
Fig. 1.
The pH value of liquid on the inner surface of the aligners at each sampling time. * means there is a statistical difference, p < 0.05.
Table I
The pH value at each time point.
| Time points | Number | pH value of aligner (mean ±S.D.) |
|---|---|---|
| Initial | 8 | 7.72 ± 0.319 |
| Group A-T0 | 8 | 7.72 ± 0.319 |
| Group A-T4 | 8 | 7.62 ± 0.171 |
| Group A-T8 | 8 | 7.53 ± 0.193 |
| Group A-T12 | 8 | 7.43 ± 0.261 |
| Group A-T24 | 8 | 7.42 ± 0.241 |
| Group B-T0 | 8 | 6.93 ± 0.260A |
| Group B-T4 | 8 | 7.43 ± 0.301 |
| Group B-T8 | 8 | 7.45 ± 0.329 |
| Group B-T12 | 8 | 7.23 ± 0.325A |
| Group B-T24 | 8 | 7.38 ± 0.162 |
| Group C-T0 | 8 | 6.83 ± 0.227A |
| Group C-T4 | 8 | 7.46 ± 0.107 |
| Group C-T8 | 8 | 7.47 ± 0.105 |
| Group C-T12 | 8 | 7.37 ± 0.191A, B |
| Group C-T24 | 8 | 7.38 ± 0.187 |

Fig. 2.
The alpha diversity and beta diversity of plaque microorganisms on the inner surface of aligners among groups. The order of the five lines in each set of data from the bottom to top indicates the minimum, first quartile, median, third quartile and maximum. * indicates a statistical difference, p < 0.05.

Fig. 3.
Bacterial composition distribution on the inner surface of the three groups at phylum level. * indicates a statistical difference, p < 0.05.

Fig. 4.
Comparison microbiome distribution on the inner surface of three groups at class and species level at 24h. * indicates a statistical difference, p < 0.05, ** indicates a statistical difference, p < 0.01.

Fig. 5.
Comparison of tooth surface after different rinsing methods.