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In vitro Antagonistic Activity of Endophytic Fungi Isolated from Shirazi Thyme (Zataria multiflora Boiss.) against Monosporascus cannonballus Cover

In vitro Antagonistic Activity of Endophytic Fungi Isolated from Shirazi Thyme (Zataria multiflora Boiss.) against Monosporascus cannonballus

Open Access
|Aug 2020

Figures & Tables

Fig. 1.

In vitro growth inhibition of Monosporascus cannonballus after dual cultivation with several endophytic fungi from Zataria multiflora.a) M. cannonballus (Mc) alone; b) M. cannonballus + N. sphaerica E1; c) M. cannonballus + N. sphaerica E6; d) M. cannonballus + S. cristata E7; e) M. cannonballus + Paecilomyces sinensis E8; f) M. cannonballus + P. sinensis E10
In vitro growth inhibition of Monosporascus cannonballus after dual cultivation with several endophytic fungi from Zataria multiflora.a) M. cannonballus (Mc) alone; b) M. cannonballus + N. sphaerica E1; c) M. cannonballus + N. sphaerica E6; d) M. cannonballus + S. cristata E7; e) M. cannonballus + Paecilomyces sinensis E8; f) M. cannonballus + P. sinensis E10

Fig. 2.

Scanning electron micrograph showing morphological changes in the hyphae of Monosporascus cannonballus at the edge of the inhibition zone after co-cultivation with Nigrospora sphaerica E1 in PDA platesa) Hyphae of M. cannonballus in the control; b) Hyphae of M. cannonballus after co-cultivation with N. sphaerica E1.
Scanning electron micrograph showing morphological changes in the hyphae of Monosporascus cannonballus at the edge of the inhibition zone after co-cultivation with Nigrospora sphaerica E1 in PDA platesa) Hyphae of M. cannonballus in the control; b) Hyphae of M. cannonballus after co-cultivation with N. sphaerica E1.

Percentage inhibition of mycelial growth of M_ cannonballus by endophytic fungi isolated from Zataria multiflora in dual cultures on PDA_

Fungal endophyte% Inhibition
Nigrospora sphaerica E181.7 (64.7) ± 5.5a
Nigrospora sphaerica E666.1 (54.4) ± 1.9a
Subramaniula cristata E738.7 (38.5) ± 3.7b
Polycephalomyces sinensis E880.6 (63.9) ± 11.2a
Polycephalomyces sinensis E1075.8 (60.5) ± 9.3a

Electrolyte leakage induced by culture filtrates of endophytic fungi from the mycelium of M_ cannonballus_

TreatmentsElectrical conductivity (mS cm–1)
0 min1 h3 h
Nigrospora sphaerica E13.95 ± 0.02a3.98 ± 0.02a4.12 ± 0.06a
Nigrospora sphaerica E63.90 ± 0.02b3.87 ± 0.02a4.01 ± 0.04b
Subramaniula cristata E73.46 ± 0.00c3.41 ± 0.01c3.55 ± 0.01d
Polycephalomyces sinensis E83.10 ± 0.03d3.28 ± 0.14c3.14 ± 0.01e
Polycephalomyces sinensis E103.50 ± 0.02c3.61 ± 0.00b3.71 ± 0.01c
Czapek Dox broth (un inoculated)2.01 ± 0.00e2.01 ± 0.00d2.08 ± 0.00f
Control (water)0.65 ± 0.01f0.67 ± 0.00e0.71 ± 0.01g
DOI: https://doi.org/10.33073/pjm-2020-029 | Journal eISSN: 2544-4646 | Journal ISSN: 1733-1331
Language: English
Page range: 379 - 383
Submitted on: Mar 23, 2020
Accepted on: Jun 18, 2020
Published on: Aug 5, 2020
Published by: Polish Society of Microbiologists
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year

© 2020 RAHIL SAID AL-BADI, THAMODINI GAYA KARUNASINGHE, ABDULLAH MOHAMMED AL-SADI, ISSA HASHIL AL-MAHMOOLI, RETHINASAMY VELAZHAHAN, published by Polish Society of Microbiologists
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