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Prevalence and Biological Characteristics of Listeria Species Isolated from Livestock and Poultry Meat in Gansu Province, China Cover

Prevalence and Biological Characteristics of Listeria Species Isolated from Livestock and Poultry Meat in Gansu Province, China

Polish Journal of Microbiology
Volume 72 (2023): Issue 1 (March 2023)
By: ZHIJIE DONG,  YANAN SUN,  QING CAO,  HONGXIA LIU,  YUANYUAN LIU,  QIHANG CAO,  HUILIN WEI,  CHEN SONG,  HUITIAN GOU and  HUIWEN XUE  
Open Access
|Mar 2023

Figures & Tables

Fig. 1.

Distribution diagram of sampling points in Gansu province, China. The geographic locations of samples from slaughterhouses and markets. The five-pointed stars of red represent the different sampling sites.
Distribution diagram of sampling points in Gansu province, China. The geographic locations of samples from slaughterhouses and markets. The five-pointed stars of red represent the different sampling sites.

Fig. 2.

Serogroups by multiplex-PCR of L. monocytogenes isolates. M – DL 2000 DNA Maker, 1 – ATCC® 19115™ (serogroup 4b), 2 – ATCC® 19111™ (serogroup 1/2a), 3 – NCTC10890 (serogroup 1/2b), 4 – ATCC® 19112™ (serogroup 1/2c), 5–18 – the strains isolated in this study, 19 – L. innocua (negative control)
Serogroups by multiplex-PCR of L. monocytogenes isolates. M – DL 2000 DNA Maker, 1 – ATCC® 19115™ (serogroup 4b), 2 – ATCC® 19111™ (serogroup 1/2a), 3 – NCTC10890 (serogroup 1/2b), 4 – ATCC® 19112™ (serogroup 1/2c), 5–18 – the strains isolated in this study, 19 – L. innocua (negative control)

Fig. 3.

The growth curves of the Listeria monocytogenes isolates. Each data point is the average of triplicates, and the standard deviations are indicated as error bars. a) The growth curves for four serogroups of L. monocytogenes and one reference strain ATCC® 19111™, b) the growth curves for three isolates of L. innocua, c) the growth curves for three isolates of L. welshimeri.
The growth curves of the Listeria monocytogenes isolates. Each data point is the average of triplicates, and the standard deviations are indicated as error bars. a) The growth curves for four serogroups of L. monocytogenes and one reference strain ATCC® 19111™, b) the growth curves for three isolates of L. innocua, c) the growth curves for three isolates of L. welshimeri.

Fig. 4.

Biofilm formation by Listeria spp. isolates. The absorbance at 562 nm was measured for 22 L. innocua, 10 L. welshimeri, and 14 L. monocytogenes biofilms.
Biofilm formation by Listeria spp. isolates. The absorbance at 562 nm was measured for 22 L. innocua, 10 L. welshimeri, and 14 L. monocytogenes biofilms.

Fig. 5.

Cluster analysis of Listeria spp. isolates based on the sequences of the 16S rRNA genes. The tree was calculated using the Neighbor-Joining method. Isolates isolated in this study are indicated with circles (L. monocytogenes), boxes (L. innocua), and triangles (L. welshimeri). The scale bar represents 0.005 nucleotide substitutions per character. The numbers in the tree indicate the significance (percent of outcomes) of the branches (bootstrap analysis).
Cluster analysis of Listeria spp. isolates based on the sequences of the 16S rRNA genes. The tree was calculated using the Neighbor-Joining method. Isolates isolated in this study are indicated with circles (L. monocytogenes), boxes (L. innocua), and triangles (L. welshimeri). The scale bar represents 0.005 nucleotide substitutions per character. The numbers in the tree indicate the significance (percent of outcomes) of the branches (bootstrap analysis).

