Table I
Antibiotic susceptibility for C. perfringens isolates (The E-test method, according to EUCAST 2017).
| Isolate no. | Penicillins | Cephalo sporins | Carbapenems | Fluoroquinolones | Glycopeptide and lipo-glycopeptides | Macrolides, incosamides | Tetracyclines | Miscellaneous agents | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AML | P | PRL | XL | CRO | CTX | DOR | ETP | IMP | MEM | MXF | TEC | VA | E | DA | TE | C | M | RD | |
| 8554/M/17 (Patient 1) | MIC mg/l | 0.016 | 0.016 | 0.016 | 0.016 | 0.016 | 0.016 | 0.016 | 0.016 | 0.016 | 1.5 | 0.047 | 0.50 | 0.50 | 0.50 | 4 | 0.19 | 0.016 | 0.032 |
| S/R | S | S | S | S | S | S | S | S | S | R | S | S | R | S | R | S | S | S | |
| 8567/M/17 (Patient 2) | MIC mg/l | 0.016 | 0.016 | 0.016 | 0.016 | 0.016 | 0.016 | 0.016 | 0.016 | 0.016 | 2 | 0.047 | 0.50 | 0.50 | 0.50 | 4 | 0.125 | 0.016 | 0.032 |
| S/R | S | S | S | S | S | S | S | S | S | R | S | S | R | S | R | S | S | S | |
1 AML - amoxicillin, P - penicillin, PRL - piperacillin, XL - amoxicillin/clavulanic acid, CRO - ceftriaxone, CTX - cefotaxime, DOR - doripenem, ETP - ertapenem, IMP - imipenem, MEM - meropenem, MXF - moxifloxacin, TEC - teicoplanin, VA - vancomycin, E - erythromycin, DA - clindamycin, TE - tetracycline, C - chloramphenicol, M - metronidazole, RD - rifampicin, MIC - Minimal Inhibitory Concentration, S/R - susceptibility, R - resistant, S - susceptible
Fig. 1.
Detection of genes encoding virulence factors of C. perfringens isolates in 2017 tested by multiplex PCR.
Legend: M – size marker,
1 – C. perfringens isolate no. 8567/M/17 from patient no. 2
2 – C. perfringens isolate no. 8554/M/17 from patient no. 1
3 – reference strain of C. perfringens ATCC 3624 cpb2 – gene of β2-toxin; cpa – gene of α-toxin
Fig. 2.
Genetic profiles of C. perfringens isolates, subjected to DNA digestion by restriction enzyme SmaI with the use of pulsed-field gel electrophoresis (PFGE) and GelCompar II software.
Legend: A – Comparison of two isolates from 2017; B – comparison of five isolates: three from 2015 and two from 2017
8554/M/17 – C. perfringens isolate isolated from patient no. 1 in 2017; ATCC 3624 – reference strain of C. perfringens
8567/M/17 – C. perfringens isolate isolated from patient no. 2 in 2017
7143 – C. perfringens isolate isolated from patient no. 3 in 2015; 7149 – C. perfringens isolate isolated from patient no. 2 in 2015
7013 – C. perfringens isolate isolated from patient no. 1 in 2015