Primer sequences used in this study_
| GenE | Primer | Sequence (5’→3’) | Expected amplicon size (bp) |
|---|---|---|---|
| VIM | VIM-F | GATGGTGTTTGGTCGCATA | 390 |
| VIM-R | CGAATGCGCAGCACCAG | ||
| NDM | NDM-F | GCA GCT TGT CGG CCA TGC GGG C | 782 |
| NDM-R | GGT CGC GAA GCT GAG CAC CGC | ||
| KPC | KPC-F | TGT CAC TGT ATC GCC GTC | 900 |
| KPC-R | CTC AGT GCT CTA CAG AAA ACC | ||
| IMP | IMP-F | GGAATAGAGTGCTTAAYTCC | 232 |
| IMP-R | GGTTTAAYAACAAACACACC | ||
| OXA-48 | OXA-48-F | GCGTGGTTAAGGATGAACAC | 438 |
| OXA-48-R | CATCAAGTTCAACCCAACCG | ||
| mecC | mecC-F | GAAAAAAAGGCTTAGAACGCCTC | 138 |
| mecC-R | GAAGATCTTTTCCGTTTTCAGC | ||
| mecA | mecA-F | GTAGAAATGACTGAACGTCCGATAA | 310 |
| mecA-R | CCA ATT CCA CAT TGT TTC GGT CTA A |
Comparison of polymicrobial microorganisms detected by the BioFire® FilmArray BCID2 Panel and conventional blood culture results_
| Sample No. | BCID2 Results | Blood culture result | Clinical interpretation |
|---|---|---|---|
| 1, 2, 3, 14 | Staphylococcus epidermidis + Acinetobacter calcoaceticus/baumannii complex | Acinetobacter baumannii | Possible CoNS contamination |
| 4, 5 | Klebsiella pneumoniae group + Pseudomonas aeruginosa | Klebsiella pneumoniae or Pseudomonas aeruginosa | Predominant organism in culture |
| 6, 7, 10, 13, 15, 18 | Klebsiella pneumoniae group + Enterococcus faecalis + Staphylococcus spp. | Klebsiella pneumoniae | Possible polymicrobial BSI |
| 8, 12, 16 | Eschrichia coli + Klebsiella pneumoniae group + Enterococcus faecalis + Streptococcus spp. | No growth | Fastidious organisms / PCR sensitivity |
| 9 | Enterococcus faecalis + Proteus spp. | Enterococcus faecalis + Proteus mirabilis | Complete concordance |
| 11, 17 | Staphylococcus spp. + Pseudomonas aeruginosa | Pseudomonas aeruginosa | Possible CoNS contamination |
| 19 | Enterococcus faecalis + Staphylococcus spp. | Enterococcus faecalis | Predominant organism in culture |
| 20 | Eschrichia coli + Klebsiella pneumoniae group | Escherichia coli | Predominant organism in culture |
Comparison of BCID2 and blood culture results with concordance and diagnostic performance measures_
| Microorganism | BCID2-positive samples (n) | Growth in blood culture (n) | Concordance rate (%) | Sensitivity (%) | Specificity (%) | PPV (%) | NPV (%) |
|---|---|---|---|---|---|---|---|
| Acinetobacter calcoaceticus/baumannii complex | 14 | 9 | 64 | 100 | 87.8 | 64.3 | 100 |
| Klebsiella pneumoniae | 13 | 11 | 85 | 100 | 94.9 | 84.6 | 100 |
| Staphylococus epidermidis | 11 | 1 | 9 | 100 | 79.6 | 9.1 | 100 |
| Escherichia coli | 5 | 5 | 100 | 100 | 100 | 100 | 100 |
| Enterococcus faecalis | 5 | 1 | 20 | 100 | 91.8 | 20 | 100 |
| Pseudomonas aeruginosa | 4 | 4 | 100 | 100 | 100 | 100 | 100 |