Fig. 1.
Fig. 2.
Fig. 3.
The compounds identified in three solvents extracts from the alkali lignin degraded by the bacterial strains and the control sample_
| RT (min) | Compounds | CK | YB5 | LN4 | LN2 | CL32 |
|---|---|---|---|---|---|---|
| Hexane as solvent | ||||||
| 19.24 | Pentonic acid lactone* | – | + | + | + | – |
| 22.65 | Hexadecanoate* | – | + | + | + | + |
| 24.46 | Octadecanoate* | – | + | + | + | + |
| 28.20 | Propyl hexadecanoate* | – | + | + | + | + |
| Dioxane as solvent | ||||||
| 8.45 | 2-Ethoxyethanol | + | + | + | + | + |
| 11.67 | 2,4-Hexadienal | + | + | + | + | + |
| 12.83 | Di (ethylene glycol) vinyl ether | + | + | + | + | + |
| 21.92 | Dimethylbiphenyl | + | – | – | – | – |
| 24.07 | 1,2,3-trimethyl-4-prop-1-enylnaphthalene | + | – | – | – | – |
| 26.87 | Diisobutyl phthalate | + | – | – | – | – |
| 29.32 | Dibutyl phthalate | + | – | – | – | – |
| Ethyl acetate as solvent | ||||||
| 21.54 | Acetosyringone | + | + | + | + | + |
| 22.93 | Diisooctyl phthalate | + | – | – | – | – |
| 23.6 | Palmitoleic acid | + | – | – | – | – |
| 23.79 | Dibutyl phthalate | + | – | – | – | – |
| 25.51 | Cis-Vaccenic acid | + | – | – | – | – |
Screening and identification of bacterial isolates based on different media_
| Name | Species | Accession number | Alkaline lignin | Aniline blue | Brilliant blue |
|---|---|---|---|---|---|
| YB5 | Bacillus aryabhattai | MT745877 | ∘ | + | – |
| CL32 | Micrococcus yunnanensis | MT745880 | ∘ | + | – |
| LN2 | Acinetobacter johnsonii | MT745878 | ∘ | ++ | – |
| LN4 | Acinetobacter lwoffii | MT745879 | ∘ | ++ | – |