Development of a radiolabeled β-human chorionic gonadotropin

Amir Jalilian; Mohammad Khoshdel; Javad Garousi; Hassan Yousefnia; Mohammad Hosseini; Saeed Rajabifar; Daryoush Sardari

doi:10.2478/v10007-009-0035-6

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Development of a radiolabeled β-human chorionic gonadotropin

Acta Pharmaceutica

Volume 59 (2009): Issue 4 (December 2009)

By: Amir Jalilian, Mohammad Khoshdel, Javad Garousi, Hassan Yousefnia, Mohammad Hosseini, Saeed Rajabifar and Daryoush Sardari

Open Access

|Nov 2009

Abstract

β-Human chorionic gonadotropin (β-hCG) was successively labeled with [⁶⁷Ga]-gallium chloride after conjugation with freshly prepared diethylenetriaminepentaacetic acid dianhydride (ccDTPA). After solid phase purification of the radiolabeled hormone, high performance liquid chromatography showed radiochemical purity higher than 95 % under optimized conditions (specific activity = 22-23 TBq mM^-1, labeling efficiency 80 %). Preliminary in vivo studies (ID g^-1, %) in male wild-type rats showed marked gonadal uptake of the tracer after 240 minutes in agreement with the biodistribution studies and reported β-hCG receptors. Target to blood ratios were 5.1 and 15.2 after 3 and 24 hours, respectively, while target to muscle ratios were 35 and 40 after 3 and 24 hours, respectively.

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Articles in this issue

DOI: https://doi.org/10.2478/v10007-009-0035-6 | Journal eISSN: 1846-9558 | Journal ISSN: 1330-0075

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Language: English, Croatian

Page range: 421 - 429

Published on: Nov 16, 2009

Published by: Croatian Pharmaceutical Society

In partnership with: Paradigm Publishing Services

Keywords:

β-hCG,

Related subjects:

© 2009 Amir Jalilian, Mohammad Khoshdel, Javad Garousi, Hassan Yousefnia, Mohammad Hosseini, Saeed Rajabifar, Daryoush Sardari, published by Croatian Pharmaceutical Society
This work is licensed under the Creative Commons License.

Volume 59 (2009): Issue 4 (December 2009)