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Detection by Whole-Genome Sequencing of a Novel Metallo-β-Lactamase Produced by Wautersiella falsenii Causing Urinary Tract Infection in Tunisia Cover

Detection by Whole-Genome Sequencing of a Novel Metallo-β-Lactamase Produced by Wautersiella falsenii Causing Urinary Tract Infection in Tunisia

Open Access
|Feb 2022

Figures & Tables

Fig. 1

Phylogenetic tree based on the protein sequences of; serine β-lactamase (Class A and Class C) and metallo-β-lactamase (Class B).
Phylogenetic tree based on the protein sequences of; serine β-lactamase (Class A and Class C) and metallo-β-lactamase (Class B).

Fig. 2

Protein alignment among the metallo-β-lactamase type B genes: Our sequence was represented with red color. The conserved motif of the MBL “HxHxDH” presented in their active site, and residues of bacterial metallo-β-lactamase enzymes were represented with a yellow color.
Protein alignment among the metallo-β-lactamase type B genes: Our sequence was represented with red color. The conserved motif of the MBL “HxHxDH” presented in their active site, and residues of bacterial metallo-β-lactamase enzymes were represented with a yellow color.

Comparison between all reported Wautersiella falsenii bacterial infections and that of the present study_

ReferenceNumber of isolatesYears of isolationCountryGender and age of infected patientsPatient suffered fromClinical specimensPhenotypic resistance patternDetected genesDetected plasmids
Kämpfer et al. 2006261980–2004Belgiumblood, wounds, pus, respiratory tract, ear discharge, oral cavity, vaginal swab, pleural fluid, and other from an unknown origin
Van der Velden et al. 201212012Netherlandsone-year-old girlpyelonephritisurinenitrofurantoin, amoxicillin-clavulanic acid, piperacillin-tazobactam, ceftriaxone, ceftazidime, meropenem, tobramycin, and colistin
Traglia et al. 201512013Argentina18-year-old womanacute otitis mediacervical neck abscessampicillin, ampicillin-sulbactam, cephalothin, meropenem, colistin29 genes coding for efflux pumps, and tripartite multidrug resistance systems, class a β-lactamase, metallo-β-lactamase (ebr-2), three class c β-lactamases, resistance to streptogramin trimethoprim, bacitracin, and macrolidenone
Giordano et al. 201612016Italy32-year-old manlymphoblastic leukemiathe respiratory tractamikacin, amikacin-clavulanic acid, ampicillin-sulbactam, cefotaxime, ceftazidime, doripenem, gentamicin, imipenem, piperacillin-tazobactam
Zaman et al. 201712017Indiafive-year-old boybladder cancerurineampicillin, ampicillin-sulbactam, and colistin.
This study12016Tunisia35-year-old womanUTIurineamoxicillin, amoxicillin-clavulanic acid, ticarcillin, ticarcillin-clavulanic acid, amikacin, tobramycin, streptomycin, spectinomycin, ciprofloxacin, pefloxacin, nalidixic acid, furans, nitrofurantoinblaEBR-like, TetX and aadSnone
DOI: https://doi.org/10.33073/pjm-2022-010 | Journal eISSN: 2544-4646 | Journal ISSN: 1733-1331
Language: English
Page range: 73 - 81
Submitted on: Aug 20, 2021
Accepted on: Nov 22, 2021
Published on: Feb 27, 2022
Published by: Polish Society of Microbiologists
In partnership with: Paradigm Publishing Services
Publication frequency: 4 times per year

© 2022 Raouaa Maaroufi, Olfa Dziri, Linda Hadjadj, Seydina M. Diene, Jean-Marc Rolain, Chedly Chouchani, published by Polish Society of Microbiologists
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.