.920±0.106
Normalized aortic conditions peak opening area (cm2)1.614±0.0463.330±0.1441.818±0.0872.756±0.015
DOI: https://doi.org/10.47803/rjc.2021.31.3.555 | Journal eISSN: 2734-6382 | Journal ISSN: 1220-658X
Language: English
Page range: 555 - 563
Published on: May 5, 2022
Published by: Romanian Society of Cardiology
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year

© 2022 Ionela Movileanu, Dan Octavian Nistor, Leslie Sierad, Klara Brinzaniuc, Codrut Oprita, Marius Mihai Harpa, Terezia Preda, Ovidiu Cotoi, Hussam AL Hussein, Hamida AL Hussein, Alexandru Chertes, Horatiu Suciu, Agneta Simionescu, Dan Simionescu, published by Romanian Society of Cardiology
This work is licensed under the Creative Commons Attribution 4.0 License.