Knocking Down LncRNA-GAS5 Restores the Inhibition of Cuprotosis Protein FDX-1 by Acinetobacter baumannii

An, Zhiyuan; Ding, Wenyi

doi:10.33073/pjm-2024-040

Abstract

This study investigated the regulatory effect of LncRNA-GAS5 on FDX-1 in HEK293T cells by Acinetobacter baumannii. Transfected LncRNA-GAS5 overexpressing or knocking down plasmids into HEK293T cells, and the expression of FDX-1 was detected by Western blotting. A. baumannii inhibited the expression of FDX-1. Overexpression of LncRNA-GAS5 inhibited the expression of FDX-1, while knocking down LncRNA-GAS5 increased the expression of FDX-1. Overexpression of LncRNA-GAS5 further enhanced the inhibitory effect of A. baumannii on FDX-1, while knocking down LncRNA-GAS5 restored the inhibitory effect of A. baumannii on FDX-1. LncRNA-GAS5 regulates the inhibitory effect of A. baumannii on FDX-1 in HEK293T cells.

References

An Z, Ding W. Acinetobacter baumannii up-regulates LncRNA-GAS5 and promotes the degradation of STX17 by blocking the activation of YY1. Virulence. 2021 Dec;12(1):1965–1979. https://doi.org/10.1080/21505594.2021.1953851
Search in Google Scholar Back to article
An Z, Huang X, Zheng C, Ding W. Acinetobacter baumannii outer membrane protein A induces HeLa cell autophagy via MAPK/JNK signaling pathway. Int J Med Microbiol. 2019a Mar;309(2):97–107. https://doi.org/10.1016/j.ijmm.2018.12.004
Search in Google Scholar Back to article
An Z, Su J. Acinetobacter baumannii outer membrane protein 34 elicits NLRP3 inflammasome activation via mitochondria-derived reactive oxygen species in RAW264.7 macrophages. Microbes Infect. 2019b Apr-May;21(3–4):143–153. https://doi.org/10.1016/j.micinf.2018.10.005
Search in Google Scholar Back to article
Gao Z, Huang D. lncRNA GAS5-mediated miR-23a-3p promotes inflammation and cell apoptosis by targeting TLR4 in a cell model of sepsis. Mol Med Rep. 2021 Jul;24(1):510. https://doi.org/10.3892/mmr.2021.12149
Search in Google Scholar Back to article
Harding CM, Hennon SW, Feldman MF. Uncovering the mechanisms of Acinetobacter baumannii virulence. Nat Rev Microbiol. 2018 Feb;16(2):91–102. https://doi.org/10.1038/nrmicro.2017.148
Search in Google Scholar Back to article
Hu G, Lou Z, Gupta M. The long non-coding RNA GAS5 cooperates with the eukaryotic translation initiation factor 4E to regulate c-Myc translation. PLoS One. 2014 Sep;9(9):e107016. https://doi.org/10.1371/journal.pone.0107016
Search in Google Scholar Back to article
Kino T, Hurt DE, Ichijo T, Nader N, Chrousos GP. Noncoding RNA gas5 is a growth arrest-and starvation-associated repressor of the glucocorticoid receptor. Sci Signal. 2010 Feb;3(107):ra8. https://doi.org/10.1126/scisignal.2000568
Search in Google Scholar Back to article
Li J, Tian J, Lu J, Wang Z, Ling J, Wu X, Yang F, Xia Y. LncRNA GAS5 inhibits Th17 differentiation and alleviates immune thrombocytopenia via promoting the ubiquitination of STAT3. Int Immu-nopharmacol. 2020 Mar;80:106127. https://doi.org/10.1016/j.intimp.2019.106127
Search in Google Scholar Back to article
Li SR, Bu LL, Cai L. Cuproptosis: Lipoylated TCA cycle proteinsmediated novel cell death pathway. Signal Transduct Target Ther. 2022 May;7(1):158. https://doi.org/10.1038/s41392-022-01014-x
Search in Google Scholar Back to article
Li Y, Guo X, Hu C, Du Y, Guo C, Di Wang, Zhao W, Huang G, Li C, Lu Q, et al. Type I IFN operates pyroptosis and necroptosis during multidrug-resistant A. baumannii infection. Cell Death Differ. 2018 Jul;25(7):1304–1318. https://doi.org/10.1038/s41418-017-0041-z
Search in Google Scholar Back to article
Meng H, Zhang T, Wang Z, Zhu Y, Yu Y, Chen H, Chen J, Wang F, Yu Y, Hua X, et al. High-throughput host-microbe single-cell RNA sequencing reveals ferroptosis-associated heterogeneity during Acinetobacter baumannii infection. Angew Chem Int Ed Engl. 2024 Apr;63(18):e202400538. https://doi.org/10.1002/anie.202400538
Search in Google Scholar Back to article
Tsvetkov P, Coy S, Petrova B, Dreishpoon M, Verma A, Abdusamad M, Rossen J, Joesch-Cohen L, Humeidi R, Spangler RD, et al. Copper induces cell death by targeting lipoylated TCA cycle proteins. Science. 2022 Mar;375(6586):1254–1261. https://doi.org/10.1126/science.abf0529
Search in Google Scholar Back to article
Yang X, Xie Z, Lei X, Gan R. Long non-coding RNA GAS5 in human cancer. Oncol Lett. 2020 Sep;20(3):2587–2594. https://doi.org/10.3892/ol.2020.11809
Search in Google Scholar Back to article
Zhang XC, Gu AP, Zheng CY, Li YB, Liang HF, Wang HJ, Tang XL, Bai XX, Cai J. YY1/LncRNA GAS5 complex aggravates cerebral ischemia/reperfusion injury through enhancing neuronal glycolysis. Neuropharmacology. 2019 Nov;158:107682. https://doi.org/10.1016/j.neuropharm.2019.107682
Search in Google Scholar Back to article
Zhao D, Li Y, Peng C, Lin J, Yu F, Zhao Y, Zhang X, Zhao D. Outer membrane protein a in Acinetobacter baumannii induces autophagy through mTOR signalling pathways in the lung of SD rats. Biomed Pharmacother. 2021 Mar;135:111034. https://doi.org/10.1016/j.biopha.2020.111034
Search in Google Scholar Back to article

Knocking Down LncRNA-GAS5 Restores the Inhibition of Cuprotosis Protein FDX-1 by Acinetobacter baumannii

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