In vitro Antagonistic Activity of Endophytic Fungi Isolated from Shirazi Thyme (Zataria multiflora Boiss.) against Monosporascus cannonballus

Fungal endophyte	% Inhibition
Nigrospora sphaerica E1	81.7 (64.7) ± 5.5^a
Nigrospora sphaerica E6	66.1 (54.4) ± 1.9^a
Subramaniula cristata E7	38.7 (38.5) ± 3.7^b
Polycephalomyces sinensis E8	80.6 (63.9) ± 11.2^a
Polycephalomyces sinensis E10	75.8 (60.5) ± 9.3^a

1 Data are mean of four replications ± standard deviation. Figures in parentheses are arc sine transformed values. Values in columns followed by different letters indicate significant differences according to the Tukey’s test (p < 0.05).

*In vitro* growth inhibition of *Monosporascus cannonballus* after dual cultivation with several endophytic fungi from *Zataria multiflora*.
a) *M. cannonballus* (Mc) alone; b) *M. cannonballus* + *N. sphaerica* E1; c) *M. cannonballus* + *N. sphaerica* E6; d) *M. cannonballus* + *S. cristata* E7; e) *M. cannonballus* + *Paecilomyces sinensis* E8; f) *M. cannonballus* + *P. sinensis* E10

Scanning electron micrograph showing morphological changes in the hyphae of *Monosporascus cannonballus* at the edge of the inhibition zone after co-cultivation with *Nigrospora sphaerica* E1 in PDA plates
a) Hyphae of *M. cannonballus* in the control; b) Hyphae of *M. cannonballus* after co-cultivation with *N. sphaerica* E1.

Table II

Electrolyte leakage induced by culture filtrates of endophytic fungi from the mycelium of M. cannonballus.

Treatments	Electrical conductivity (mS cm^–1)
Treatments	0 min	1 h	3 h
Nigrospora sphaerica E1	3.95 ± 0.02^a	3.98 ± 0.02^a	4.12 ± 0.06^a
Nigrospora sphaerica E6	3.90 ± 0.02^b	3.87 ± 0.02^a	4.01 ± 0.04^b
Subramaniula cristata E7	3.46 ± 0.00^c	3.41 ± 0.01^c	3.55 ± 0.01^d
Polycephalomyces sinensis E8	3.10 ± 0.03^d	3.28 ± 0.14^c	3.14 ± 0.01^e
Polycephalomyces sinensis E10	3.50 ± 0.02^c	3.61 ± 0.00^b	3.71 ± 0.01^c
Czapek Dox broth (un inoculated)	2.01 ± 0.00^e	2.01 ± 0.00^d	2.08 ± 0.00^f
Control (water)	0.65 ± 0.01^f	0.67 ± 0.00^e	0.71 ± 0.01^g

1 Data shown correspond to mean of three replications ± the standard deviation. Values in columns followed by different letters indicate significant differences according to the Tukey’s test (p < 0.05).

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DOI: https://doi.org/10.33073/pjm-2020-029 | Journal eISSN: 2544-4646 | Journal ISSN: 1733-1331

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Language: English

Page range: 379 - 383

Submitted on: Mar 23, 2020

Accepted on: Jun 18, 2020

Published on: Aug 5, 2020

Published by: Polish Society of Microbiologists

In partnership with: Paradigm Publishing Services

Publication frequency: 4 issues per year

Keywords:

Zataria multiflora,

antifungal,

endophytic fungi,

Monosporascus cannonballus

Related subjects:

Life sciences,

Microbiology and virology

© 2020 RAHIL SAID AL-BADI, THAMODINI GAYA KARUNASINGHE, ABDULLAH MOHAMMED AL-SADI, ISSA HASHIL AL-MAHMOOLI, RETHINASAMY VELAZHAHAN, published by Polish Society of Microbiologists
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Volume 69 (2020): Issue 3 (September 2020)

In vitro Antagonistic Activity of Endophytic Fungi Isolated from Shirazi Thyme (Zataria multiflora Boiss.) against Monosporascus cannonballus

Figures & Tables

Table I

Fig. 1.

Fig. 2.

Table II

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