In vitro Antagonistic Activity of Endophytic Fungi Isolated from Shirazi Thyme (Zataria multiflora Boiss.) against Monosporascus cannonballus

RAHIL SAID AL-BADI; THAMODINI GAYA KARUNASINGHE; ABDULLAH MOHAMMED AL-SADI; ISSA HASHIL AL-MAHMOOLI; RETHINASAMY VELAZHAHAN

doi:10.33073/pjm-2020-029

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In vitro Antagonistic Activity of Endophytic Fungi Isolated from Shirazi Thyme (Zataria multiflora Boiss.) against Monosporascus cannonballus

Polish Journal of Microbiology

Volume 69 (2020): Issue 3 (September 2020)

By: RAHIL SAID AL-BADI, THAMODINI GAYA KARUNASINGHE, ABDULLAH MOHAMMED AL-SADI, ISSA HASHIL AL-MAHMOOLI and RETHINASAMY VELAZHAHAN

Open Access

|Aug 2020

Abstract

Endophytic fungi viz., Nigrospora sphaerica (E1 and E6), Subramaniula cristata (E7), and Polycephalomyces sinensis (E8 and E10) were isolated from the medicinal plant, Shirazi thyme (Zataria multiflora). In in vitro tests, these endophytes inhibited the mycelial growth of Monosporascus cannonballus, a plant pathogenic fungus. Morphological abnormalities in the hyphae of M. cannonballus at the edge of the inhibition zone in dual cultures with N. sphaerica were observed. The culture filtrates of these endophytes caused leakage of electrolytes from the mycelium of M. cannonballus. To our knowledge, this is the first report on the isolation and characterization of fungal endophytes from Z. multiflora as well as their antifungal effect on M. cannonballus.

