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A Salt-Tolerant Streptomyces paradoxus D2-8 from Rhizosphere Soil of Phragmites communis Augments Soybean Tolerance to Soda Saline-Alkali Stress Cover

A Salt-Tolerant Streptomyces paradoxus D2-8 from Rhizosphere Soil of Phragmites communis Augments Soybean Tolerance to Soda Saline-Alkali Stress

Polish Journal of Microbiology
Volume 71 (2022): Issue 1 (March 2022)
By:
Open Access
|Mar 2022

Figures & Tables

Fig. 1

a) Stress tolerance of strain D2-8 in different concentration of salt and b) saline-alkali solutions. SA represents saline and alkaline solution, which contains NaCl, Na2CO3, NaHCO3, and Na2SO4 (molar ratio 1:1:9:9).
a) Stress tolerance of strain D2-8 in different concentration of salt and b) saline-alkali solutions. SA represents saline and alkaline solution, which contains NaCl, Na2CO3, NaHCO3, and Na2SO4 (molar ratio 1:1:9:9).

Fig. 2

Morphological characteristics of strain D2-8 observed by scanning electron microscopy.
Morphological characteristics of strain D2-8 observed by scanning electron microscopy.

Fig. 3

Neighbor-joining tree of strain D2-8 and the related species of the genus Streptomyces based on the 16S rRNA gene sequences. The out-group used was Mycobacterium tuberculosis H37RvT. The stability of the topology of the phylogenetic tree was assessed by the bootstrap method with 1000 repetitions. Only bootstrap values above 50% (percentages of 1000 replications) are indicated. Scale bar represents 0.01 nucleotide substitutions per site.
Neighbor-joining tree of strain D2-8 and the related species of the genus Streptomyces based on the 16S rRNA gene sequences. The out-group used was Mycobacterium tuberculosis H37RvT. The stability of the topology of the phylogenetic tree was assessed by the bootstrap method with 1000 repetitions. Only bootstrap values above 50% (percentages of 1000 replications) are indicated. Scale bar represents 0.01 nucleotide substitutions per site.

Fig. 4

a) The distribution of subsystems of the D2-8 genome annotated through the RAST webserver and b) stress response genes of D2-8 involved in the production and uptake of choline and glycine betaine.
a) The distribution of subsystems of the D2-8 genome annotated through the RAST webserver and b) stress response genes of D2-8 involved in the production and uptake of choline and glycine betaine.

Fig. 5

The growth promotion effect of D2-8 under normal conditions (a, b, c) and saline-alkali stress (d, e, f) on soybean. For a), b), c), control group: water treatment, 108 D2-8: the D2-8 spore concentration of 108 CFU/ml; 107 D2-8: the D2-8 spore concentration of 107 CFU/ml; 106 D2-8: the D2-8 spore concentration of 106 CFU/ml. For d), e), f), control group: normal condition, SA group: saline-alkali stress treatment, SA+D2-8: D2-8 treatment under saline-alkali stress condition; Plants height, roots length, and fresh weight of roots and aboveground were analyzed using unpaired t-tests (n = 15 plants). The symbols * and ** indicate p values 0.05 and 0.01.
The growth promotion effect of D2-8 under normal conditions (a, b, c) and saline-alkali stress (d, e, f) on soybean. For a), b), c), control group: water treatment, 108 D2-8: the D2-8 spore concentration of 108 CFU/ml; 107 D2-8: the D2-8 spore concentration of 107 CFU/ml; 106 D2-8: the D2-8 spore concentration of 106 CFU/ml. For d), e), f), control group: normal condition, SA group: saline-alkali stress treatment, SA+D2-8: D2-8 treatment under saline-alkali stress condition; Plants height, roots length, and fresh weight of roots and aboveground were analyzed using unpaired t-tests (n = 15 plants). The symbols * and ** indicate p values 0.05 and 0.01.