Primer information_

Gene targetPrimer sequence (5’-3’)Product size(bp)Application
prs-FGCTGAAGAGATTGCGAAAGAAG370Listeria species-specific primers
prs-RCAAAGAAACCTTGGATTTGCGG
Lmo2234-FTGTCCAGTTCCATTTTTAACT420Listeria monocytogenes specific primers
Lmo2234-RTTGTTGTTCTGCTGTACGA
Lin0464-FCGCATTTATCGCCAAAACTC749Listeria innocua specific primers
Lin0464-RTCGTGACATAGACGCGATTG
lmo0737-FAGGGCTTCAAGGACTTACCC691For the identification of Listeria monocytogenes serogroup
lmo0737-RACGATTTCTGCTTGCCATTC
lmo1118-FAGGGGTCTTAAATCCTGGAA909For the identification of Listeria monocytogenes serogroup
lmo1118-RCGGCTTGTTCGGCATACTTA
ORF2819-FAGCAAAATGCCAAAACTCGT471For the identification of Listeria monocytogenes serogroup
ORF2819-RCATCACTAAAGCCTCCCATTG
ORF2110-FAGTGGACAATTGATTGGTGAA597For the identification of Listeria monocytogenes serogroup
ORF2110-RCATCCATCCCTTACTTTGGAC
16S rRNA-27FAGAGTTTGATCCTGGCTCAG1,500Used for cluster analysis
16S rRNA-1492RGGTTACCTTGTTACGACTT

Statistic of data on the prevalence of Listeria spp_ according to regions and sample categories_

Number of samplesListeria monocytogenes positive samples (%)Listeria innocua positive samples (%)Listeria welshimeri positive samples (%)Listeria spp. positive samples (%)
Different regions
Lanzhou City2987 (2.4a)30 (10.1b)2 (0.7b)39 (13.1b)
Qingyang City2756 (2.2b)95 (34.6a)4 (1.5a)105 (38.2a)
Jiuquan City1291 (0.8c)2 (1.6d)0 (0c)3 (2.3d)
Dingxi City4000 (0d)6 (1.5d)0 (0c)6 (1.5e)
Zhangye City2850 (0d)17 (6.0c)4 (1.4a)21 (7.4c)
Total            1,38714 (1.0)150 (10.8)10 (0.7)174 (12.6)
Different categories of samples
Pork7848 (1.0b)81 (10.3d)7 (0.9a)96 (12.3c)
Beef and mutton2983 (1.0b)35 (11.7b)2 (0.7b)40 (13.4a)
Chicken2643 (1.14a)29 (11.0c)1 (0.4c)33 (12.5b)
Environment samples410 (0b)5 (12.2a)0 (0d)5 (12.2c)
Total            1,38714 (1.0)150 (10.8)10 (0.7)174 (12.6)

Result of drug susceptibility of Listeria spp_ isolates_

AntibioticsListeria monocytogenesListeria innocuaListeria welshimeri
Resistant isolatesIntermediate isolatesSensitive isolatesResistance rates (%)Resistant isolatesIntermediate isolatesSensitive isolatesResistance rates (%)Resistant isolatesIntermediate isolatesSensitive isolatesResistance rates (%)
Penicillin13  0  1  92.9  2  020    9.1  3  0  7  30.0
Ofloxacin  1  211    7.1  113  8    4.6  2  0  8  20.0
Cefoxitin14  0  0100    0  022  0  2  0  8  20.0
Sulfamethoxazole11  0  3  78.610  111  45.5  4  0  6  40.0
Tetracycline14  0  0100  14  0  8  63.6  4  0  6  40.0
Gentamicin  3  011  21.4  0  022  0  1  0  9  10.0
Streptomycin  2  111  14.3  5  017  23.7  1  0  9  10.0
Erythromycin13  1  0  92.9  4  414  18.2  3  1  6  30.0
Acetylspiramycin13  0  1  92.9  5  017  22.7  3  0  7  30.0
Fosfomycin  7  7  0  50.018  0  4  81.8  4  2  4  40.0
DOI: https://doi.org/10.33073/pjm-2023-002 | Journal eISSN: 2544-4646 | Journal ISSN: 1733-1331
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Language: English
Page range: 11 - 20
Submitted on: Oct 6, 2022
Accepted on: Dec 27, 2022
Published on: Mar 24, 2023
Published by: Polish Society of Microbiologists
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
livestock and poultry meat,
slaughtering,
cluster analysis
Related subjects:
Life sciences,
Microbiology and virology

© 2023 ZHIJIE DONG, YANAN SUN, QING CAO, HONGXIA LIU, YUANYUAN LIU, QIHANG CAO, HUILIN WEI, CHEN SONG, HUITIAN GOU, HUIWEN XUE, published by Polish Society of Microbiologists
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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