Figure 1.
Figure 2.
Figure 3.
Figure 4.
List of primers used and their sequence
| Genes | Primer sequence |
|---|---|
| GAPDH-F | 5′-CTGACTTCAACAGCGACACC-3′ |
| GAPDH-R | 5′-TAGCCAAATTCGTTGTCATACC-3 |
| β Actin-F | 5′-CCAGAGCAAGAGAGGCATCC-3′ |
| β Actin-R | 5′-CCGTGGTGGTGAAGCTGTAG-3′ |
| SOX2-F | 5′-CCATCCACACTCACGCAAAA-3′ |
| SOX2-R | 5′-TATACAAGGTCCATTCCCCCG-3′ |
| OCT4-F | 5′-TCCCATGCATTCAAACTGAGG-3′ |
| OCT4-R | 5′-CCAAAAACCCTGGCACAAACT-3′ |
| NANOG-F | 5′-TGGACACTGGCTGAATCCTTC-3′ |
| NANOG-R | 5′-CGTTGATTAGGCTCCAACCAT-3′ |
| BMI 1-F | 5′-AATCCCCACCTGATGTGTGT-3′ |
| BMI 1-R | 5′-GCTGGTCTCCAGGTAACGAA-3′ |
| c-MYC-F | 5′-AAACACAAACTTGAACAGCTAC-3′ |
| c-MYC-R | 5′-ATTTGAGGCAGTTTACATTATGG-3′ |
| KLF 4-F | 5′-ATCTTTCTCCACGTTCGCGTCTG-3′ |
| KLF 4-R | 5′-AAGCACTGGGGGAAGTCGCTTC-3′ |
| EZH2-F | 5′-CCCTGACCTCTGTCTTACTTGTGGA-3′ |
| EZH2-R | 5′-ACGTCAGATGGTGCCAGCAATA-3′ |
| ALDH1A1-F | 5′-CGGGAAAAGCAATCTGAAGAGG-3′ |
| ALDH1A1-R | 5′-CCCGCTCAACACTCCTTCGA-3′ |
| S100A4-F | 5′-CCACAAGTACTCGGGCAAAG-3′ |
| S100A4-R | 5′-GTCCCTGTTGCTGTCCAAGT-3′ |
| TNNT1-F | 5′-GGTCAAGGCAGAACAGAAGC-3′ |
| TNNT1-R | 5′-GCGGTTGTACAGCACGTTGA-3′ |
Patient details
| Hospital No | Age | Biopsy findings | Endometrial cancer type |
|---|---|---|---|
| 639043B | 56 | Endometrioid adenocarcinoma Grade I-II | Type 1 endometrial cancer |
| 707263H | 60 | High grade endometrioid adenocarcinoma (poorly differentiated) Grade III | Type 2 endometrial cancer |