The secondary metabolism substances prediction results of antiSMASH_

RegionTypeFromToMost similar known clusterSimilarity
Region 1.1siderophore27,76639,535desferrioxamin B / desferrioxamine Eother83%
Region 1.2melanin, lanthipeptide125,173149,340melaninother80%
Region 23.1melanin99,231109,596melaninother57%
Region 1.3terpene, NRPS542,592599,827SCO-2138RiPP85%
Region 8.1terpene65,77690,277isorenierateneterpene100%
Region 8.2terpene227,330248,343albaflavenoneterpene100%
Region 3.1terpene232,223258,904hopeneterpene92%
Region 9.2terpene221,324243,489geosminterpene100%
Region 17.2terpene157,127178,023CC-1065other24%
Region 28.1terpene3,61324,626A23187 calcimycinpolyketide10%
Region 31.1terpene11,83732,925rustmicinpolyketide: iterative type I10%
Region 2.1ectoine76,35986,757ectoineother100%
Region 3.2NRPS364,532415,460coelichelinNRP100%
Region 36.1NRPS135,822(2S,6R)-diamino-(5R,7)-dihydroxy-heptanoic acidNRP24%
Region 3.4bacteriocin, lanthipeptide786,397813,467informatipeptinRiPP: lanthipeptide100%
Region 5.1PKS-like, butyrolactone18,66871,723ulleungmycinNRP8%
Region 7.2LAP, thiopeptide331,828365,008diazepinomicinterpene7%
Region 3.5T1PKS, T3PKS874,824939,794alkylresorcinolpolyketide100%
Region 9.4T1PKS400,230434,396mediomiycin Apolyketide36%
Region 12.1T1PKS124,057argimycin PI / argimycin PII / nigrifactin / argimycin PIV / argimycin PV / argimycin PVI / argimycin PIXpolyketide: modular type I21%
Region 17.1T2PKS79,071151,586spore pigmentpolyketide83%
Region 39.1T2PKS125,935collinomycinpolyketide72%
Region 33.1T3PKS8,16849,481germicidinother100%

Physiological characteristics of strain D2-8_

CharacteristicD2-8CharacteristicD2-8CharacteristicD2-8
Carbon source utilizationNitrogen source utilizationMelanin formation on peptone-iron agar
Lucose+L-threonine+Cellulose decomposition–
Arabinose+L-tyrosine+Enzymatic activity
Sucrose−L-asparagine+Protease+
Xylose+Alanine+Liquefaction of gelatin+
Inositol+Glycine+Production of H2S+
Mannitol+L-glutaminealanine+
Fructose−L-arginine−
Rhamnose−L-proline+
Raffinose+
Lactose+
D-ribose++ − positive,
D-galactose+− − negative

Plant growth promotion properties of strain D2-8_

Characteristic of D2-8Value or properties
IAA production29.1 μg/ml
siderophore production+
organic acids production+
1-aminocyclopropane-1-carboxylate deaminase (ACCD) production+

Effect of Streptomyces D2-8 on the soybean production in the field experiment_

TreatmentDensity (Ten thousand/ha)Plant height (cm)Number of effective sectionsPod number per plantGrain number per plant100 grains weight (g)Yield (kg/ha)
D2-833.85 ± 0.76*92.30 ± 4.0212.40 ± 0.3725.45 ± 1.7555.95 ± 1.6819.51 ± 0.49*2463.85 ± 56.36**
Control31.54 ± 1.2893.95 ± 2.4211.90 ± 0.3524.45 ± 0.5457.60 ± 2.7618.73 ± 0.292327.85 ± 42.46
DOI: https://doi.org/10.33073/pjm-2022-006 | Journal eISSN: 2544-4646 | Journal ISSN: 1733-1331
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Language: English
Page range: 43 - 53
Submitted on: Dec 10, 2021
Accepted on: Jan 21, 2022
Published on: Mar 14, 2022
Published by: Polish Society of Microbiologists
In partnership with: Paradigm Publishing Services
Publication frequency: 4 times per year
Keywords:
Actinomycetes,
rhizosphere,
soda saline-alkali tolerance,
plant growth promotion
Related subjects:
Life sciences,
Microbiology and virology

© 2022 Yamei Gao, Yiqiang Han, Xin Li, Mingyang Li, Chunxu Wang, Zhiwen Li, Yanjie Wang, Weidong Wang, published by Polish Society of Microbiologists
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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