Modeling of the influence of the modifier concentration on the retention process in NP-HPLC

Wojciech Zapała

doi:10.2478/v10026-008-0013-7

.blurhash-client-img { display: none !important; }

Modeling of the influence of the modifier concentration on the retention process in NP-HPLC

Green Sciences

Volume 10 (2008): Issue 1 (March 2008)

By: Wojciech Zapała

Open Access

|Apr 2008

Abstract

The adsorption model for an accurate prediction of the analyte retention in the normal - phase liquid chromatography with a binary mobile phase has been proposed. This model was derived using a thermody-namically consistent modified competitive Langmuir isotherm. The performance of the proposed equation was compared with two retention models reported in literature. All models were verified for different NP-LC systems by use of three criteria: the sum of squared differences between the experimental and theoretical data, approximation of the standard deviation and the Fisher test.

References

Schoenmakers, P. J. (1986). Optimization of Chromatographic Selectivity. A Guide to Method Development. Amsterdam, NL: Journal of Chromatography Library Vol.35, Elsevier.
Search in Google Scholar
Guiochon, G., Shirazi, S. G. & Katti, A. M. (1994). Fundamentals of preparative and nonlinear chromatography. Boston MA, USA: Academic Press.
Search in Google Scholar
Alkhamis, K. A. & Wurster, D. E. (2002). Prediction of adsorption from multicomponent solutions by activated carbon using single-solute parameters. Part II - Proposed equation.3(3), 1 - 8.
Search in Google Scholar
Prus, W. (1997). Nowe metody optymalizacji selektywności rozdziału w adsorpcyjnej i podziałowej chromatografii cieczowej. Unpublished doctoral dissertation, Silesian University, Katowice, Poland.
Search in Google Scholar
Prus, W., Vander Heyden, Y., Massart, D. L. & Kowalska, T. (1998). Modelling of solute retention in normal-phase HPLC with the chemically bonded 3-cyanopropyl stationary phase, Acta Chromatographica 8, 98 - 107.
Search in Google Scholar
Lubke, M., Le Quere, J-L. & Barron, D. (1995). Normal phase high-performance liquid chromatography of volatile compounds. Selectivity and mobile phase effects on polar bonded silica, J. Chromatogr. A 690, 41 - 54.
Open DOI Search in Google Scholar
Jandera, P., Kucerova, M. & Holikova, J. (1997). Description and prediction of retention in normal-phase high-performance liquid chromatography with binary and ternary mobile phases,762, 15 - 26.
Open DOI Search in Google Scholar
Fletcher, R. (1971). A modified Marquardt sub-routine for nonlinear least-squares. Harwell, England: AERE-R6799.
Search in Google Scholar
Kaczmarski, K., Prus, W. & Kowalska, T. (2000). Adsorption/partition model of liquid chromatography for chemically bonded stationary phases of the aliphatic cyano, reversedphase C8 and reversed-phase C18 types,869, 57 - 64.
Open DOI Search in Google Scholar
Snyder, L. R. (1968). Principles of Adsorption Chromatography. New York, USA: Marcel Dekker.
Search in Google Scholar
Soczewiński, E. (1969). Solvent composition effects in thin-layer chromatography systems of the type silica gel-electron donor solvent,41(1), 179 - 182.
Search in Google Scholar
Ajnazarova, S. L. & Kafarov, V. V. (1985). Methods for Experimental Optimization in Chemical Technology. Moscow, RUS: Vishaia Shkola.
Search in Google Scholar