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Breeding of High Daptomycin-Producing Strain by Streptomycin Resistance Superposition Cover

Breeding of High Daptomycin-Producing Strain by Streptomycin Resistance Superposition

Polish Journal of Microbiology
Volume 71 (2022): Issue 3 (September 2022)
By: Shuaibei Chu,  Wenting Hu,  Kaihong Zhang and  Fengli Hui  
Open Access
|Sep 2022

Figures & Tables

Fig. 1

The growth of Streptomyces roseosporus original strain on DT plates with a) 0 μg/ml, b) 0.8 μg/ml, c) 1.0 μg/ml, d) 1.2 μg/ml, e) 1.4 μg/ml streptomycin, respectively.
The growth of Streptomyces roseosporus original strain on DT plates with a) 0 μg/ml, b) 0.8 μg/ml, c) 1.0 μg/ml, d) 1.2 μg/ml, e) 1.4 μg/ml streptomycin, respectively.

Fig. 2

Daptomycin production by Streptomyces roseosporus mutants screened by DT plates with streptomycin.at a concentration of a) 6 μg/ml, b) 1.8 μg/ml, c) 2.0 μg/ml, d) 2.2 μg/ml, e) 2.4 μg/ml, f) 2.6 μg/ml, g) 2.8 μg/ml, and h) 3.0 μg/ml. The dashed line represents daptomycin production of the parent strain.
Daptomycin production by Streptomyces roseosporus mutants screened by DT plates with streptomycin.at a concentration of a) 6 μg/ml, b) 1.8 μg/ml, c) 2.0 μg/ml, d) 2.2 μg/ml, e) 2.4 μg/ml, f) 2.6 μg/ml, g) 2.8 μg/ml, and h) 3.0 μg/ml. The dashed line represents daptomycin production of the parent strain.

Fig. 3

a1) and b1) Colony and individual morphology of Streptomyces roseosporus strains BNCC 342432 and SR-2620; a2) and b2) spore growth capacity of BNCC 342432 and SR-2620; a3) and b3) mycelial pellet morphology of BNCC 342432 and SR-2620.
a1) and b1) Colony and individual morphology of Streptomyces roseosporus strains BNCC 342432 and SR-2620; a2) and b2) spore growth capacity of BNCC 342432 and SR-2620; a3) and b3) mycelial pellet morphology of BNCC 342432 and SR-2620.

Fig. 4

Sequence comparison of the rpsL gene between Streptomyces roseosporus BNCC 342432 and SR-2620 strains.
Sequence comparison of the rpsL gene between Streptomyces roseosporus BNCC 342432 and SR-2620 strains.

Fig. 5

Comparison of shake flask fermentation between Streptomyces roseosporus BNCC 342432 and SR-2620 strains.
Comparison of shake flask fermentation between Streptomyces roseosporus BNCC 342432 and SR-2620 strains.

Stability experiment on high-yield daptomycin strains_

StrainFirst generation of daptomycin production (mg/l)Second generation of daptomycin production (mg/l)Third generation of daptomycin production (mg/l)Fourth generation of daptomycin production (mg/l)Fifth generation of daptomycin production (mg/l)
BNCC 34243210.5 ± 0.29.9 ± 0.210.3 ± 0.29.7 ± 0.210.4 ± 0.2
SR-200928.5 ± 0.230.0 ± 0.224.3 ± 0.225.0 ± 0.222.8 ± 0.2
SR-201723.1 ± 0.221.6 ± 0.222.8 ± 0.219.8 ± 0.214.6 ± 0.2
SR-202329.7 ± 0.228.8 ± 0.230.6 ± 0.229.2 ± 0.227.2 ± 0.2
SR-202624.2 ± 0.222.9 ± 0.221.8 ± 0.220.1 ± 0.220.6 ± 0.2
SR-240124.5 ± 0.225.5 ± 0.223.4 ± 0.222.5 ± 0.224.2 ± 0.2
SR-262038.5 ± 0.236.9 ± 0.241.3 ± 0.236.0 ± 0.238.5 ± 0.2
DOI: https://doi.org/10.33073/pjm-2022-041 | Journal eISSN: 2544-4646 | Journal ISSN: 1733-1331
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Language: English
Page range: 463 - 471
Submitted on: May 5, 2022
Accepted on: Aug 2, 2022
Published on: Sep 24, 2022
Published by: Polish Society of Microbiologists
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
daptomycin,
streptomycin resistance,
strain breeding,
fermentation
Related subjects:
Life sciences,
Microbiology and virology

© 2022 Shuaibei Chu, Wenting Hu, Kaihong Zhang, Fengli Hui, published by Polish Society of Microbiologists
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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