Simultaneous determination of cytochrome P450 1A, 2A and 3A activities in porcine liver microsomes

Monika Johansson; Jana Tomankova; Shengjie Li; Galia Zamaratskaia

doi:10.2478/v10102-012-0024-3

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Simultaneous determination of cytochrome P450 1A, 2A and 3A activities in porcine liver microsomes

Interdisciplinary Toxicology

Volume 5 (2012): Issue 3 (September 2012)

By: Monika Johansson, Jana Tomankova, Shengjie Li and Galia Zamaratskaia

Open Access

|Jan 2013

Abstract

The aim of this study was to develop a robust method for the simultaneous determination of the activities of three porcine CYP450 enzymes in hepatic microsomes. A cocktail consisting of three selective CYP450 probe substrates, 7-ethoxyresorufin (CYP1A), coumarin (CYP2A) and 7-benzyloxy-4-trifluoromethylcoumarin (BFC; CYP3A), was incubated with porcine liver microsomes. The presence of 7-ethoxyresorufin appears to significantly influence the kinetics of coumarin hydroxylation and BFC O-debenzylation. These results indicate that the use of 7-ethoxyresorufin in substrate cocktails together with coumarin and BFC should be avoided.

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Articles in this issue

DOI: https://doi.org/10.2478/v10102-012-0024-3 | Journal eISSN: 1337-9569 | Journal ISSN: 1337-6853

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Language: English

Page range: 150 - 154

Published on: Jan 16, 2013

Published by: Slovak Academy of Sciences, Institute of Experimental Pharmacology & Toxicology, Centre of Experimental Medicine

In partnership with: Paradigm Publishing Services

Publication frequency: 4 issues per year

Keywords:

hepatic microsomes,

cytochrome P450,

multiple probe substrates,

HPLC

Related subjects:

Medicine,

Clinical medicine,

Pharmacology, toxicology

© 2013 Monika Johansson, Jana Tomankova, Shengjie Li, Galia Zamaratskaia, published by Slovak Academy of Sciences, Institute of Experimental Pharmacology & Toxicology, Centre of Experimental Medicine
This work is licensed under the Creative Commons License.

Volume 5 (2012): Issue 3 (September 2012)