References

Al-Daghari DSS, Al-Sadi AM, Janke R, Al-Mahmooli IH, Velazhahan R. Potential of indigenous antagonistic rhizobacteria in the biological control of Monosporascus root rot and vine decline disease of muskmelon. Acta Agric Scand B-S P. 2020;70(5):371–380. https://doi.org/10.1080/09064710.2020.1748703
Search in Google Scholar Back to article
Aleandri MP, Martignoni D, Reda R, Chilosi G. Effects of preconditioning through mycorrhizal inoculation on the control of melon root rot and vine decline caused by Monosporascus cannonballus. J Phytopathol. 2015 Dec;163(11–12):898–907. https://doi.org/10.1111/jph.12389
Search in Google Scholar Back to article
Al-Rawahi AY, Al-Mahmooli IH, Al-Sadi AM, Al-Sabahi JN, Velazhahan R. Toxin production by melon root rot fungus, Monosporascus cannonballus. Australas Plant Pathol. 2018 Sep; 47(5): 543–546. https://doi.org/10.1007/s13313-018-0589-1
Search in Google Scholar Back to article
Bacon CW, White JF. Microbial Endophytes. New York (USA): Marcel Dekker Inc; 2000.
Search in Google Scholar Back to article
Batten JS, Scholthof KBG, Lovic BR, Miller ME, Martyn RD. Potential for biocontrol of Monosporascus root rot/vine decline under greenhouse conditions using hypovirulent isolates of Monosporascus cannonballus. Eur J Plant Pathol. 2000;106(7): 639–649. https://doi.org/10.1023/A:1008703410411
Search in Google Scholar Back to article
Garg H, Li H, Sivasithamparam K, Kuo J, Barbetti MJ. The infection processes of Sclerotinia sclerotiorum in cotyledon tissue of a resistant and a susceptible genotype of Brassica napus. Ann Bot (Lond). 2010 Dec;106(6):897–908. https://doi.org/10.1093/aob/mcq196
Search in Google Scholar Back to article
Goldstein J, Newbury DE, Joy DC, Lyman CE, Echlin P, Lifshin E, Sawyer L, Michael JR. Scanning Electron Microscopy and X-Ray Microanalysis, 3rd Edition. Berlin (Germany): Springer; 2003.
Search in Google Scholar Back to article
Hajlaoui MR, Benhamou N, Belanger NR. Cytochemical study of the antagonistic activity of Sporothrix flocculosa on rose powdery mildew, Sphaerotheca pannosa var. rosae. Phytopathology. 1992; 82(5):583–589. https://doi.org/10.1094/Phyto-82-583
Search in Google Scholar Back to article
Halo BA, Al-Yahyai RA, Al-Sadi AM. Aspergillus terreus inhibits growth and induces morphological abnormalities in Pythium aphanidermatum and suppresses Pythium-induced damping-off of cucumber. Front Microbiol. 2018 Feb 1;9:95. https://doi.org/10.3389/fmicb.2018.00095
Search in Google Scholar Back to article
Homthong M, Kubera A, Srihuttagum M, Hongtrakul V. Isolation and characterization of chitinase from soil fungi, Paecilomyces sp. Agric Nat Resour (Bangk). 2016 Jul;50(4):232–242. https://doi.org/10.1016/j.anres.2015.09.005
Search in Google Scholar Back to article
Jia M, Chen L, Xin HL, Zheng CJ, Rahman K, Han T, Qin LP. A friendly relationship between endophytic fungi and medicinal plants: a systematic review. Front Microbiol. 2016 Jun 09;7:906. https://doi.org/10.3389/fmicb.2016.00906
Search in Google Scholar Back to article
Kim JC, Choi GJ, Park JH, Kim HT, Cho KY. Activity against plant pathogenic fungi of phomalactone isolated from Nigrospora sphaerica. Pest Manag Sci. 2001 Jun;57(6):554–559. https://doi.org/10.1002/ps.318
Search in Google Scholar Back to article
Liu D, Coloe S, Baird R, Pederson J. Rapid mini-preparation of fungal DNA for PCR. J Clin Microbiol. 2000 Jan;38(1):471.
Search in Google Scholar Back to article
Liu YJ, Tang Q, Fang L. First report of Nigrospora sphaerica causing leaf blight on Camellia sinensis in China. Plant Dis. 2016 Jan;100(1):221. https://doi.org/10.1094/PDIS-04-15-0493-PDN
Search in Google Scholar Back to article
Lu Y, Chen C, Chen H, Zhang J, Chen W. Isolation and identification of endophytic fungi from Actinidia macrosperma and investigation of their bioactivities. Evid Based Complementary Altern Med. 2012;382742. https://doi.org/10.1155/2012/382742
Search in Google Scholar Back to article
Manhas RK, Kaur T. Biocontrol potential of Streptomyces hydrogenans strain DH16 toward Alternaria brassicicola to control damping off and black leaf spot of Raphanus sativus. Front Plant Sci. 2016 Dec 16;7:1869. https://doi.org/10.3389/fpls.2016.01869
Search in Google Scholar Back to article
Martyn RD, Miller ME. Monosporascus root rot and vine decline: an emerging disease of melons worldwide. Plant Dis. 1996;80(7):716–725. https://doi.org/10.1094/PD-80-0716
Search in Google Scholar Back to article
Mertely JC, Martyn RD, Miller ME, Bruton BD. An expanded host range for the muskmelon pathogen Monosporascus cannonballus. Plant Dis. 1993;77(7):667–673. https://doi.org/10.1094/PD-77-0667
Search in Google Scholar Back to article
Mohammadi AM, Ebrahimi A, Mahzonieh MR, Lotfalian S. Antibacterial activities of bacterial endophytes isolated from Zataria multiflora, Achillea willhelmsii and Calendula officinalis L. against some human nosocomial pathogens. Zahedan J Res Med Sci. 2016 Aug 16;18(9):e2482. https://doi.org/10.17795/zjrms-2482
Search in Google Scholar Back to article
Sajed H, Sahebkar A, Iranshahi M. Zataria multiflora Boiss. (Shirazi thyme) – an ancient condiment with modern pharmaceutical uses. J Ethnopharmacol. 2013 Feb;145(3):686–698. https://doi.org/10.1016/j.jep.2012.12.018
Search in Google Scholar Back to article
Staniek A, Woerdenbag HJ, Kayser O. Endophytes: exploiting biodiversity for the improvement of natural product-based drug discovery. J Plant Interact. 2008 Jun;3(2):75–93. https://doi.org/10.1080/17429140801886293
Search in Google Scholar Back to article
Toghueo RMK, Eke P, Zabalgogeazcoa Í, de Aldana BRV, Nana LW, Boyom FF. Biocontrol and growth enhancement potential of two endophytic Trichoderma spp. from Terminalia catappa against the causative agent of Common Bean Root Rot (Fusarium solani). Biol Control. 2016 May;96:8–20. https://doi.org/10.1016/j.biocontrol.2016.01.008
Search in Google Scholar Back to article
Wang M, Liu F, Crous PW, Cai L. Phylogenetic reassessment of Nigrospora: ubiquitous endophytes, plant and human pathogens. Persoonia – Molecular Phylogeny and Evolution of Fungi. 2017 Dec 20;39(1):118–142. https://doi.org/10.3767/persoonia.2017.39.06
Search in Google Scholar Back to article
Wang WJ, Wang XL, Li Y, Xiao SR, Kepler RM, Yao YJ. Molecular and morphological studies of Paecilomyces sinensis reveal a new clade in clavicipitaceous fungi and its new systematic position. Syst Biodivers. 2012 Jun;10(2):221–232. https://doi.org/10.1080/14772000.2012.690784
Search in Google Scholar Back to article
Wright ER, Folgado M, Rivera MC, Crelier A, Vasquez P, Lopez SE. Nigrospora sphaerica causing leaf spot and twig and shoot blight on blueberry: a new host of the pathogen. Plant Dis. 2008 Jan;92(1):171. https://doi.org/10.1094/PDIS-92-1-0171B
Search in Google Scholar Back to article
Zhang JX, Bruton BD, Howell CR, Miller ME. Potential of Trichoderma virens for biocontrol of root rot and vine decline in Cucumis melo L. caused by Monosporascus cannonballus. Subtrop Plant Sci. 1999;51:29–37.
Search in Google Scholar Back to article
Zhao JH, Zhang YL, Wang LW, Wang JY, Zhang CL. Bioactive secondary metabolites from Nigrospora sp. LLGLM003, an endophytic fungus of the medicinal plant Moringa oleifera Lam. World J Microbiol Biotechnol. 2012 May;28(5):2107–2112. https://doi.org/10.1007/s11274-012-1015-4
Search in Google Scholar Back to article

Articles in this issue

DOI: https://doi.org/10.33073/pjm-2020-029 | Journal eISSN: 2544-4646 | Journal ISSN: 1733-1331

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Language: English

Page range: 379 - 383

Submitted on: Mar 23, 2020

Accepted on: Jun 18, 2020

Published on: Aug 5, 2020

Published by: Polish Society of Microbiologists

In partnership with: Paradigm Publishing Services

Publication frequency: 4 issues per year

Keywords:

Zataria multiflora,

antifungal,

endophytic fungi,

Monosporascus cannonballus

Related subjects:

Life sciences,

Microbiology and virology

© 2020 RAHIL SAID AL-BADI, THAMODINI GAYA KARUNASINGHE, ABDULLAH MOHAMMED AL-SADI, ISSA HASHIL AL-MAHMOOLI, RETHINASAMY VELAZHAHAN, published by Polish Society of Microbiologists
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Volume 69 (2020): Issue 3 (September 2020)