Fig. 1.
Fig. 2.
Fig. 3.
Fig. 4.
Fig. 5.
PCR primers_ Underlined sequences represent the homologous recombinant fragment with the pET28a(+)_
| Primer type | Primer name | Sequences (5′–3′) |
|---|---|---|
| Universal primers | 27F | AGAGTTTGATCCTGGCTCAG |
| 1492R | GGTTACCTTGTTACGACTT | |
| Amplification primersI | 1-Arthrobacter saudimassiliensis-Uox-F | ATGACCGCAACAGAACAGGCG |
| 1-Arthrobacter saudimassiliensis-Uox-R | TCAGCAGAACCCCGCGATGGT | |
| 2-Arthrobacter globiformis-Uox-F | ATGAGCAACAAGATCGTCCTCG | |
| 2-Arthrobacter globiformis-Uox-R | CTAGCAGAAGCCGGCGATGC | |
| 3-Arthrobacter liuii-Uox-F | ATGAGCAGCAAGATCATCCT | |
| 3-Arthrobacter liuii-Uox-R | TTAGCAGAAGCCGGCGATGC | |
| 4-Arthrobacter ulcerisalmonis-Uox-F | ATGAGCAGCAAGATCATCCTC | |
| 4-Arthrobacter ulcerisalmonis-Uox-R | TCAGCAGAATCCGGCGATGC | |
| 5-Arthrobacter rhombi-Uox-F | ATGACTACCACCGCTTCTCAG | |
| 5-Arthrobacter rhombi-Uox-R | TTAGCAGAAGCCTGCGGCAT | |
| 6-Arthrobacter crystallopoietes-Uox-F | ATGACCGCCACCGTGGAATC | |
| 6-Arthrobacter crystallopoietes-Uox-R | GCAGAATCCGGGGATATTTG | |
| 7-Arthrobacter arilaitensis-Uox-F | ATGACTATCGAAACCACCGCC | |
| 7-Arthrobacter arilaitensis-Uox-R | GGCAAATGCCGGGATATTGGA | |
| 8-Arthrobacter dokdonellae-Uox-F | ATCATCCTGGGCGCCAACC | |
| 8-Arthrobacter dokdonellae-Uox-R | GCAGAAGCCTGCGACGCCG | |
| Amplification primersII | TFH-3- Arthrobacter liuii-Uox-F | |
| TFH-3- Arthrobacter liuii-Uox-R |
Effect of different ions and chemical reagents on enzyme activity_
| Chemicals | Final concentration | Residual activity (%) |
|---|---|---|
| Blank | 10 mM | 100.0 |
| K+ | 10 mM | 250.72 ± 2.90 |
| Mg2+ | 10 mM | 279.71 ± 3.34 |
| Fe2+ | 10 mM | 43.48 ± 0.35 |
| Ca2+ | 10 mM | 204.35 ± 4.35 |
| Ni2+ | 10 mM | 181.16 ± 2.90 |
| Co2+ | 10 mM | 17.39 ± 0.33 |
| Ba2+ | 10 mM | 256.52 ± 2.45 |
| Mn2+ | 10 mM | 23.19 ± 0.65 |
| Pb2+ | 10 mM | 256.52 ± 4.35 |
| Zn2+ | 10 mM | 0 |
| EDTA | 10% | 26.09 ± 0.34 |
| SDS | 10% | 0 |
| PSMF | 10% | 53.62 ± 0.79 |