Have a personal or library account? Click to login

The Sulfur Conversion Functional Microbial Communities in Biogas Liquid Can Participate in Coal Degradation

Open Access
|Aug 2024

Figures & Tables

Fig. 1.

Flow chart for experimental studies.
Flow chart for experimental studies.

Fig. 2

Changes in microbial diversity in samples. a) Relative abundance of major bacterial phyla in biogas liquid (relative abundance > 1%). b) heatmap for relative abundance of major microbial genera in samples (relative abundance > 0.05%), and red and green arrows indicated significant differences between Treat and CK groups at 90 days (t-text, p < 0.05); c) ordering of microbial community composition by nonmetric multidimensional scaling (NMDS) using the Bray-Curtis distance; d) Shannon diversity among samples, n.s. indicates no significant difference (ANOVA with Tukey’s post hoc test, p < 0.05) among treatments.
Changes in microbial diversity in samples. a) Relative abundance of major bacterial phyla in biogas liquid (relative abundance > 1%). b) heatmap for relative abundance of major microbial genera in samples (relative abundance > 0.05%), and red and green arrows indicated significant differences between Treat and CK groups at 90 days (t-text, p < 0.05); c) ordering of microbial community composition by nonmetric multidimensional scaling (NMDS) using the Bray-Curtis distance; d) Shannon diversity among samples, n.s. indicates no significant difference (ANOVA with Tukey’s post hoc test, p < 0.05) among treatments.

Fig. 3.

Images of SEM, EDS energy spectra, and count per second (cps) data of coal samples from different treatment groups.
Images of SEM, EDS energy spectra, and count per second (cps) data of coal samples from different treatment groups.

Fig. 4.

XRD, FTIR, and XPS spectra of coal samples. a) XRD spectra. b) FTIR spectra. c) XPS spectra. d) XPS spectra of C, N, O, and S, respectively.
XRD, FTIR, and XPS spectra of coal samples. a) XRD spectra. b) FTIR spectra. c) XPS spectra. d) XPS spectra of C, N, O, and S, respectively.

Fig. 5.

Main sulfur-containing organic compounds in coal samples.
Main sulfur-containing organic compounds in coal samples.

Fig. 6.

a) Bacteria in acclimated biogas liquid and functional classes related to DBT degradation; b) sulfur-related intermediates produced by DBT degradation.
a) Bacteria in acclimated biogas liquid and functional classes related to DBT degradation; b) sulfur-related intermediates produced by DBT degradation.

Fig. 7.

Predicted degradation pathways of dibenzothiophene (DBT).
Predicted degradation pathways of dibenzothiophene (DBT).

The relative abundance of dominant phyla (> 1%) in the samples, different lowercase letters indicate a significant difference (ANOVA with Tukey’s post hoc test, p < 0_05) among samples_

Acclimated biogas liquidCK_30dCK_90dTreat_30dTreat_90d
Firmicutes99.23 ± 0.18% a32.75 ± 19.80% b25.11 ± 3.87% b98.32 ± 0.31% a90.65 ± 5.36% a
Proteobacteria0.61 ± 0.12% b63.55 ± 20.87% a67.22 ± 1.41% a1.47 ± 0.38% b2.32 ± 0.69% b
Actinobacteriota0.03 ± 0.02% c3.14 ± 0.95% b5.71 ± 1.51% a0.03 ± 0.02% c0.12 ± 0.06% c
Chloroflexi0.00 ± 0.00% b0.09 ± 0.03% ab0.92 ± 0.85% ab0.09 ± 0.10% ab6.81 ± 5.56% a
Bacteroidota0.07 ± 0.03% a0.37 ± 0.36% a0.42 ± 0.27% a0.04 ± 0.02% a0.03 ± 0.01% a
DOI: https://doi.org/10.33073/pjm-2024-027 | Journal eISSN: 2544-4646 | Journal ISSN: 1733-1331
Language: English
Page range: 315 - 327
Submitted on: Apr 2, 2024
Accepted on: May 29, 2024
Published on: Aug 26, 2024
Published by: Polish Society of Microbiologists
In partnership with: Paradigm Publishing Services
Publication frequency: 4 times per year

© 2024 Yang Li, Zhong Liang, Xinyue Yan, Tianqi Qin, Zhaojun Wu, Chunshan Zheng, published by Polish Society of Microbiologists
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.