Have a personal or library account? Click to login
Rapid Detection and Differentiation of KPC and MBL Carbapenemases among Enterobacterales Isolates by a Modified Combined-Disk Test Cover

Rapid Detection and Differentiation of KPC and MBL Carbapenemases among Enterobacterales Isolates by a Modified Combined-Disk Test

Polish Journal of Microbiology
Volume 70 (2021): Issue 3 (September 2021)
By: MING WEI,  PENG WANG,  SHUAI WANG,  CHUNXIA YANG and  LI GU  
Open Access
|Sep 2021

Figures & Tables

Fig. 1.

The inhibitory zone diameter distribution of a) imipenem, b) meropenem for Enterobacterales isolates tested.
The inhibitory zone diameter distribution of a) imipenem, b) meropenem for Enterobacterales isolates tested.

Fig. 2.

Flow diagram of the mCDT for distinguishing CRE from CSE isolates, and differentiating KPC and/or MBL producers.A – imipenem disk alone, B – imipenem disk plus 5 µl EDTA, C – imipenem disk plus 10 µl APB and D – imipenem disk plus 5 µl EDTA and 10 µl APB.
Flow diagram of the mCDT for distinguishing CRE from CSE isolates, and differentiating KPC and/or MBL producers.A – imipenem disk alone, B – imipenem disk plus 5 µl EDTA, C – imipenem disk plus 10 µl APB and D – imipenem disk plus 5 µl EDTA and 10 µl APB.

Fig. 3.

Examples of mCDT showing the inhibitory zone diameters of A, B, C, and D for a) a plate 7, 7, 14, and 15 mm confirming the KPC-producing isolate, and for b) a plate 9, 17, 8, 18 mm confirming the MBL-producing isolate.A – imipenem disk alone, B – imipenem disk plus 5 µl EDTA, C – imipenem disk plus 10 µl APB and D – imipenem disk plus 5 µl EDTA and 10 µl APB.
Examples of mCDT showing the inhibitory zone diameters of A, B, C, and D for a) a plate 7, 7, 14, and 15 mm confirming the KPC-producing isolate, and for b) a plate 9, 17, 8, 18 mm confirming the MBL-producing isolate.A – imipenem disk alone, B – imipenem disk plus 5 µl EDTA, C – imipenem disk plus 10 µl APB and D – imipenem disk plus 5 µl EDTA and 10 µl APB.

Species distribution of different carbapenemase types among non-CP-CRE and 3GCeRE isolates_

Category (n)Species
KpnEcoEclKaeCfrPrePmiSma
KPC (107)104    1    0    11000
MBL (36)
NDM (30)    7  15    1    01411
IMP (6)    3    0    3    00000
OXA-48-like (1)    1    0    0    00000
KPC+NDM (1)    1    0    0    00000
Non-CP-CRE (18)    8    5    2    30000
3GCeRE (90)  17  47    7  102142
Total (253)141  68  13  144553

Accuracy of detecting KPC or MBL carbapenemases among 163 CRE isolates under different conditions_

CategoryCarbapenemaseAccuracy
Sensitivity (%), (95% CI)Specificity (%), (95% CI)
IPM-5 μ APBKPC88.8 (80.9-93.8)100 (92.0-100)
IPM-5 μ EDTAMBL100 (88.0-100)100 (96.3-100)
IPM-10 μ APBKPC97.2 (91.4-99.3)100 (92.0-100)
IPM-10 μ EDTAMBL100 (88.0-100)100 (96.3-100)
MEM-5 μ APBKPC48.6 (38.9-58.4)100 (92.0-100)
MEM-5 μ EDTAMBL100 (88.0-100)100 (96.3-100)
MEM-10 μ APBKPC59.8 (49.9-69.0)100 (92.0-100)
MEM-10 μ EDTAMBL100 (88.0-100)100 (96.3-100)
DOI: https://doi.org/10.33073/pjm-2021-036 | Journal eISSN: 2544-4646 | Journal ISSN: 1733-1331
Journal RSS Feed
Language: English
Page range: 387 - 394
Submitted on: May 13, 2021
Accepted on: Jul 28, 2021
Published on: Sep 17, 2021
Published by: Polish Society of Microbiologists
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
carbapenem-resistant Enterobacterales,
carbapenemase,
rapid detection and differentiation,
modified combined-disk test
Related subjects:
Life sciences,
Microbiology and virology

© 2021 MING WEI, PENG WANG, SHUAI WANG, CHUNXIA YANG, LI GU, published by Polish Society of Microbiologists
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Previous articleVolume 70 (2021): Issue 3 (September 2021)Next article