Pseudomonas as a Prospective Candidate For Minimization in use of Chemical Pesticides or their Gradual Replacement with Biocontrol Agents on Agricultural Fields

Sharma, Poornima

doi:10.2478/v10207-012-0015-6

Abstract

Isolates of Pseudomonas species obtained from rhizosphere, rhizoplane and endophytic habitats of various plants were tested for their antagonistic activity against plant pathogenic Fusarium oxysporum isolated from the roots of a diseased pea plant. Pseudomonas isolate P5 showed best activity against F. oxysporum followed by P4, P22 and P17. Some isolates of Pseudomonas, P24, P25 and P26, grew unrestrictedly and completely covered the Fusarium colony. The antagonistic effect of Pseudomonas on F. oxysporum observed in this research can be attributed to the ability of antibiotic synthesis by Pseudomonas and also the ability to counter the toxic metabolites of Fusarium oxysporum.

References

BAKER, R. 1986. Biological control: an overview. In Canadian Journal of Plant Pathology, vol. 8, 1986, no. 2, pp. 218-221.
Search in Google Scholar
BIRTHAL, P.S. - SHARMA, O.P. (Eds.) 2004. Proceedings 11 Integrated Pest Management in Indian Agriculture. Shakarpur, Delhi : Chand Press D-97, 2004, pp. 1-19.
Search in Google Scholar
BLAZEVIC, D.J. - KOEPCKE, M.H. - MATSEN, J.M. 1973. Incidence and identification of Pseudomonas fluorescens and Pseudomonas putida in the clinical laboratory. In Applied and Environmental Microbiology, vol. 25, 1973, pp. 107-110.
Search in Google Scholar
BUXTON, E.W. - STOREY, I.F. 1954. The occurrence of pea wilt in Britain. In Plant Pathology, vol. 3, 1954, no. 1, pp. 13-16.
Search in Google Scholar
BUYSENS, S. - HEUNGENS, K. - POPPE, J. - HOFTE, M. 1996. Involvement of pyochelin and pyoverdin in suppression of Pythium-induced damping-off of tomato by Pseudomonas aeruginosa 7NSK2. In Applied and Environtal Microbiology, vol. 62, 1996, no. 3, pp. 865-871.
Search in Google Scholar
CLARKSON, J.D.S. 1978. Pathogenicity of Fusarium spp. associated with foot-rots of peas and beans. In Plant Pathology, vol. 27, 1978, no. 3, pp. 110-117.
Search in Google Scholar
COMPANT, S. - DUFFY, B. - NOWAK, J. - CLEMENT, C. - BARKA, E.A. 2005. Use of plant growth-promoting bacteria for biocontrol of plant diseases: principles, mechanisms of action and future prospects. In Applied and Environmental Microbiology, vol. 71, 2005, no. 9, pp. 4951-4959. DOI: 10.1128/AEM.71.9.4951-4959.200510.1128/AEM.71.9.4951-4959.2005121460216151072
Search in Google Scholar
DHINGRA, O.D. - SINCLAIR, J.B. 1995. Basic Plant Pathology Methods (Second Ed.). Florida : CRC Press Lewis Publishes, 1995, pp. 287-303. ISBN 0-87371-638-8
Search in Google Scholar
DUFFY, B.K. - DEFAGO, G. 1997. Zinc improves biocontrol of Fusarium crown and root rot of tomato by Pseudomonas fluorescens and represses the production of pathogen metabolites inhibitory to bacterial antibiotic biosynthesis. In Phytopathology, vol. 87, 1997, no. 12, pp. 1250-1257.
Search in Google Scholar
DUIJFF, B.J. - RECORBET, G. - BAKKER, P.A.H.M. - LOPER, J.E. - LEMANCEAU, P. 1999. Microbial antagonism at the root level is involved in the suppression of Fusarium wilt by the combination of non-pathogenic Fusarium oxysporum Fo47 and Pseudomonas putida WCS358. In Phytopathology, vol. 89, 1999, no. 11, pp. 1073-1079.
Search in Google Scholar
EHLER, L.E. 2006. Perspective Integrated pest management (IPM): definition, historical development and implementation, and the other IPM. In Pest Management Science, vol. 62, 2006, no. 9, pp. 787-789. DOI: 10.1002/ps.124710.1002/ps.124716786545
