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Isolation and Electrochemical Analysis of a Facultative Anaerobic Electrogenic Strain Klebsiella sp. SQ-1 Cover

Isolation and Electrochemical Analysis of a Facultative Anaerobic Electrogenic Strain Klebsiella sp. SQ-1

Polish Journal of Microbiology
Volume 73 (2024): Issue 2 (June 2024)
By: Lei Zhou,  Tuoxian Tang,  Dandan Deng,  Yayue Wang and  Dongli Pei  
Open Access
|Apr 2024

Figures & Tables

Fig. 1.

Enrichment, isolation, and identification of the strain SQ-1 A) The output voltage performance of mixed microflora in a dual-chamber MFC using sodium acetate as a carbon source. Arrows show the supply of sodium acetate. B) The output voltage (circle) and power density (square) curves for mixed microflora. C) Phylogenetic analysis of the strain SQ-1 based on 16S rDNA gene sequences. D) The appearance of the strain SQ-1 and Geobacter sulfurreducens PCA in liquid growth medium after 3 days of growth.

Fig. 2.

Output voltage and power density of Klebsiella sp. SQ-1 in MFC A) The output voltage performance of the strain SQ-1 in a dual-chamber MFC using sodium acetate as a carbon source. Arrows show the supply of sodium acetate. B) The output voltage performance of a model electricigen Geobacter sulfurreducens PCA in a dual-chamber MFC using sodium acetate as a carbon source. Arrows show the supply of sodium acetate. C) The output voltage (circle) and power density (square) curve for the strain SQ-1. D) The output voltage (circle) and power density (square) curves for G. sulfurreducens PCA. E) Comparison of the maximum output voltage of mixed microflora, G. sulfurreducens PCA, and Klebsiella sp. SQ-1 in MFC. F) Comparison of maximum output power density of mixed microflora, G. sulfurreducens PCA, and Klebsiella sp. SQ-1 in MFC.

Fig. 3.

Electrochemical performance of Klebsiella sp. SQ-1 in a three-electrode system A) Chronoamperometrlc curves for the strain SQ-1 In a three-electrode system using sodium acetate as electron acceptors. Inset, cyclic voltammograms of the enriched strain SQ-1 biofilm on electrodes in dltferent stages; a) before blofllm formation; b) after blofllm formation with a current density below 2 μA cm–2. B(1) The morphology of the strain SQ-1 blofllms under the scanning electron microscope (5,000x). Blofllms were attached to the graphite surface. B(2) The appearance of bacterial culture after 5 days of Incubation when the growth medium Is supplemented with lron(III) oxide. A magnet Is placed outside the tube. The arrow shows that the magnet attracts the black suspension. B(3) The Initial appearance of bacterial culture. C) Voltammograms of the strain SQ-1 blofllm. Scan rates: 1, 2, 5, 10, 20, 50 mV s–1. Inset, the linear dependence of the cathodic peak current density at -0.37 V versus scan rates or square roots of scan rates. D) The Nyquist plot for blofllms attached to working electrodes.

Fig. 4.

Ferric reduction of Klebsiella sp. SQ-1 A) The growth curve of strain SQ-1 measured under aerobic and anaerobic conditions. B) The growth curve of strain SQ-1 when the growth medium was supplemented with different amounts of ferric citrate. C) Measurement of reduced Fe2+. D) Analysis of Fe3+ reduction ratio. The concentration of Fe2+ was measured after 30 hours of incubation.

Electrical generation performance of reported electricigens_

StrainsSubstratesVoltage (V)Power density (mW m–A)Current density (μA cm–2)References
Klebsiella sp. SQ-1sodium acetate0.59560625this work
Geobacter sulfurreducens PCAsodium acetate0.47460605(Deng et al. 2015)
Pseudomonas aeruginosaglucose//52.1(Yong et al. 2017)
Klebsiella pneumoniaglucose0.62140.26/(Guo et al. 2020)
Shewanella oneidensislactate/41/(Watson and Logan 2010)
Citrobacter freundiicitrate/204.5/(Huang et al. 2015)
Escherichia coliglucose//34(Qiao et al. 2009)
Rhodoferax ferrireducensglucose//14.6(Liu et al. 2007)
DOI: https://doi.org/10.33073/pjm-2024-013 | Journal eISSN: 2544-4646 | Journal ISSN: 1733-1331
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Language: English
Page range: 143 - 153
Submitted on: Dec 7, 2023
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Accepted on: Feb 26, 2024
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Published on: Apr 27, 2024
Published by: Polish Society of Microbiologists
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
microbial fuel cell, Klebsiella sp.,
electrochemical analysis,
extracellular respiration,
facultative electricigens
Related subjects:
Life sciences,
Microbiology and virology

© 2024 Lei Zhou, Tuoxian Tang, Dandan Deng, Yayue Wang, Dongli Pei, published by Polish Society of Microbiologists
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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