Single Assay Detection of Acute Bee Paralysis Virus, Kashmir Bee Virus and Israeli Acute Paralysis Virus

Roy Francis; Per Kryger

doi:10.2478/v10289-012-0014-x

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Single Assay Detection of Acute Bee Paralysis Virus, Kashmir Bee Virus and Israeli Acute Paralysis Virus

Journal of Apicultural Science

Volume 56 (2012): Issue 1 (June 2012)

By: Roy Francis and Per Kryger

Open Access

|Jun 2012

Abstract

A new RT-PCR primer pair designed to identify Acute Bee Paralysis Virus (ABPV), Kashmir Bee Virus (KBV) or Israeli Acute Bee Paralysis Virus (IAPV) of honey bees (Apis mellifera L.) in a single assay is described. These primers are used to screen samples for ABPV, KBV, or IAPV in a single RT-PCR reaction saving time and money. The primers are located in the predicted overlapping gene (pog/ORFX) which is highly conserved across ABPV, KBV, IAPV and other dicistroviruses of social insects. This study has also identified the first case of IAPV in Denmark.

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Articles in this issue

DOI: https://doi.org/10.2478/v10289-012-0014-x | Journal eISSN: 2299-4831 | Journal ISSN: 1643-4439

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Language: English

Page range: 137 - 146

Published on: Jun 19, 2012

Published by: The National Institute of Horticultural Research and Apicultural Research Association

In partnership with: Paradigm Publishing Services

Keywords:

honey bee virus,

IAPV,

KBV,

ABPV,

AKI primers

Related subjects:

Life sciences,

Zoology,

Life sciences, other

© 2012 Roy Francis, Per Kryger, published by The National Institute of Horticultural Research and Apicultural Research Association
This work is licensed under the Creative Commons License.

Volume 56 (2012): Issue 1 (June 2012)