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Caries Direction Antimicrobial Activity of Zinc Oxide Nanoparticles Containing Cement for the Management of Orthodontic Teeth against Streptococcus mutans and Lactobacillus casei
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Caries Direction Antimicrobial Activity of Zinc Oxide Nanoparticles Containing Cement for the Management of Orthodontic Teeth against Streptococcus mutans and Lactobacillus casei

By: Aimin Wu and  Chungang Zhao  
Open Access
|Mar 2026

Figures & Tables

Fig. 1.

Initial setting time for the sample with and without ZnO particles.

Fig. 2.

Final setting time for the sample with and without ZnO particles.

Fig. 3.

Percentage of bacterial biomass for the test, i.e., cement with ZnO nanoparticles and control samples with no application of ZnO nanoparticles, with the use of Streptococcus mutans and Lactobacillus casei strains.

Percentage of biomass for the test groups and controls against Streptococcus mutans and also the combination of S_ mutans and Lactobacillus casei_

StrainStreptococcus mutantsStreptococcus mutants + Lactobacillus casei
Cements incorporated with ZnO nanoparticlesControl – No. ZnO nanoparticlesCements incorporated with ZnO nanoparticlesControl – No. ZnO nanoparticles
157.34100%40.5100%
243.22100%41.54100%
334.21100%37.45100%
442.11100%32.76100%
543.55100%35.376100%
643.11100%30.89100%
734.24100%36.84100%
832.77100%32.54100%
930.4100%29.32100%
1032.33100%25.38100%
Average39.2100%64.22100%
SD6.8905.050

Physicochemical properties of the OPC_

Chemical properties
MaterialSiO2 Al2O3 Fe2O3 CaOMgO
Cement20.925.123.951.146.11
MaterialSO3 Na2OK2OLoss on ignition
Cement2.930.220.923.21

Split tensile strength of cement containing different concentrations of ZnO nanoparticles_

Sample No.conc.ZnO TSplit tensile strength MPa
7 days14 days21 days28 days
Control03.94.24.24.3
S10.055.15.35.25.11
S20.155.415.255.195.04
S30.256.476.155.965.29
S40.56.876.286.176.04
S516.36.115.385.11

Flexural tensile strength with and without ZnO particles_

Sample No.conc.ZnO particlesFlexural tensile strength
7 days14 days21 days28 days
Control02.32.42.132.1
S10.052.52.42.342.31
S20.152.72.542.222.1
S30.252.882.442.12.1
S40.53.13.22.992.45
S5133.12.232.11
DOI: https://doi.org/10.33073/pjm-2026-003 | Journal eISSN: 2544-4646 | Journal ISSN: 1733-1331
Language: English
Page range: 11 - 19
Submitted on: May 11, 2025
|
Accepted on: Sep 7, 2025
|
Published on: Mar 31, 2026
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year

© 2026 Aimin Wu, Chungang Zhao, published by Polish Society of Microbiologists
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.