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Evidence for Infections by the Same Strain of Beta 2-toxigenic Clostridium perfringens Type A Acquired in One Hospital Ward Cover

Evidence for Infections by the Same Strain of Beta 2-toxigenic Clostridium perfringens Type A Acquired in One Hospital Ward

Polish Journal of Microbiology
Volume 68 (2019): Issue 3 (September 2019)
By: DOMINIKA SALAMON,  DOROTA OCHOŃSKA,  ILONA WOJAK,  EWA MIKOŁAJCZYK,  MAŁGORZATA BULANDA and  MONIKA BRZYCHCZY-WŁOCH  
Open Access
|Sep 2019

Figures & Tables

Fig. 1.

Detection of genes encoding virulence factors of C. perfringens isolates in 2017 tested by multiplex PCR.Legend: M – size marker,1 – C. perfringens isolate no. 8567/M/17 from patient no. 22 – C. perfringens isolate no. 8554/M/17 from patient no. 13 – reference strain of C. perfringens ATCC 3624 cpb2 – gene of β2-toxin; cpa – gene of α-toxin
Detection of genes encoding virulence factors of C. perfringens isolates in 2017 tested by multiplex PCR.Legend: M – size marker,1 – C. perfringens isolate no. 8567/M/17 from patient no. 22 – C. perfringens isolate no. 8554/M/17 from patient no. 13 – reference strain of C. perfringens ATCC 3624 cpb2 – gene of β2-toxin; cpa – gene of α-toxin

Fig. 2.

Genetic profiles of C. perfringens isolates, subjected to DNA digestion by restriction enzyme SmaI with the use of pulsed-field gel electrophoresis (PFGE) and GelCompar II software.Legend: A – Comparison of two isolates from 2017; B – comparison of five isolates: three from 2015 and two from 20178554/M/17 – C. perfringens isolate isolated from patient no. 1 in 2017; ATCC 3624 – reference strain of C. perfringens8567/M/17 – C. perfringens isolate isolated from patient no. 2 in 20177143 – C. perfringens isolate isolated from patient no. 3 in 2015; 7149 – C. perfringens isolate isolated from patient no. 2 in 20157013 – C. perfringens isolate isolated from patient no. 1 in 2015
Genetic profiles of C. perfringens isolates, subjected to DNA digestion by restriction enzyme SmaI with the use of pulsed-field gel electrophoresis (PFGE) and GelCompar II software.Legend: A – Comparison of two isolates from 2017; B – comparison of five isolates: three from 2015 and two from 20178554/M/17 – C. perfringens isolate isolated from patient no. 1 in 2017; ATCC 3624 – reference strain of C. perfringens8567/M/17 – C. perfringens isolate isolated from patient no. 2 in 20177143 – C. perfringens isolate isolated from patient no. 3 in 2015; 7149 – C. perfringens isolate isolated from patient no. 2 in 20157013 – C. perfringens isolate isolated from patient no. 1 in 2015

Antibiotic susceptibility for C_ perfringens isolates (The E-test method, according to EUCAST 2017)_

Isolate no.PenicillinsCephalo sporinsCarbapenemsFluoroquinolonesGlycopeptide and lipo-glycopeptidesMacrolides, incosamidesTetracyclinesMiscellaneous agents
AMLPPRLXLCROCTXDORETPIMPMEMMXFTECVAEDATECMRD
8554/M/17 (Patient 1)MICmg/l0.0160.0160.0160.0160.0160.0160.0160.0160.0161.50.0470.500.500.5040.190.0160.032
S/RSSSSSSSSSRSSRSRSSS
8567/M/17 (Patient 2)MICmg/l0.0160.0160.0160.0160.0160.0160.0160.0160.01620.0470.500.500.5040.1250.0160.032
S/RSSSSSSSSSRSSRSRSSS
DOI: https://doi.org/10.33073/pjm-2019-035 | Journal eISSN: 2544-4646 | Journal ISSN: 1733-1331
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Language: English
Page range: 323 - 329
Submitted on: Feb 27, 2019
|
Accepted on: May 21, 2019
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Published on: Sep 3, 2019
Published by: Polish Society of Microbiologists
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
Clostridium perfringens,
beta 2 (β2)-toxin,
NSTI,
molecular diagnostics
Related subjects:
Life sciences,
Microbiology and virology

© 2019 DOMINIKA SALAMON, DOROTA OCHOŃSKA, ILONA WOJAK, EWA MIKOŁAJCZYK, MAŁGORZATA BULANDA, MONIKA BRZYCHCZY-WŁOCH, published by Polish Society of Microbiologists
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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