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The Changes of Microbiome Attached on Clear Aligners after Drinking Coca-Cola Cover

The Changes of Microbiome Attached on Clear Aligners after Drinking Coca-Cola

By: DONG YAN,  Pengcheng Zuo,  Mei Lin and  Song Li  
Open Access
|Mar 2026

Figures & Tables

Fig. 1.

The pH value of liquid on the inner surface of the aligners at each sampling time. * means there is a statistical difference, p < 0.05.

Fig. 2.

The alpha diversity and beta diversity of plaque microorganisms on the inner surface of aligners among groups. The order of the five lines in each set of data from the bottom to top indicates the minimum, first quartile, median, third quartile and maximum. * indicates a statistical difference, p < 0.05.

Fig. 3.

Bacterial composition distribution on the inner surface of the three groups at phylum level. * indicates a statistical difference, p < 0.05.

Fig. 4.

Comparison microbiome distribution on the inner surface of three groups at class and species level at 24h. * indicates a statistical difference, p < 0.05, ** indicates a statistical difference, p < 0.01.

Fig. 5.

Comparison of tooth surface after different rinsing methods.

The pH value at each time point_

Time pointsNumberpH value of aligner (mean ±S.D.)
Initial87.72 ± 0.319
Group A-T087.72 ± 0.319
Group A-T487.62 ± 0.171
Group A-T887.53 ± 0.193
Group A-T1287.43 ± 0.261
Group A-T2487.42 ± 0.241
Group B-T086.93 ± 0.260A
Group B-T487.43 ± 0.301
Group B-T887.45 ± 0.329
Group B-T1287.23 ± 0.325A
Group B-T2487.38 ± 0.162
Group C-T086.83 ± 0.227A
Group C-T487.46 ± 0.107
Group C-T887.47 ± 0.105
Group C-T1287.37 ± 0.191AB
Group C-T2487.38 ± 0.187
DOI: https://doi.org/10.33073/pjm-2026-009 | Journal eISSN: 2544-4646 | Journal ISSN: 1733-1331
Language: English
Page range: 109 - 118
Submitted on: Sep 6, 2025
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Accepted on: Jan 25, 2026
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Published on: Mar 31, 2026
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year

© 2026 DONG YAN, Pengcheng Zuo, Mei Lin, Song Li, published by Polish Society of Microbiologists
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.