Fig. 1.
Fig. 2.
Fig. 3.
Identification of Candida species isolated from the surface of complete dentures using PCR with ITS1-ITS4 primers_
| Candida species | Color on HiCrome agar | Fragment size (bp) |
|---|---|---|
| Candida albicans | light green | 532 |
| Kluyveromyces marxianus | light pink | 722 |
| Candida tropicalis | blue | 521 |
| Nakaseomyces glabratus | cream to white | 874 |
| Meyerozyma. guilliermondii | mauve | 600 |
| Pichia kudriavzevii | purple, fuzzy | 500 |
| Candida dubliniensis | pale green | 540 |
| Pichia ethanolica | pink | ~ 450 |
| Candida. parapsilosis | cream to white | 516 |
Frequency of Candida species on the surface of complete dentures used by patients_
| Candida Species | Patient number | Percentage |
|---|---|---|
| Candida albicans | 23 | 46 |
| Kluyveromyces marxianus | 4 | 8 |
| Candida tropicalis | 4 | 8 |
| Nakaseomyces glabratus | 3 | 6 |
| Meyerozyma. guilliermondii | 2 | 4 |
| Pichia kudriavzevii | 1 | 2 |
| Candida dubliniensis | 1 | 2 |
| Pichia ethanolica | 1 | 2 |
| C. albicans and N. glabratus | 3 | 6 |
| C. albicans and K. marxianus | 1 | 2 |
| C. albicans and C. dubliniensis | 1 | 2 |
| C. albicans and C. tropicalis | 1 | 2 |
| C. albicans and P. ethanolica | 1 | 2 |
| C. albicans and C. parapsilosis | 1 | 2 |
| C. dubliniensis and C. tropicalis | 1 | 2 |
| C. tropicalis and N. glabratus | 1 | 2 |
| C. albicans, C. tropicalis, and M. guilliermondii | 1 | 2 |
| Total | 50 | 100 |
Demographic characteristics of study participants_
| Variable | Category | Number | Percentage |
|---|---|---|---|
| Gender | male | 35 | 35 |
| female | 65 | 65 | |
| Age group (years) | ≤40 | 5 | 5 |
| 41–59 | 41 | 41 | |
| ≥60 | 54 | 54 | |
| Denture wearing duration (months) | <6 | 27 | 27 |
| >6 | 73 | 73 | |
| Smoking status | yes | 7 | 7 |
| no | 93 | 93 | |
| Diabetic | yes | 31 | 31 |
| no | 69 | 69 |