Search in Google Scholar
HALLMAN, J. - QUADT-HALLMAN, A. - MAHAFEE, W.F. - KLOEPPER, J.W. 1997. Bacterial endophytes in agricultural crops. In Canadian Journal of Microbiology, vol. 43, 1997, no. 10, pp. 895-914. DOI: 10.1139/m97-13110.1139/m97-131
Search in Google Scholar
HARMAN, G.E. - HOWELL, C.R. - VITERBO, A. - CHET, I. - LORITO, M. 2004. Trichoderma species- opportunistic avirulent plant symbionts. In Natural Reviews Microbiology, vol. 2, 2004, no. 1, pp. 43-56. DOI:10.1038/nrmicro79710.1038/nrmicro79715035008
Search in Google Scholar
HASAN, H.A.H. 2002. Gibberellin and auxin production by plant root-fungi and their biosynthesis under salinitycalcium interaction. In Rostlinná výroba, vol. 48, 2002, no. 3, pp. 101-106.
Search in Google Scholar
HAWARE, M.P. 1971. Fungal microflora of seeds of Pisum sativum L. and its control. In Mycopathologia, vol. 43, 1971, no. 3-4, pp. 343-345.
Search in Google Scholar
KATAN, J. 2000. Physical and cultural methods for the management of soil-borne pathogens. In Crop Protection, vol. 19, 2000, no. 8-10, pp. 725-731.
Search in Google Scholar
KEINATH, A.P. 1994. Pathogenicity and host range of Fusarium oxysporum from sweet basil and evaluation of disease control methods. In Plant Disease, vol. 78, 1994, no. 12, pp. 1211-1215.
Search in Google Scholar
KIM, B.S. - MOON, S.S. - HWANG, B.K. 1999. Isolation, identification and antifungal activity of a macrolide antibiotic, oligomycin A, produced by Streptomyces libani. In Canadian Journal of Botany, vol. 77, 1999, pp. 850-858.
Search in Google Scholar
KING, E.O. - WARD, M.K. - RANEY, D.E. 1954. Two simple media for the demonstration of pyocyanin and fluorescein. In Journal of Laboratory and Clinical Medicine, vol. 44, 1954, pp. 401-307.
Search in Google Scholar
LANDA, B.B. - MAVRODI, O.V. - RAAIJMAKERS, J.M. - GARDENER, B.B.M. - THOMASHOW, L.S. - WELLER, D.M. 2002. Differential ability of genotypes of 2,4-diacetylphloroglucinol-producing Pseudomonas fluorescens strains to colonize the roots of pea plants. In Applied and Environmental Microbiology, vol. 68, 2002, no. 7, pp. 3226-3237. DOI:10.1128/AEM.68.7.3226-3237.200210.1128/AEM.68.7.3226-3237.200212680312088998
Search in Google Scholar
LESLIE, J.F. - SUMMERELL, B.A. 2006. The Fusarium laboratory manual. Blackwell Publishing Asia, Victoria, Australia, 2006, pp. 212-218. ISBN-10: 0-8138-1919-9
Search in Google Scholar
LOPER, J.E. - HENKELS, M.D. 1999. Utilization of heterologous siderophores enhances levels of iron available to Pseudomonas putida in the rhizosphere. In Applied and Environmental Microbiology, vol. 65, 1999, no. 12, pp. 5357-5363.
Search in Google Scholar
MANZIES, J.G. - KOCH, C. 1990. Additions to the host range of Fusarium oxysporum f.sp. radicis-lycopersici. In Plant Disease, vol. 74, 1990, no. 8, pp. 569-572.
Search in Google Scholar
MELERO-VARA, J.M. - LOPEZ-HERRERA, C.J. - BASALLOTE-UREBA, M.J. - NAVAS, J.A. - LOPEZ, M. - VELA, M.D. - GONZALEZ, L. - MORAZA, R. - PRADOS-LIGERO, A.M. 2005. Physical and chemical methods of controlling Fusarium wilt of carnation as alternatives to methyl bromide treatments. In ISHS Acta Horticulturae, vol. 698, 2005, pp. 175-180.
Search in Google Scholar
MITCHELL, R. - HURWITZ, E. 1965. Suppression of Pythium debaryanum by lytic rhizosphere bacteria. In Phytopathology, vol. 55, 1965, pp. 156-158.
Search in Google Scholar
MUKHTAR, I. 2007. Comparison of Phytochemical and chemical control of Fusarium oxysporium f. sp. ciceri. In Mycopathologia, vol. 5, 2007, no. 2, pp. 107-110.
Search in Google Scholar
NAIR, C.B. - ANITH, K.N. 2009. Efficacy of acibenzolar-S-methyl and rhizobacteria for the management of foliar blight disease of amaranth. In Journal of Tropical Agriculture, vol. 47, 2009, no. 1-2, pp. 43-47.
Search in Google Scholar
NEGI, Y.K. - GARG, S.K. - KUMAR, J. 2005. Cold-tolerant fluorescent Pseudomonas isolates from Garhwal Himalayas as potential plant growth promoting and biocontrol agents in pea. In Current Science, vol. 89, 2005, no. 12, pp. 2151-2156.
Search in Google Scholar
OKU, H. 1994. Plant pathogenesis and disease control. Florida : Lewis Publishers. CRC Press, 2004, pp. 1-39. ISBN: 0-87371-727-9
Search in Google Scholar
PADWICK, G.W. 1938. Complex fungal rotting of pea seeds. In Annals of Applied Biology, vol. 25, 1938, no. 1, pp. 100-114.
Search in Google Scholar
PANDEY, A. - PALNI, L.M.S. 1998. Isolation of Pseudomonas corrugate from Sikkim Himalaya. In World Journal of Microbioogy and Biotechnology, vol. 14, 1998, no. 3, pp. 411-413.
Search in Google Scholar
PAUL, N.C. - KIM, W.K. - WOO, S.K. - PARK, M.S. - YU, S.H. 2007. Fungal endophytes in roots of Aralia species and their antifungal activity. In Plant Pathology Journal, vol. 23, 2007, no. 4, pp. 287-294.
Search in Google Scholar
POLIZZI, G. - AIELLO, D. - GUARNACCIA, V. - VITALE, A. - PERRONE, G. - STEA, G. 2011. First report of Fusarium wilt on Philotheca myoporoides caused by Fusarium oxysporum in Italy. In Plant Disease, vol. 95, 2011, pp. 877-877.
Search in Google Scholar
SARATHCHANDRA, U. - DUGANZICH, D. - BURCH, G. 1993. Occurrence of antifungal fluorescent Pseudomonas spp. on some horticultural and pastoral plants. In New Zealand Journal of Crop and Horticultural Science, vol. 21, 1993, pp. 267-272.
Search in Google Scholar
SHALINI-SRIVASTAVA, R. 2008. Screening for antifungal activity of Pseudomonas fluorescens against phytopathogenic fungi. In The Internet Journal of Microbiology, vol. 5, 2008, no. 2. DOI: 10.5580/27dd10.5580/27dd
Search in Google Scholar
SHARMA, P. 2011. Complexity of Trichoderma-Fusarium interaction and manifestation of biological control. In Australian Journal of Crop Science, vol. 5, 2011, no. 8, pp. 1027-1038.
Search in Google Scholar
TRIGIANO, R.N. - WINDHAM, M.T. - WINDHAM, A.S. 2004. Plant Pathology - concepts and laboratory exercises. Florida : CRC Press, 2004, pp. 295-311. ISBN 0-8493-1037-7.
Search in Google Scholar
van LOON, L.C. - BAKKER, P.A.H.M. - PIETERSE, C.M.J. 1998. Systemic resistance induced by rhizosphere bacteria. In Annual Review of Phytopathology, vol. 36, 1998, pp. 453-483.
Search in Google Scholar
WALSH, J.L. - LAURENCE, M.H. - LIEW, E.C.Y. - SANGALANG, A.E. - BURGESS, L.W. - SUMMERELL, B.A. - PETROVIC, T. 2010. Fusarium: two endophytic novel species from tropical grasses of northern Australia. In Fungal Diversity, vol. 44, 2010, no. 1, pp. 149-159.
Search in Google Scholar
WHIPPS, J.M. 2001. Microbial interactions and biocontrol in the rhizosphere. In Journal of Experimental Botany, vol. 52, 2001, pp. 487-511. DOI:10.1093/jexbot/52. suppl_1.487
Search in Google Scholar
ZEILINGER, S. - OMANN, M. 2007. Trichoderma biocontrol: Signal transduction pathways involved in host sensing and mycoparasitism. In Gene Regulation System Biology, vol. 1, 2007, pp. 227-234.
Search in Google Scholar

Pseudomonas as a Prospective Candidate For Minimization in use of Chemical Pesticides or their Gradual Replacement with Biocontrol Agents on Agricultural Fields

Abstract

Paradigm

My account